Ex vivo expanded circulating tumor cells for clinical anti-cancer drug prediction in patients with head and neck cancer

Kuan Chou Lin, Lai Lei Ting, Chia Lun Chang, Long Sheng Lu, Hsin Lun Lee, Fang Chi Hsu, Jeng Fong Chiou, Peng Yuan Wang, Thierry Burnouf, Dennis Chun Yu Ho, Kai Chiang Yang, Chang Yu Chen, Chu Huang Chen, Ching Zong Wu, Yin Ju Chen

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2 引文 斯高帕斯(Scopus)

摘要

The advanced-stage head and neck cancer (HNC) patients respond poorly to platinum-based treatments. Thus, a reliable pretreatment method for evaluating platinum treatment response would improve therapeutic efficiency and outcomes. This study describes a novel strategy to predict clinical drug responses in HNC patients by using eSelect, a lab-developed biomimetic cell culture system, which enables us to perform ex vivo expansion and drug sensitivity profiling of circulating tumor cells (CTCs). s. Forty liquid biopsies were collected from HNC patients, and the CTCs were expanded ex vivo using the eSelect system within four weeks. Immunofluores-cence staining confirmed that the CTC-derived organoids were positive for EpCAM and negative for CD45. Two illustrative cases present the potential of this strategy for evaluating treatment response. The statistical analysis confirmed that drug sensitivity in CTC-derived organoids was associated with a clinical response. The multivariant logistic regression model predicted that the treatment accuracy of chemotherapy responses achieved 93.75%, and the area under the curves (AUCs) of prediction models was 0.8841 in the whole dataset and 0.9167 in cisplatin specific dataset. In summary, cisplatin sensitivity profiles of patient-derived CTCs expanded ex vivo cor-relate with a clinical response to cisplatin treatment, and this can potentially underpin predictive assays to guide HNC treatments.

原文英語
文章編號6076
期刊Cancers
13
發行號23
DOIs
出版狀態已發佈 - 12月 1 2021

ASJC Scopus subject areas

  • 腫瘤科
  • 癌症研究

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