Enhancement of primary and secondary cellular immune responses against human immunodeficiency virus type 1 Gag by using DNA expression vectors that target Gag antigen to the secretory pathway

Jian Tai Qiu, Bindong Liu, Chunjuan Tian, George N. Pavlakis, Xiao Fang Yu

研究成果: 雜誌貢獻文章同行評審

64 引文 斯高帕斯(Scopus)

摘要

In this study, we have investigated the influence of antigen targeting after DNA vaccination upon the induction of cellular immune responses against human immunodeficiency virus type 1 (HIV-1) Gag. In addition to the standard version of HIV-1 Gag, we constructed Gag expression vectors that encode a secreted (Sc-Gag) and a cytoplasmic (Cy-Gag) Gag molecule. Although all three HIV-1 Gag expression vectors induced detectable humoral and cellular immune responses, after intramuscular injection the DNA vector encoding the Sc-Gag generated the highest primary cytotoxic T-lymphocyte (CTL) and T-helper responses. Mice immunized with one of the HIV-1 Gag DNA vectors (but not with the control vector pcDNA3.1) developed a protective immune response against infection with recombinant vaccinia virus expressing HIV-1 Gag, and this response persisted for 125 days. The magnitude of the protection correlated with the levels of Gag-specific ex vivo CTL activity and the number of CD8+ T cells producing gamma interferon. The DNA vector encoding the Sc-Gag induced higher levels of protection and greater secondary CTL responses than did the DNA vector encoding Cy-Gag.

原文英語
頁(從 - 到)5997-6005
頁數9
期刊Journal of Virology
74
發行號13
DOIs
出版狀態已發佈 - 2000
對外發佈

ASJC Scopus subject areas

  • 微生物學
  • 免疫學
  • 昆蟲科學
  • 病毒學

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