Ephrin-A5 potentiates netrin-1 axon guidance by enhancing Neogenin availability

L. P. Croteau, T. J. Kao, A. Kania

研究成果: 雜誌貢獻文章

摘要

Axonal growth cones are guided by molecular cues in the extracellular environment. The mechanisms of combinatorial integration of guidance signals at the growth cone cell membrane are still being unravelled. Limb-innervating axons of vertebrate spinal lateral motor column (LMC) neurons are attracted to netrin-1 via its receptor, Neogenin, and are repelled from ephrin-A5 through its receptor EphA4. The presence of both cues elicits synergistic guidance of LMC axons, but the mechanism of this effect remains unknown. Using fluorescence immunohistochemistry, we show that ephrin-A5 increases LMC growth cone Neogenin protein levels and netrin-1 binding. This effect is enhanced by overexpressing EphA4 and is inhibited by blocking ephrin-A5-EphA4 binding. These effects have a functional consequence on LMC growth cone responses since bath addition of ephrin-A5 increases the responsiveness of LMC axons to netrin-1. Surprisingly, the overexpression of EphA4 lacking its cytoplasmic tail, also enhances Neogenin levels at the growth cone and potentiates LMC axon preference for growth on netrin-1. Since netrins and ephrins participate in a wide variety of biological processes, the enhancement of netrin-1 signalling by ephrins may have broad implications.

原文英語
文章編號12009
期刊Scientific Reports
9
發行號1
DOIs
出版狀態已發佈 - 十二月 1 2019

指紋

Ephrin-A5
Growth Cones
Axons
Ephrins
Cues
EphA4 Receptor
Biological Phenomena
Motor Neurons
Baths
Vertebrates
Tail
Extremities
Fluorescence
Immunohistochemistry
Cell Membrane
Axon Guidance
neogenin
netrin-1
Growth

ASJC Scopus subject areas

  • General

引用此文

Ephrin-A5 potentiates netrin-1 axon guidance by enhancing Neogenin availability. / Croteau, L. P.; Kao, T. J.; Kania, A.

於: Scientific Reports, 卷 9, 編號 1, 12009, 01.12.2019.

研究成果: 雜誌貢獻文章

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