Enhancement of biocompatibility on bioactive titanium surface by low-temperature plasma treatment

Chia Cheng Lin, Hsin Chung Cheng, Chiung Fang Huang, Che Tong Lin, Sheng Yang Lee, Chin Sung Chen, Keng Liang Ou

研究成果: 雜誌貢獻文章

25 引文 (Scopus)

摘要

The surface of implantable biomaterials directly contacts the host tissue and is critical in determining biocompatibility. To improve implant integration, interfacial reactions must be controlled to minimize nonspecific adsorption of proteins, and tissue-healing phenomena can be controlled. The purpose of this study was to develop a new method of functionalizing titanium surfaces by plasma treatment. The covalent immobilization of bioactive organic molecules and the bioactivities in vitro were assessed by transmission electron microscopy (TEM), atomic force spectroscopy (AFM), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), and 3-(4,5-dimethylthiazole-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay as indices of cellular cytotoxicity. Argon plasma removed all of the adsorbed contaminants and impurities. Plasma-cleaned titanium surfaces showed better bioactive performances than untreated titanium surfaces. The analytical results reveal that plasma-cleaned titanium surfaces provide a clean and reproducible starting condition for further plasma treatments to create well-controlled surface layers. Allylamine was ionized by plasma treatment, and acted as a medium to link albumin. Cells demonstrated a good spread, and a wide attachment was attained on the Albu-Ti plate. Cell attachment and growth were shown to be influenced by the surface properties. The plasma treatment process plays an important role in facilitating tissue healing. This process not only provides a clean titanium surface, but also leads to surface amination on plasma-treated titanium surfaces. Surface cleaning by ion bombardment and surface modification by plasma polymerization are believed to remove contamination on titanium surfaces and thus promote tissue healing.
原文英語
頁(從 - 到)8590-8598
頁數9
期刊Japanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers
44
發行號12
DOIs
出版狀態已發佈 - 十二月 8 2005
對外發佈Yes

指紋

biocompatibility
cold plasmas
activity (biology)
titanium
augmentation
healing
attachment
argon plasma
cells
immobilization
albumins
surface properties
cleaning
contaminants
bombardment
bromides
surface layers
contamination
polymerization
photoelectron spectroscopy

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)

