Evaluation of the increase in permeability of the blood-brain barrier during tumor progression after pulsed focused ultrasound

Feng Yi Yang, Hsin Ell Wang, Guan Liang Lin, Hui Hsien Lin, Tai-Tong Wong

研究成果: 雜誌貢獻文章

20 引文 (Scopus)

摘要

Purpose: The purpose of this study was to evaluate the permeability of the blood-brain barrier after sonication by pulsed high-intensity focused ultrasound and to determine if such an approach increases the tumor:ipsilateral brain permeability ratio. Materials and methods: F98 glioma-bearing Fischer 344 rats were injected intravenously with Evans blue with or without blood-tumor barrier disruption induced by transcranial pulsed high-intensity focused ultrasound. Sonication was applied at a frequency of 1 MHz with a 5% duty cycle and a repetition frequency of 1 Hz. The permeability of the blood-brain barrier was assessed by the extravasation of Evans blue. Contrast-enhanced magnetic resonance images were used to monitor the gadolinium deposition path associated with transcranial pulsed high-intensity focused ultrasound, and the influencing size and location was also investigated. In addition, whole brain histological analysis was performed. The results were compared by two-tailed unpaired t-test. Results: The accumulation of Evans blue in brains and the tumor:ipsilateral brain permeability ratio of Evans blue were significantly increased after pulsed high-intensity focused ultrasound exposure. Evans blue injection followed by sonication showed an increase in the tumor:ipsilateral brain ratio of the target tumors (9.14:1) of about 2.23-fold compared with the control tumors (x4.09) on day 6 after tumor implantation. Magnetic resonance images showed that pulsed high-intensity focused ultrasound locally enhances the permeability of the blood-tumor barrier in the glioma-bearing rats. Conclusion: This method could allow enhanced synergistic effects with respect to other brain tumor treatment regimens.

原文英語
頁(從 - 到)723-730
頁數8
期刊International Journal of Nanomedicine
7
DOIs
出版狀態已發佈 - 十二月 5 2012
對外發佈Yes

指紋

Evans Blue
Blood-Brain Barrier
Tumors
Permeability
Brain Neoplasms
Ultrasonics
Sonication
Brain
Neoplasms
Glioma
Bearings (structural)
Magnetic Resonance Spectroscopy
Magnetic resonance
Inbred F344 Rats
Gadolinium
Rats
Blood
Ultrasonic Waves
Injections

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Organic Chemistry
  • Drug Discovery

引用此文

Evaluation of the increase in permeability of the blood-brain barrier during tumor progression after pulsed focused ultrasound. / Yang, Feng Yi; Wang, Hsin Ell; Lin, Guan Liang; Lin, Hui Hsien; Wong, Tai-Tong.

於: International Journal of Nanomedicine, 卷 7, 05.12.2012, p. 723-730.

研究成果: 雜誌貢獻文章

@article{a741025abab540d1949340e82fdcfe03,
title = "Evaluation of the increase in permeability of the blood-brain barrier during tumor progression after pulsed focused ultrasound",
abstract = "Purpose: The purpose of this study was to evaluate the permeability of the blood-brain barrier after sonication by pulsed high-intensity focused ultrasound and to determine if such an approach increases the tumor:ipsilateral brain permeability ratio. Materials and methods: F98 glioma-bearing Fischer 344 rats were injected intravenously with Evans blue with or without blood-tumor barrier disruption induced by transcranial pulsed high-intensity focused ultrasound. Sonication was applied at a frequency of 1 MHz with a 5{\%} duty cycle and a repetition frequency of 1 Hz. The permeability of the blood-brain barrier was assessed by the extravasation of Evans blue. Contrast-enhanced magnetic resonance images were used to monitor the gadolinium deposition path associated with transcranial pulsed high-intensity focused ultrasound, and the influencing size and location was also investigated. In addition, whole brain histological analysis was performed. The results were compared by two-tailed unpaired t-test. Results: The accumulation of Evans blue in brains and the tumor:ipsilateral brain permeability ratio of Evans blue were significantly increased after pulsed high-intensity focused ultrasound exposure. Evans blue injection followed by sonication showed an increase in the tumor:ipsilateral brain ratio of the target tumors (9.14:1) of about 2.23-fold compared with the control tumors (x4.09) on day 6 after tumor implantation. Magnetic resonance images showed that pulsed high-intensity focused ultrasound locally enhances the permeability of the blood-tumor barrier in the glioma-bearing rats. Conclusion: This method could allow enhanced synergistic effects with respect to other brain tumor treatment regimens.",
keywords = "Blood-brain barrier, Brain tumor, Focused ultrasound, Glioma, Permeability",
author = "Yang, {Feng Yi} and Wang, {Hsin Ell} and Lin, {Guan Liang} and Lin, {Hui Hsien} and Tai-Tong Wong",
year = "2012",
month = "12",
day = "5",
doi = "10.2147/IJN.S28503",
language = "English",
volume = "7",
pages = "723--730",
journal = "International Journal of Nanomedicine",
issn = "1176-9114",
publisher = "Dove Medical Press Ltd.",

