Everolimus sensitizes Ras-transformed cells to radiation in vitro through the autophagy pathway

Yu Chieh Su, Chih Chia Yu, Fei Ting Hsu, Shu Ling Fu, Jeng Jong Hwang, Ling Chien Hung, Moon Sing Lee, Wen Yen Chiou, Hon Yi Lin, Shih Kai Hung

研究成果: 雜誌貢獻文章同行評審

5 引文 斯高帕斯(Scopus)

摘要

Modern radiation therapy strives to minimize injury to organs while increasing the anticancer effects. The present study aimed to investigate the radiosensitizing effects of everolimus and to examine the molecular mechanisms responsible for everolimus-mediated radiosensitization. Radiation in combination with everolimus (30 nM) sensitized Ras-transformed cells to radiation in vitro. Radiation induced apoptotic markers (sub-G1 cell accumulation, membrane inversion and DNA fragmentation) and treatment with everolimus did not promote radiation-induced apoptosis. However, LC3-II expression increased following combination treatment with everolimus and radiation, and the radiosensitizing effects of everolimus were reversed following transfection with small interfering RNA (siRNA) targeting Beclin 1. In addition, the protein levels of activated S6 kinase 1 (S6K1) were significantly reduced following treatment with everolimus, and the phosphorylation of factor 4E binding protein 1 (4EBP1) was suppressed following combination treatment. Taken together, our data demonstrate that everolimus sensitizes Ras-transformed cells to radiation in vitro. Everolimus-mediated radiosensitization is associated with the autophagy pathway. Thus, everolimus is a novel radiosensitizing agent with potential for use in cancer radiotherapy.
原文英語
頁(從 - 到)1417-1422
頁數6
期刊International Journal of Molecular Medicine
34
發行號5
DOIs
出版狀態已發佈 - 十一月 1 2014
對外發佈

ASJC Scopus subject areas

  • 遺傳學
  • 醫藥 (全部)

指紋

深入研究「Everolimus sensitizes Ras-transformed cells to radiation in vitro through the autophagy pathway」主題。共同形成了獨特的指紋。

引用此