Eosinophils from asthmatics release IL-5 in an autocrine fashion to prevent apoptosis through upregulation of Bcl-2 expression

Chien Da Huang, Chun Hua Wang, Chien Ying Liu, Shu Min Lin, Chun Liang Chou, Wen Te Liu, Horng Chyuan Lin, Han Pin Kuo

研究成果: 雜誌貢獻文章同行評審

17 引文 斯高帕斯(Scopus)

摘要

Interleukin (IL)-5 plays an important role in maintaining the survival of eosinophils via the specific α-subunit of its receptor. Apoptosis, a form of programmed cell death, is thought to represent a mechanism that promotes the resolution of eosinophilic inflammation in asthma. The aim of our present study is to investigate whether IL-5 acts in an autocrine fashion on eosinophil apoptosis in asthmatics. Immunoreactivities of intracellular IL-5 and IL-5 receptor α-subunit (Rα) were detected uniquely Ion the eosinophils. The magnitude of IL-5 and IL-5 Rα expression on eosinophils was significantly higher in asthmatics than that of normal subjects (p <0.05) determined by flow cytometry. Apoptosis of eosinophils was measured by the propidium iodide staining method and DNA ladder. The percent of apoptotic eosinophils from asthmatics was significantly increased by coincubation with anti-hIL-5 Rα Ab (0.1, 0.5, and 2.5 μg/mL) for 1, 2, or 16 hours than was those of corresponding controls (p <0.05, n = 8). However, there was no significant effect of anti-hIL-5 Rα Ab on eosinophil apoptosis in normal subjects. Furthermore, the expression of B-cell lymphoma-2 (Bcl-2) proteins was significantly inhibited by the anti-hIL-5 Rα Ab or antisense IL-5 oligonucleotides in asthmatics (p <0.05, n = 8), but there was no significant change in eosinophils from normal subjects. This study demonstrates that eosinophils from asthmatics release IL-5 in an autocrine fashion to act on their own IL-5 receptors in prevention of apoptosis through the upregulation of Bcl-2 expression.
原文英語
頁(從 - 到)395-403
頁數9
期刊Journal of Asthma
42
發行號5
DOIs
出版狀態已發佈 - 2005
對外發佈Yes

Keywords

  • Apoptosis
  • Asthma
  • Autocrine
  • Bcl-2
  • Eosinophil
  • Interleukin-5

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

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