Evaluation of the increase in permeability of the blood-brain barrier during tumor progression after pulsed focused ultrasound

Feng Yi Yang, Hsin Ell Wang, Guan Liang Lin, Hui Hsien Lin, Tai-Tong Wong

研究成果: 雜誌貢獻文章同行評審

25 引文 斯高帕斯(Scopus)

摘要

Purpose: The purpose of this study was to evaluate the permeability of the blood-brain barrier after sonication by pulsed high-intensity focused ultrasound and to determine if such an approach increases the tumor:ipsilateral brain permeability ratio. Materials and methods: F98 glioma-bearing Fischer 344 rats were injected intravenously with Evans blue with or without blood-tumor barrier disruption induced by transcranial pulsed high-intensity focused ultrasound. Sonication was applied at a frequency of 1 MHz with a 5% duty cycle and a repetition frequency of 1 Hz. The permeability of the blood-brain barrier was assessed by the extravasation of Evans blue. Contrast-enhanced magnetic resonance images were used to monitor the gadolinium deposition path associated with transcranial pulsed high-intensity focused ultrasound, and the influencing size and location was also investigated. In addition, whole brain histological analysis was performed. The results were compared by two-tailed unpaired t-test. Results: The accumulation of Evans blue in brains and the tumor:ipsilateral brain permeability ratio of Evans blue were significantly increased after pulsed high-intensity focused ultrasound exposure. Evans blue injection followed by sonication showed an increase in the tumor:ipsilateral brain ratio of the target tumors (9.14:1) of about 2.23-fold compared with the control tumors (x4.09) on day 6 after tumor implantation. Magnetic resonance images showed that pulsed high-intensity focused ultrasound locally enhances the permeability of the blood-tumor barrier in the glioma-bearing rats. Conclusion: This method could allow enhanced synergistic effects with respect to other brain tumor treatment regimens.

原文英語
頁(從 - 到)723-730
頁數8
期刊International Journal of Nanomedicine
7
DOIs
出版狀態已發佈 - 12月 5 2012
對外發佈

ASJC Scopus subject areas

  • 生物物理學
  • 生物工程
  • 生物材料
  • 有機化學
  • 藥物發現

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