}

TY - JOUR

T1 - Evaluation of the increase in permeability of the blood-brain barrier during tumor progression after pulsed focused ultrasound

AU - Yang, Feng Yi

AU - Wang, Hsin Ell

AU - Lin, Guan Liang

AU - Lin, Hui Hsien

AU - Wong, Tai-Tong

PY - 2012/12/5

Y1 - 2012/12/5

N2 - Purpose: The purpose of this study was to evaluate the permeability of the blood-brain barrier after sonication by pulsed high-intensity focused ultrasound and to determine if such an approach increases the tumor:ipsilateral brain permeability ratio. Materials and methods: F98 glioma-bearing Fischer 344 rats were injected intravenously with Evans blue with or without blood-tumor barrier disruption induced by transcranial pulsed high-intensity focused ultrasound. Sonication was applied at a frequency of 1 MHz with a 5% duty cycle and a repetition frequency of 1 Hz. The permeability of the blood-brain barrier was assessed by the extravasation of Evans blue. Contrast-enhanced magnetic resonance images were used to monitor the gadolinium deposition path associated with transcranial pulsed high-intensity focused ultrasound, and the influencing size and location was also investigated. In addition, whole brain histological analysis was performed. The results were compared by two-tailed unpaired t-test. Results: The accumulation of Evans blue in brains and the tumor:ipsilateral brain permeability ratio of Evans blue were significantly increased after pulsed high-intensity focused ultrasound exposure. Evans blue injection followed by sonication showed an increase in the tumor:ipsilateral brain ratio of the target tumors (9.14:1) of about 2.23-fold compared with the control tumors (x4.09) on day 6 after tumor implantation. Magnetic resonance images showed that pulsed high-intensity focused ultrasound locally enhances the permeability of the blood-tumor barrier in the glioma-bearing rats. Conclusion: This method could allow enhanced synergistic effects with respect to other brain tumor treatment regimens.

AB - Purpose: The purpose of this study was to evaluate the permeability of the blood-brain barrier after sonication by pulsed high-intensity focused ultrasound and to determine if such an approach increases the tumor:ipsilateral brain permeability ratio. Materials and methods: F98 glioma-bearing Fischer 344 rats were injected intravenously with Evans blue with or without blood-tumor barrier disruption induced by transcranial pulsed high-intensity focused ultrasound. Sonication was applied at a frequency of 1 MHz with a 5% duty cycle and a repetition frequency of 1 Hz. The permeability of the blood-brain barrier was assessed by the extravasation of Evans blue. Contrast-enhanced magnetic resonance images were used to monitor the gadolinium deposition path associated with transcranial pulsed high-intensity focused ultrasound, and the influencing size and location was also investigated. In addition, whole brain histological analysis was performed. The results were compared by two-tailed unpaired t-test. Results: The accumulation of Evans blue in brains and the tumor:ipsilateral brain permeability ratio of Evans blue were significantly increased after pulsed high-intensity focused ultrasound exposure. Evans blue injection followed by sonication showed an increase in the tumor:ipsilateral brain ratio of the target tumors (9.14:1) of about 2.23-fold compared with the control tumors (x4.09) on day 6 after tumor implantation. Magnetic resonance images showed that pulsed high-intensity focused ultrasound locally enhances the permeability of the blood-tumor barrier in the glioma-bearing rats. Conclusion: This method could allow enhanced synergistic effects with respect to other brain tumor treatment regimens.

KW - Blood-brain barrier

KW - Brain tumor

KW - Focused ultrasound

KW - Glioma

KW - Permeability

UR - http://www.scopus.com/inward/record.url?scp=84862338450&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84862338450&partnerID=8YFLogxK

U2 - 10.2147/IJN.S28503

DO - 10.2147/IJN.S28503

M3 - Article

C2 - 22359451

AN - SCOPUS:84862338450

VL - 7

SP - 723

EP - 730

JO - International Journal of Nanomedicine

JF - International Journal of Nanomedicine

SN - 1176-9114

ER -