Enhancement of Transactivation Activity of Rta of Epstein-Barr Virus by RanBPM

Li Kwan Chang, Shih Tung Liu, Chung Wen Kuo, Wen Hung Wang, Jian Ying Chuang, Elisabetta Bianchi, Yi Ren Hong

研究成果: 雜誌貢獻文章同行評審

34 引文 斯高帕斯(Scopus)

摘要

Epstein-Barr virus (EBV) expresses the immediate-early protein Rta to activate the transcription of EBV lytic genes and the lytic cycle. We show that RanBPM acts as a binding partner of Rta in yeast two-hybrid analysis. The binding was confirmed by glutathione-S-transferase pull-down assay. A coimmunoprecipitation experiment and confocal microscopy revealed that RanBPM and Rta interact in vivo and colocalize in the nucleus. The interaction appears to involve the SPRY domain in RanBPM and the region between amino acid residues 416 to 476 in Rta. The interaction promotes the transactivation activity of Rta in activating the transcription of BMLF1 and p21 in transient transfection assays. Additionally, RanBPM interacts with SUMO-E2 (Ubc9) to promote sumoylation of Rta by SUMO-1. This fact explains why the expression of RanBPM enhances the transactivation activity of Rta. Taken together, the present results indicate a new role of RanBPM in regulating a viral protein that is critical to EBV lytic activation.
原文英語
頁(從 - 到)231-242
頁數12
期刊Journal of Molecular Biology
379
發行號2
DOIs
出版狀態已發佈 - 五月 29 2008
對外發佈

ASJC Scopus subject areas

  • 生物物理學
  • 結構生物學
  • 分子生物學

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