Evaluation of encapsulated Newcastle disease virus liposomes using various phospholipids administered to improve chicken humoral immunity

Li Ping Tseng, Chwei Jang Chiou, Ming Chung Deng, Mei Hsiu Lin, Ryh Nan Pan, Yi You Huang, Der Zen Liu

研究成果: 雜誌貢獻文章同行評審

9 引文 斯高帕斯(Scopus)

摘要

We propose the adjuvant effects of phospholipid liposome compositions using intranasal inoculation of a liposomal-Newcastle disease virus (NDV) vaccine in chickens. The immunogenicity of three liposome formulations was determined in chickens using the hemagglutination-inhibition (HI) test, nasal secretory immunoglobulin A and serum immunoglobulin A (IgG) antibody titers using the enzyme-linked immunosorbent assay. The immune response against NDV antigens was determined after immunization with neutral charged liposomes composed of egg phosphatidylcholine (EPC) (60 μmol), cholesterol (Chol) (15 μmol), and EPC-liposomes (EPC-Lip), which elicited strong systemic (serum) and local (nasal) humoral responses. However, the intranasal administration with cationic charged liposomes composed of EPC (30 μmol), stearylamine (SA) (15 μmol), Chol (15 μmol), and SA-liposomes (SA-Lip) induced poor humoral immune responses. Only the vaccine formulated with anionic charged liposomes composed of EPC (30 μmol), dipalmitoylphosphatidylserine (15 μmol), Chol (15 μmol), and phosphatidylserine-liposomes (PS-Lip) elicited the highest titers of HI antibodies. These are the first results to suggest that antigen delivery using EPCLip is very useful in enhancing antibody production at the mucosal site and in serum.

原文英語
頁(從 - 到)621-625
頁數5
期刊Journal of Biomedical Materials Research - Part B Applied Biomaterials
91
發行號2
DOIs
出版狀態已發佈 - 十一月 2009

ASJC Scopus subject areas

  • 生物醫學工程
  • 生物材料

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