引用此文

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title = "Enhancement of biocompatibility on bioactive titanium surface by low-temperature plasma treatment",
abstract = "The surface of implantable biomaterials directly contacts the host tissue and is critical in determining biocompatibility. To improve implant integration, interfacial reactions must be controlled to minimize nonspecific adsorption of proteins, and tissue-healing phenomena can be controlled. The purpose of this study was to develop a new method of functionalizing titanium surfaces by plasma treatment. The covalent immobilization of bioactive organic molecules and the bioactivities in vitro were assessed by transmission electron microscopy (TEM), atomic force spectroscopy (AFM), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), and 3-(4,5-dimethylthiazole-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay as indices of cellular cytotoxicity. Argon plasma removed all of the adsorbed contaminants and impurities. Plasma-cleaned titanium surfaces showed better bioactive performances than untreated titanium surfaces. The analytical results reveal that plasma-cleaned titanium surfaces provide a clean and reproducible starting condition for further plasma treatments to create well-controlled surface layers. Allylamine was ionized by plasma treatment, and acted as a medium to link albumin. Cells demonstrated a good spread, and a wide attachment was attained on the Albu-Ti plate. Cell attachment and growth were shown to be influenced by the surface properties. The plasma treatment process plays an important role in facilitating tissue healing. This process not only provides a clean titanium surface, but also leads to surface amination on plasma-treated titanium surfaces. Surface cleaning by ion bombardment and surface modification by plasma polymerization are believed to remove contamination on titanium surfaces and thus promote tissue healing.",
keywords = "Albumin, Allyamine, Biocompatibility, Plasma polymerization, Tissue healing, Titanium, Adsorption, Atomic force microscopy, Biomaterials, Bromine compounds, Cell immobilization, Cytology, Impurities, Plasma applications, Proteins, Scanning electron microscopy, Tissue, Toxicity, Transmission electron microscopy, X ray photoelectron spectroscopy, Plasma treatment",
author = "Lin, {Chia Cheng} and Cheng, {Hsin Chung} and Huang, {Chiung Fang} and Lin, {Che Tong} and Lee, {Sheng Yang} and Chen, {Chin Sung} and Ou, {Keng Liang}",
note = "被引用次數:22 Export Date: 10 August 2016 CODEN: JAPND 通訊地址: Ou, K.-L.; Graduate Institute of Oral Sciences, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan; 電子郵件: klou@tmu.edu.tw 參考文獻: Ratner, B.D., (1995) Surf. Interface Anal., 23, p. 521; Lausmaa, J., Kasemo, B., Rolander, U., Bjursten, L.M., Ericson, L.E., Rosander, L., Thomsen, P., (1988) Surface Characterization of Biomaterials, p. 161. , ed. B. D. Ratner (Elsevier Science, Amsterdam); Ratner, B.D., (1993) J. Biomed. Mater. Res., 27, p. 837; Ou, K.L., Wu, W.F., Chou, C.P., Chiou, C.S., Wu, C.C., (2002) J. Vac. Sci. Technol. B, 20, p. 2154; Chang, K.M., Yeh, T.H., Deng, I.C., Shih, C.W., (1997) J. Appl. Phys., 82, p. 1469; Yang, W.L., Wu, W.F., Liu, D.G., Wu, C.C., Ou, K.L., (2001) Solid-state Electron., 45, p. 149; Ou, K.L., Wu, C.C., Hsu, C.C., Chen, C.S., Shyng, Y.C., Wu, W.F., (2005) Microelectron. Eng., 81, p. 