Exercise preconditioning protects against spinal cord injury in rats by upregulating neuronal and astroglial heat shock protein 72

Cheng Kuei Chang, Willy Chou, Hung Jung Lin, Yi Ching Huang, Ling Yu Tang, Mao Tsun Lin, Ching Ping Chang

研究成果: 雜誌貢獻文章

10 引文 (Scopus)

摘要

The heat shock protein 72 (HSP 72) is a universal marker of stress protein whose expression can be induced by physical exercise. Here we report that, in a localized model of spinal cord injury (SCI), exercised rats (given pre-SCI exercise) had significantly higher levels of neuronal and astroglial HSP 72, a lower functional deficit, fewer spinal cord contusions, and fewer apoptotic cells than did non-exercised rats. pSUPER plasmid expressing HSP 72 small interfering RNA (SiRNA-HSP 72) was injected into the injured spinal cords. In addition to reducing neuronal and astroglial HSP 72, the (SiRNA-HSP 72) significantly attenuated the beneficial effects of exercise preconditioning in reducing functional deficits as well as spinal cord contusion and apoptosis. Because exercise preconditioning induces increased neuronal and astroglial levels of HSP 72 in the gray matter of normal spinal cord tissue, exercise preconditioning promoted functional recovery in rats after SCI by upregulating neuronal and astroglial HSP 72 in the gray matter of the injured spinal cord. We reveal an important function of neuronal and astroglial HSP 72 in protecting neuronal and astroglial apoptosis in the injured spinal cord. We conclude that HSP 72-mediated exercise preconditioning is a promising strategy for facilitating functional recovery from SCI.

原文英語
頁(從 - 到)19018-19036
頁數19
期刊International Journal of Molecular Sciences
15
發行號10
DOIs
出版狀態已發佈 - 十月 20 2014

指紋

spinal cord injuries
HSP72 Heat-Shock Proteins
preconditioning
physical exercise
Spinal Cord Injuries
rats
Rats
shock
spinal cord
proteins
Proteins
heat
Spinal Cord
apoptosis
Cell death
Hot Temperature
Apoptosis
recovery
Recovery
plasmids

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Spectroscopy
  • Inorganic Chemistry
  • Catalysis
  • Molecular Biology
  • Computer Science Applications
  • Medicine(all)

引用此文

Exercise preconditioning protects against spinal cord injury in rats by upregulating neuronal and astroglial heat shock protein 72. / Chang, Cheng Kuei; Chou, Willy; Lin, Hung Jung; Huang, Yi Ching; Tang, Ling Yu; Lin, Mao Tsun; Chang, Ching Ping.

於: International Journal of Molecular Sciences, 卷 15, 編號 10, 20.10.2014, p. 19018-19036.

研究成果: 雜誌貢獻文章

Chang, Cheng Kuei ; Chou, Willy ; Lin, Hung Jung ; Huang, Yi Ching ; Tang, Ling Yu ; Lin, Mao Tsun ; Chang, Ching Ping. / Exercise preconditioning protects against spinal cord injury in rats by upregulating neuronal and astroglial heat shock protein 72. 於: International Journal of Molecular Sciences. 2014 ; 卷 15, 編號 10. 頁 19018-19036.
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