Enrichment and depletion of hela topoisomerase I recognition sites among specific types of DNA elements

Carlos Perez-Stable, Chein Celia Shen, Che Kun Shen

研究成果: 雜誌貢獻文章同行評審

27 引文 斯高帕斯(Scopus)

摘要

The SDS-induced nicking of DNA helix by Hela topoisomerase I in vitro haa been studied by using 2.9 kb of cloned human DNA as the substrate. The frequency of nicking is increased from 1/23 (nick/nt) to 1/19 (nick/nt) when camptothecin is present in the nicking reaction. The cytotoxic drug also induces DNA nicks without the addition of SDS. Although the consensus built from DNA sequences from -20 to +20 of more tham one hundred of the nicking sites only shows a preference for T at position -1, the distributions of the topoisomerae I-cleavable sites among different categories of specific DNA sequences are apparently non- random. Long stretches of tandem (CA), A, or T residues, and the GC-rich promoter region of C globin gene are all refractory to the nicking reaction. Howevar, the nicking frequencies of short direct repeats flanking different Alu type sequences are as high as 1/6 (nick/nt). Finally, several tandemly arranged minirepeats of the form (TA), that are usually found at the 3' ends of the primate Alu family xKpynll family repeats, can be cleaved efficiently in a regular pattern by the enzyme. These data are discussed in terms of the mode of recognition of DNA seqeunces/structures by topoisomerase I, and its possible roles in the nonhomologous insertion of repetitive DNA sequences.

原文英語
頁(從 - 到)7975-7993
頁數19
期刊Nucleic Acids Research
16
發行號16
DOIs
出版狀態已發佈 - 八月 25 1988
對外發佈Yes

ASJC Scopus subject areas

  • Genetics

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