Enhancement of endogenous production of 12-L-hydroxy-5, 8, 10, 14-eicosatetraenoic acid in aortic smooth muscle cells by platelet-derived growth factor

Junko Nakao, Yasuko Koshihara, Hideki Ito, Sei itsu Murota, Wen Chang Chang

研究成果: 雜誌貢獻文章

13 引文 斯高帕斯(Scopus)

摘要

Platelet-derived growth factor (PDGF) has a chemotactic effect on smooth muscle cells, which is inhibited by lipoxygenase inhibitor caffeic acid. In order to study the role of endogenous lipoxygenase products of arachidonic acid on the chemotactic action of PDGF, effects of PDGF on the lipoxygenase pathway in smooth muscle cells were examined. Lipoxygenase products were analyzed by high-performance liquid chromatography. 15-, 5- and 12-lipoxygenase activities, in order of magnitude, were found in smooth muscle cell homogenate. However, when the lipoxygenase products were analyzed using intact cells prelabelled with [14C] arachidonic acid, only 12-L-hydroxy-5, 8, 10, 14-eicosatetraenoic acid (HETE) was found to be produced endogenously. In addition, 12-HETE was not released into the medium. Treatment of the cells with PDGF increased the endogenous production of 12-HETE. The amounts of intracellular 12-HETE in PDGF-treated cells were 126, 132 and 146% at 1,3, and 10 hr's after the initiation of PDGF treatment, respectively, when control value at each time point was considered as 100%. Caffeic acid (10-4M) completely inhibited the PDGF effect on 12-HETE production. However, PDGF treatment did not significantly alter the 12-lipoxygenase activity. These results suggest that the stimulatory effect of PDGF on 12-HETE production was not mediated by the activation of 12-lipoxygenase activity. Since 12-HETE itself is a potent chemoattractant for smooth muscle cells, the present data strongly suggest that 12-HETE could be an important intracellular mediator of the chemotactic action of PDGF on aortic smooth muscle cells.

原文英語
頁(從 - 到)1435-1442
頁數8
期刊Life Sciences
37
發行號15
DOIs
出版狀態已發佈 - 十月 14 1985
對外發佈Yes

ASJC Scopus subject areas

  • Pharmacology

指紋 深入研究「Enhancement of endogenous production of 12-L-hydroxy-5, 8, 10, 14-eicosatetraenoic acid in aortic smooth muscle cells by platelet-derived growth factor」主題。共同形成了獨特的指紋。

  • 引用此