Ex vivo multiphoton analysis of rabbit corneal wound healing following conductive keratoplasty

Tsung Jen Wang, Wen Lo, Chiu Mei Hsueh, Ming-Shium Hsieh, Chen Yuan Dong, Fung Rong Hu

研究成果: 雜誌貢獻文章同行評審

12 引文 斯高帕斯(Scopus)

摘要

Ex vivo multiphoton imaging is used to characterize rabbit corneal wound healing after conductive keratoplasty (CK) procedures. CK is performed on the right eyes from eight New Zealand albino rabbits while the left eyes are punctured by a keratoplast tip without energy application. Rabbits are humanely sacrificed 1day, 1, 2, and 4weeks after the CK procedure. Eye balls are enucleated and placed on the microscope for multiphoton imaging. Multiphoton imaging reveals damage of corneal epithelium and stroma caused by the CK procedure and the subsequent wound healing process can be followed without histological procedures. Multiphoton excited autofluorescence images demonstrate that re-epithelilialization is accomplished within 1week in both CK and control groups. However, epithelial hyperplasia is observed in CK corneas. In addition, stromal wounds in the control group become inconspicuous within 1week while obvious wounds still exist in CK corneas for at least 4weeks. Postconductive keratoplasty corneal damage and wound healing can be characterized by multiphoton microscopy without histological procedures. Our results suggest that multiphoton microscopy has potential in the clinical evaluation of corneal damage due to refractive surgery, and can be used to study and reduce the unwanted side effects of these procedures.
原文英語
文章編號034019
期刊Journal of Biomedical Optics
13
發行號3
DOIs
出版狀態已發佈 - 2008

ASJC Scopus subject areas

  • 生物醫學工程
  • 生物材料
  • 電子、光磁材料
  • 原子與分子物理與光學
  • 放射學、核子醫學和影像學
  • 放射與超音波技術
  • 臨床生物化學

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