44; Ou, K.L., Chiou, S.Y., Lin, M.H., Hsu, R.Q., (2005) J. Electrochem. Soc., 152, pp. G138; Gombotz, W.R., Hoffman, A.S., (1987) CRC Crit. Rev. Biocompat., 4, p. 1; Kasemo, B., Lausmaa, J., (1988) J. Biomed. Mater. Res., 22, p. 145; Keller, J.C., Draughn, R.A., Wightman, J.P., Dougherty, W.J., Meletiou, S.D., (1990) Int. J. Oral Maxillofac. Implants, 5, p. 360; Smith, D.C., Pilliar, R.M., Metson, J.B., McIntyre, N.S., (1991) J. Biomed. Mater. Res., 25, p. 1069; Swart, K.M., Keller, J.C., Wightman, J.P., Draughn, R.A., Stanford, C.M., Michaels, C.M., (1992) J. Oral Implantol., 18, p. 130; Baier, R.E., Meyer, A.E., Akers, C.K., Natiella, J.R., Meenaghan, M., Carter, J.M., (1982) Biomaterials, 3, p. 241; Baier, R.E., Meyer, A.E., (1983) Physicochemical Aspects of Polymer Surfaces, 2, p. 895. , ed. K. L. Mittal (Plenum Press, New York); Carllon, L.V., Albrektsson, T., Bergman, C., (1989) Int. J. Oral Maxillofac. Implants, 4, p. 199; Doundoulakis, D.M.D., (1987) J. Prosthet. Dent., 58, p. 471; Kummer, F.J., Ricci, J.L., Blumenthal, N.C., (1992) J. Appl. Biomater., 3, p. 39; Rowland, S.A., Shalaby, S.W., Latour, R.A.J., Recum, A.Fv., (1995) J. Appl. Biomater., 7, p. 1; Stanford, C.M., Keller, J.C., Solrush, M., (1994) J. Dent. Res., 73, p. 1061; Aronsson, B.O., Lausmaa, J., Kasemo, B., (1997) J. Biomed. Mater. Res., 35, p. 49; Larsson, C., Thomsen, P., Lausmaa, J., Rodahl, M., Kasemo, B., Ericson, L.E., (1994) Biomaterials, 15, p. 1062; Thomas, K.A., Kay, J.F., Cook, S.D., Jarcho, M., (1987) J. Biomed. Mater. Res., 21, p. 1395; Ricci, J.L., Spivak, J.M., Blumenthal, N.C., Alexander, H., (1991) The Bone-biomaterial Interface, p. 334. , ed. J. E. Davies (University of Toronto Press, Toronto); Groessner-Schreiber, B., Tuan, R.S., (1992) J. Cell Sci., 101, p. 209; Serro, A.P., Fernandes, A.C., Saramago, B., Lima, J., Barbosa, M.A., (1997) Biomaterials, 18, p. 963; Moulder, J.F., Stickle, W.F., Sobol, P.E., Bomben, K.D., (1995) Handbook of X-ray Photoelectron Spectroscopy, p. 170. , Physical Electronics, Eden Prairie; Lausmaa, J., Kasemo, B., Mattsson, H., Odelius, H., (1990) Appl. Surf. Sci., 45, p. 189; Diks, A., (1981) Electron Spectroscopy: Theory, Techniques and Applications, 4. , eds, C. R. Brundle and A. D. Baker (Academic Press, London), Chap. 5; Armstrong, N.R., Quinn, A.D., (1977) Surf. Sci., 67, p. 451; G{\"o}pel, W., Anderson, J.A., Frankel, D., Jaenig, M., Phillips, K., Ach{\"a}afer, J.A., Rocker, G., (1984) Surf. Sci., 139, p. 333; Ancarz, I.G., Pozniak, G., Bryjak, M., Tylus, W., (2002) Eur. Polym. J., 38, p. 1937; Ratner, B.D., (1993) J. Biomed. Mater. Res., 27, p. 837; Park, J.Y., Gemmell, C.H., Davies, J.E., (2001) Biomaterials, 22, p. 2671; Maitz, M.F., Pham, M.T., Wieser, E., Tsyganov, I., (2003) J. Biomater. Appl., 17, p. 303; Sunny, M.C., Sharma, C.P., (1991) J. Biomater, Appl., 6, p. 89; Mustafa, K., Wroblewski, J., Hultenby, K., Silva Lopez, B., Arvidson, K., (2000) Clin. Oral Implant Res., 2, p. 116; Puleo, D.A., Kissling, R.A., Sheu, M.S., (2002) Biomaterials, 23, p. 2079; Qombotz, W.R., Huffman, A.S., (1988) J. Appl. Polym. Sci. Appl. Polym. Symp., 42, p. 285",
year = "2005",
month = "12",
day = "8",
doi = "10.1143/JJAP.44.8590",
language = "English",
volume = "44",
pages = "8590--8598",
journal = "Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes",
issn = "0021-4922",
publisher = "Japan Society of Applied Physics",
number = "12",

