Exon selection in α-tropomyosin mRNA is regulated by the antagonistic action of RBM4 and PTB

Jung Chun Lin, Woan Yuh Tarn

研究成果: 雜誌貢獻文章

57 引文 斯高帕斯(Scopus)

摘要

RNA-binding motif protein 4 (RBM4) has been implicated in the regulation of precursor mRNA splicing. Using differential display analysis, we identified mRNAs that associate with RBM4-containing messenger RNPs in vivo. Among these mRNAs, α-tropomyosin (α-TM) is known to exhibit a muscle cell type-specific splicing pattern. The level of the skeletal muscle-specific α-TM mRNA isoform partially correlated with that of RBM4 in human tissues examined and could be modulated by ectopic overexpression or suppression of RBM4. These results indicated that RBM4 directly influences the expression of the skeletal muscle-specific α-TM isoform. Using minigenes, we demonstrated that RBM4 can activate the selection of skeletal muscle-specific exons, possibly via binding to intronic pyrimidine-rich elements. By contrast, the splicing regulator polypyrimidine tract binding protein (PTB) excluded these exons; moreover, RBM4 antagonized this PTB-mediated exon exclusion likely by competing with PTB for binding to a CU-rich element. This study suggests a possible mechanism underlying the regulated alternative splicing of α-TM by the antagonistic splicing regulators RBM4 and PTB.

原文英語
頁(從 - 到)10111-10121
頁數11
期刊Molecular and Cellular Biology
25
發行號22
DOIs
出版狀態已發佈 - 十一月 2005
對外發佈Yes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

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