Evaluation of recombinant fructose-1,6-bisphosphate aldolase ELISA test for the diagnosis of Schistosoma japonicum in water buffaloes

Shih Y. Peng, Kin M. Lee, John Chin Tsaihong, Po Ching Cheng, Ping Chin Fan

研究成果: 雜誌貢獻文章

15 引文 斯高帕斯(Scopus)

摘要

Fructose-1,6-bisphosphate aldolase (FBPA) is an ubiquitous enzyme essential for glycolysis, gluconeogenesis and the Calvin cycle. It has been demonstrated to induce immune responses and to be useful in the immunodiagnosis of malaria. In this study, FBPA was cloned from the adult worms of Schistosoma japonicum and tested as an antigen for the diagnosis of S. japonicum infection in water buffaloes. Enzyme-linked immunosorbent assay (ELISA) was performed on the sera from 32 infected water buffaloes and 20 negative controls using the recombinant FBPA protein or soluble worm antigen preparation (SWAP) as an antigen. The OD cut-off values were determined to be 0.57 with 100% specificity and 100% sensitivity for the FBPA ELISA and 1.13 with 93.8% specificity and 95.0% sensitivity for the SWAP ELISA. These findings indicate that the recombinant FBPA of S. japonicum should be an useful diagnostic tool for the detection of antibodies against S. japonicum.

原文英語
頁(從 - 到)527-533
頁數7
期刊Research in Veterinary Science
85
發行號3
DOIs
出版狀態已發佈 - 十二月 2008
對外發佈Yes

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

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