}

TY - JOUR

T1 - Enhancement of biocompatibility on bioactive titanium surface by low-temperature plasma treatment

AU - Lin, Chia Cheng

AU - Cheng, Hsin Chung

AU - Huang, Chiung Fang

AU - Lin, Che Tong

AU - Lee, Sheng Yang

AU - Chen, Chin Sung

AU - Ou, Keng Liang

N1 - 被引用次數:22 Export Date: 10 August 2016 CODEN: JAPND 通訊地址: Ou, K.-L.; Graduate Institute of Oral Sciences, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan; 電子郵件: klou@tmu.edu.tw 參考文獻: Ratner, B.D., (1995) Surf. Interface Anal., 23, p. 521; Lausmaa, J., Kasemo, B., Rolander, U., Bjursten, L.M., Ericson, L.E., Rosander, L., Thomsen, P., (1988) Surface Characterization of Biomaterials, p. 161. , ed. B. D. Ratner (Elsevier Science, Amsterdam); Ratner, B.D., (1993) J. Biomed. Mater. Res., 27, p. 837; Ou, K.L., Wu, W.F., Chou, C.P., Chiou, C.S., Wu, C.C., (2002) J. Vac. Sci. Technol. B, 20, p. 2154; Chang, K.M., Yeh, T.H., Deng, I.C., Shih, C.W., (1997) J. Appl. Phys., 82, p. 1469; Yang, W.L., Wu, W.F., Liu, D.G., Wu, C.C., Ou, K.L., (2001) Solid-state Electron., 45, p. 149; Ou, K.L., Wu, C.C., Hsu, C.C., Chen, C.S., Shyng, Y.C., Wu, W.F., (2005) Microelectron. Eng., 81, p. 44; Ou, K.L., Chiou, S.Y., Lin, M.H., Hsu, R.Q., (2005) J. Electrochem. Soc., 152, pp. G138; Gombotz, W.R., Hoffman, A.S., (1987) CRC Crit. Rev. Biocompat., 4, p. 1; Kasemo, B., Lausmaa, J., (1988) J. Biomed. Mater. Res., 22, p. 145; Keller, J.C., Draughn, R.A., Wightman, J.P., Dougherty, W.J., Meletiou, S.D., (1990) Int. J. Oral Maxillofac. Implants, 5, p. 360; Smith, D.C., Pilliar, R.M., Metson, J.B., McIntyre, N.S., (1991) J. Biomed. Mater. Res., 25, p. 1069; Swart, K.M., Keller, J.C., Wightman, J.P., Draughn, R.A., Stanford, C.M., Michaels, C.M., (1992) J. Oral Implantol., 18, p. 130; Baier, R.E., Meyer, A.E., Akers, C.K., Natiella, J.R., Meenaghan, M., Carter, J.M., (1982) Biomaterials, 3, p. 241; Baier, R.E., Meyer, A.E., (1983) Physicochemical Aspects of Polymer Surfaces, 2, p. 895. , ed. K. L. Mittal (Plenum Press, New York); Carllon, L.V., Albrektsson, T., Bergman, C., (1989) Int. J. Oral Maxillofac. Implants, 4, p. 199; Doundoulakis, D.M.D., (1987) J. Prosthet. Dent., 58, p. 471; Kummer, F.J., Ricci, J.L., Blumenthal, N.C., (1992) J. Appl. Biomater., 3, p. 39; Rowland, S.A., Shalaby, S.W., Latour, R.A.J., Recum, A.Fv., (1995) J. Appl. Biomater., 7, p. 1; Stanford, C.M., Keller, J.C., Solrush, M., (1994) J. Dent. Res., 73, p. 1061; Aronsson, B.O., Lausmaa, J., Kasemo, B., (1997) J. Biomed. Mater. Res., 35, p. 49; Larsson, C., Thomsen, P., Lausmaa, J., Rodahl, M., Kasemo, B., Ericson, L.E., (1994) Biomaterials, 15, p. 1062; Thomas, K.A., Kay, J.F., Cook, S.D., Jarcho, M., (1987) J. Biomed. Mater. Res., 21, p. 1395; Ricci, J.L., Spivak, J.M., Blumenthal, N.C., Alexander, H., (1991) The Bone-biomaterial Interface, p. 334. , ed. J. E. Davies (University of Toronto Press, Toronto); Groessner-Schreiber, B., Tuan, R.S., (1992) J. Cell Sci., 101, p. 209; Serro, A.P., Fernandes, A.C., Saramago, B., Lima, J., Barbosa, M.A., (1997) Biomaterials, 18, p. 963; Moulder, J.F., Stickle, W.F., Sobol, P.E., Bomben, K.D., (1995) Handbook of X-ray Photoelectron Spectroscopy, p. 170. , Physical Electronics, Eden Prairie; Lausmaa, J., Kasemo, B., Mattsson, H., Odelius, H., (1990) Appl. Surf. Sci., 45, p. 189; Diks, A., (1981) Electron Spectroscopy: Theory, Techniques and Applications, 4. , eds, C. R. Brundle and A. D. Baker (Academic Press, London), Chap. 5; Armstrong, N.R., Quinn, A.D., (1977) Surf. Sci., 67, p. 451; Göpel, W., Anderson, J.A., Frankel, D., Jaenig, M., Phillips, K., Achäafer, J.A., Rocker, G., (1984) Surf. Sci., 139, p. 333; Ancarz, I.G., Pozniak, G., Bryjak, M., Tylus, W., (2002) Eur. Polym. J., 38, p. 1937; Ratner, B.D., (1993) J. Biomed. Mater. Res., 27, p. 837; Park, J.Y., Gemmell, C.H., Davies, J.E., (2001) Biomaterials, 22, p. 2671; Maitz, M.F., Pham, M.T., Wieser, E., Tsyganov, I., (2003) J. Biomater. Appl., 17, p. 303; Sunny, M.C., Sharma, C.P., (1991) J. Biomater, Appl., 6, p. 89; Mustafa, K., Wroblewski, J., Hultenby, K., Silva Lopez, B., Arvidson, K., (2000) Clin. Oral Implant Res., 2, p. 116; Puleo, D.A., Kissling, R.A., Sheu, M.S., (2002) Biomaterials, 23, p. 2079; Qombotz, W.R., Huffman, A.S., (1988) J. Appl. Polym. Sci. Appl. Polym. Symp., 42, p. 285

