Evaluation of the BD GeneOhm StaphSR assay for detection of Staphylococcus aureus in patients in intensive care units

Tai Hua Ho, Yhu Chering Huang, Tzou Yien Lin

研究成果: 雜誌貢獻文章同行評審

17 引文 斯高帕斯(Scopus)

摘要

Background: Staphylococcus aureus is the major nosocomial pathogen and rapid detection of colonized patients with subsequent precaution is needed to prevent transmission. A new assay, the BD GeneOhm™ SaphSR assay (BD GeneOhm™, San Diego, CA, USA), is a multiplex real-time polymerase chain reaction (PCR) for rapid detection of both methicillin-sensitive S aureus (MSSA) as well as methicillin-resistant S aureus (MRSA). Methods: Anterior nasal swab specimens of 273 pediatric and adult patients hospitalized in intensive care units at Chang Gung Memorial Hospital were collected for this assay in parallel with conventional cultures as standard. Results: Overall, 71 (26.0%) patients were colonized with S aureus by conventional culture and MRSA accounted for 67.6% of all isolates. For the detection of MRSA, 79 patients (28.9%) were positive by PCR and 48 (17.6%) were positive by conventional cultures. The sensitivity, specificity, and positive and negative predictive values were 95.9%, 85.3%, 58.5%, 99.0%, respectively. For the detection of MSSA, 48 patients (17.6%) were positive by PCR and 23 (8.4%) were positive by conventional culture. The sensitivity, specificity, and positive and negative predictive values were 91.3%, 89.2%, 43.8%, and 99.1%, respectively. Conclusion: As a screening method, the BD GeneOhm™ StaphSR assay could rapidly detect and differentiate between MRSA and MSSA colonization. A negative result of the assay could almost exclude S aureus colonization.
原文英語
頁(從 - 到)310-315
頁數6
期刊Journal of Microbiology, Immunology and Infection
44
發行號4
DOIs
出版狀態已發佈 - 8月 2011
對外發佈

ASJC Scopus subject areas

  • 微生物學(醫學)
  • 免疫學和過敏
  • 免疫學與微生物學 (全部)
  • 傳染性疾病

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