Evolution and assembly of an extremely scrambled gene

Laura F. Landweber, Tai Chih Kuo, Edward A. Curtis

研究成果: 雜誌貢獻文章同行評審

56 引文 斯高帕斯(Scopus)

摘要

The process of gene unscrambling in hypotrichous ciliates represents one of nature's ingenious solutions to the problem of gene assembly. With some essential genes scrambled in as many as 51 pieces, these ciliates rely on sequence and structural cues to rebuild their fragmented genes and genomes. Here we report the complex pattern of scrambling in the DNA polymerase α gene of Stylonychia lemnae. The germline (micronuclear) copy of this gene is broken into 48 pieces with 47 dispersed over two loci, with no asymmetry in the placement of coding segments on either strand. Direct repeats present at the boundaries between coding and noncoding sequences provide pointers to help guide assembly of the functional (macronuclear) gene. We investigate the evolution of this complex gene in three hypotrichous species.

原文英語
頁(從 - 到)3298-3303
頁數6
期刊Proceedings of the National Academy of Sciences of the United States of America
97
發行號7
DOIs
出版狀態已發佈 - 三月 28 2000
對外發佈Yes

ASJC Scopus subject areas

  • General

指紋 深入研究「Evolution and assembly of an extremely scrambled gene」主題。共同形成了獨特的指紋。

引用此