Enumerating circulating tumor cells with a Self-Assembled Cell Array (SACA) Chip

A feasibility study in patients with colorectal cancer

Hsueh Yao Chu, Long Sheng Lu, Wanying Cho, Shin Yao Wu, Yu Cheng Chang, Chien Ping Lin, Chih Yung Yang, Chi Hung Lin, Jeng Kai Jiang, Fan Gang Tseng

研究成果: 雜誌貢獻文章

摘要

Colorectal cancer (CRC) is the secondmost common cause of cancer-related deathworldwide. Detecting and enumerating circulating tumor cells (CTCs) in patients with colorectal cancer emerged as an important prognostic tool which provides a direct estimate of metastatic potential. Improving the turnaround time and decreasing sample volume is critical for incorporating this liquid biopsy tool into routine practice. The objective of the current study was to validate the clinical feasibility of a self-assembled cell array (SACA) chip, a CTC counting platform with less than 4 h turnaround time, in patients with newly diagnosed colorectal cancers. In total, 179 patients with newly diagnosed colorectal cancers from a single institute were enrolled. Epithelial cell adhesion molecule positive (EpCAM(+)), cluster of differentiation 45 negative (CD45(-)) cells were isolated and enumerated from 2 mL of peripheral vein blood (PB) and inferior mesenteric vein blood (IMV) samples obtained during surgery. We found that the CTC count in PB but not IMV correlates with disease stages. Neoadjuvant chemotherapy did not lead to decreased CTC count in both types of blood samples. With cutoffs of four CTCs per 2 mL of blood, and serum carcinoembryonic antigen (CEA) level of 5 ng/mL, patients with non-metastatic disease were more likely to experience recurrence if they had high PB CTC count and high serum CEA concentration (odds ratio, 8.9). Our study demonstrates the feasibility of enumerating CTCs with a SACA chip in patients with colorectal cancer.
原文英語
文章編號56
期刊Cancers
11
發行號1
DOIs
出版狀態已發佈 - 一月 1 2019

指紋

Circulating Neoplastic Cells
Feasibility Studies
Colorectal Neoplasms
Mesenteric Veins
Veins
Cell Count
Carcinoembryonic Antigen
Serum
Blood Cells
Odds Ratio
Biopsy
Recurrence
Drug Therapy

Keywords

  • Cancer metastasis
  • Circulating tumor cell (CTC)
  • Colorectal cancer (CRC)
  • Liquid biopsy

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

引用此文

Enumerating circulating tumor cells with a Self-Assembled Cell Array (SACA) Chip : A feasibility study in patients with colorectal cancer. / Chu, Hsueh Yao; Lu, Long Sheng; Cho, Wanying; Wu, Shin Yao; Chang, Yu Cheng; Lin, Chien Ping; Yang, Chih Yung; Lin, Chi Hung; Jiang, Jeng Kai; Tseng, Fan Gang.

於: Cancers, 卷 11, 編號 1, 56, 01.01.2019.

研究成果: 雜誌貢獻文章

Chu, Hsueh Yao ; Lu, Long Sheng ; Cho, Wanying ; Wu, Shin Yao ; Chang, Yu Cheng ; Lin, Chien Ping ; Yang, Chih Yung ; Lin, Chi Hung ; Jiang, Jeng Kai ; Tseng, Fan Gang. / Enumerating circulating tumor cells with a Self-Assembled Cell Array (SACA) Chip : A feasibility study in patients with colorectal cancer. 於: Cancers. 2019 ; 卷 11, 編號 1.
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abstract = "Colorectal cancer (CRC) is the secondmost common cause of cancer-related deathworldwide. Detecting and enumerating circulating tumor cells (CTCs) in patients with colorectal cancer emerged as an important prognostic tool which provides a direct estimate of metastatic potential. Improving the turnaround time and decreasing sample volume is critical for incorporating this liquid biopsy tool into routine practice. The objective of the current study was to validate the clinical feasibility of a self-assembled cell array (SACA) chip, a CTC counting platform with less than 4 h turnaround time, in patients with newly diagnosed colorectal cancers. In total, 179 patients with newly diagnosed colorectal cancers from a single institute were enrolled. Epithelial cell adhesion molecule positive (EpCAM(+)), cluster of differentiation 45 negative (CD45(-)) cells were isolated and enumerated from 2 mL of peripheral vein blood (PB) and inferior mesenteric vein blood (IMV) samples obtained during surgery. We found that the CTC count in PB but not IMV correlates with disease stages. Neoadjuvant chemotherapy did not lead to decreased CTC count in both types of blood samples. With cutoffs of four CTCs per 2 mL of blood, and serum carcinoembryonic antigen (CEA) level of 5 ng/mL, patients with non-metastatic disease were more likely to experience recurrence if they had high PB CTC count and high serum CEA concentration (odds ratio, 8.9). Our study demonstrates the feasibility of enumerating CTCs with a SACA chip in patients with colorectal cancer.",
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AU - Cho, Wanying

AU - Wu, Shin Yao

AU - Chang, Yu Cheng

AU - Lin, Chien Ping

AU - Yang, Chih Yung

AU - Lin, Chi Hung

AU - Jiang, Jeng Kai

AU - Tseng, Fan Gang

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AB - Colorectal cancer (CRC) is the secondmost common cause of cancer-related deathworldwide. Detecting and enumerating circulating tumor cells (CTCs) in patients with colorectal cancer emerged as an important prognostic tool which provides a direct estimate of metastatic potential. Improving the turnaround time and decreasing sample volume is critical for incorporating this liquid biopsy tool into routine practice. The objective of the current study was to validate the clinical feasibility of a self-assembled cell array (SACA) chip, a CTC counting platform with less than 4 h turnaround time, in patients with newly diagnosed colorectal cancers. In total, 179 patients with newly diagnosed colorectal cancers from a single institute were enrolled. Epithelial cell adhesion molecule positive (EpCAM(+)), cluster of differentiation 45 negative (CD45(-)) cells were isolated and enumerated from 2 mL of peripheral vein blood (PB) and inferior mesenteric vein blood (IMV) samples obtained during surgery. We found that the CTC count in PB but not IMV correlates with disease stages. Neoadjuvant chemotherapy did not lead to decreased CTC count in both types of blood samples. With cutoffs of four CTCs per 2 mL of blood, and serum carcinoembryonic antigen (CEA) level of 5 ng/mL, patients with non-metastatic disease were more likely to experience recurrence if they had high PB CTC count and high serum CEA concentration (odds ratio, 8.9). Our study demonstrates the feasibility of enumerating CTCs with a SACA chip in patients with colorectal cancer.

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