Erythromycin inhibits β2-integrins (CD11b/CD18) expression, interleukin-8 release and intracellular oxidative metabolism in neutrophils

H. C. Lin, C. H. Wang, C. Y. Liu, C. T. Yu, H. P. Kuo

研究成果: 雜誌貢獻文章同行評審

57 引文 斯高帕斯(Scopus)

摘要

Macrolides have therapeutic benefits on chronic inflammatory airway diseases. Thus, macrolides are supposed to have variable biological effects apart from antimicrobial activity. Neutrophil adherence and influx with oxidants and cytokines production implicates involvement in airway inflammation. To investigate whether erythromycin (EM) affects neutrophil activity in vitro, lipopolysaccharide (LPS)-treated neutrophils were continously incubated for 4 h in the absence or presence of increasing doses of EM from 1 μg ml-1 to 100 μg ml-1 in the last 2 h. Leukocyte adhesion molecules Mac-1 and intracellular H2O2(DCFH) were determined by flowcytometric assay. IL-8 and TNFα in supernatant was measured by ELISA method. The expression of Mac-1 and mean intracellular DCF fluorescence intensity (DCFH) of neutrophils significantly increased after stimulation with LPS. Pretreatment with EM significantly decreased LPS induced Mac-1 expression on neutrophils compared with LPS stimulation only. EM alone (100 μg ml-1) also decreased Mac-1 expression on neutrophils. EM significantly reduced the LPS-increased DCFH. EM alone (100 μg ml-1) also caused a decrease in DCFH. Increasing doses of EM also significantly decreased the IL-8 released by LPS-stimulated neutrophils. In conclusion, EM exerts a direct effect on the neutrophils by downregulating the expression of β2-integrin on neutrophils, thus leading to a decrease in the intracellular H2O2, as well as the production of IL-8. Our conclusion provides an explanation for the clinical efficacy of erythromycin in neutrophil-mediated airway inflammation.
原文英語
頁(從 - 到)654-660
頁數7
期刊Respiratory Medicine
94
發行號7
DOIs
出版狀態已發佈 - 一月 1 2000
對外發佈Yes

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

指紋 深入研究「Erythromycin inhibits β<sub>2</sub>-integrins (CD11b/CD18) expression, interleukin-8 release and intracellular oxidative metabolism in neutrophils」主題。共同形成了獨特的指紋。

引用此