Evaluation of expanded bed adsorption chromatography for extraction of prothrombin complex from Cohn Supernatant I

Karl B. McCann, Peter T. Gomme, John Wu, Joseph Bertolini

研究成果: 雜誌貢獻文章

4 引文 (Scopus)

摘要

This study evaluated the feasibility of substituting expanded bed adsorption (EBA) chromatography for an existing chromatographic purification process for the isolation of prothrombin complex concentrate (PCC) from Cohn Supernatant I. The EBA chromatography (Streamline) resins were compared to the current DEAE-cellulose resin for the extraction of PCC from Cohn SNI. EBA chromatography resins efficiently bound PCC from Cohn SNI at a significantly higher flow rate of up to 300 cm/h compared to 30 cm/h for the current DEAE-cellulose process. Composition and yield of the recovered PCC reflected the elution conditions used. The results indicate that EBA chromatography could be used to efficiently produce PCC comparable to existing products.
原文英語
頁(從 - 到)227-233
頁數7
期刊Biologicals
36
發行號4
DOIs
出版狀態已發佈 - 七月 1 2008
對外發佈Yes

指紋

Prothrombin
Chromatography
Adsorption
Resins
DEAE-Cellulose
Cellulose
Purification
Feasibility Studies
Flow rate
prothrombin complex concentrates
Chemical analysis

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Infectious Diseases

引用此文

Evaluation of expanded bed adsorption chromatography for extraction of prothrombin complex from Cohn Supernatant I. / McCann, Karl B.; Gomme, Peter T.; Wu, John; Bertolini, Joseph.

於: Biologicals, 卷 36, 編號 4, 01.07.2008, p. 227-233.

研究成果: 雜誌貢獻文章

McCann, Karl B. ; Gomme, Peter T. ; Wu, John ; Bertolini, Joseph. / Evaluation of expanded bed adsorption chromatography for extraction of prothrombin complex from Cohn Supernatant I. 於: Biologicals. 2008 ; 卷 36, 編號 4. 頁 227-233.
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