PY - 2005/12/8

Y1 - 2005/12/8

N2 - The surface of implantable biomaterials directly contacts the host tissue and is critical in determining biocompatibility. To improve implant integration, interfacial reactions must be controlled to minimize nonspecific adsorption of proteins, and tissue-healing phenomena can be controlled. The purpose of this study was to develop a new method of functionalizing titanium surfaces by plasma treatment. The covalent immobilization of bioactive organic molecules and the bioactivities in vitro were assessed by transmission electron microscopy (TEM), atomic force spectroscopy (AFM), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), and 3-(4,5-dimethylthiazole-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay as indices of cellular cytotoxicity. Argon plasma removed all of the adsorbed contaminants and impurities. Plasma-cleaned titanium surfaces showed better bioactive performances than untreated titanium surfaces. The analytical results reveal that plasma-cleaned titanium surfaces provide a clean and reproducible starting condition for further plasma treatments to create well-controlled surface layers. Allylamine was ionized by plasma treatment, and acted as a medium to link albumin. Cells demonstrated a good spread, and a wide attachment was attained on the Albu-Ti plate. Cell attachment and growth were shown to be influenced by the surface properties. The plasma treatment process plays an important role in facilitating tissue healing. This process not only provides a clean titanium surface, but also leads to surface amination on plasma-treated titanium surfaces. Surface cleaning by ion bombardment and surface modification by plasma polymerization are believed to remove contamination on titanium surfaces and thus promote tissue healing.

AB - The surface of implantable biomaterials directly contacts the host tissue and is critical in determining biocompatibility. To improve implant integration, interfacial reactions must be controlled to minimize nonspecific adsorption of proteins, and tissue-healing phenomena can be controlled. The purpose of this study was to develop a new method of functionalizing titanium surfaces by plasma treatment. The covalent immobilization of bioactive organic molecules and the bioactivities in vitro were assessed by transmission electron microscopy (TEM), atomic force spectroscopy (AFM), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), and 3-(4,5-dimethylthiazole-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay as indices of cellular cytotoxicity. Argon plasma removed all of the adsorbed contaminants and impurities. Plasma-cleaned titanium surfaces showed better bioactive performances than untreated titanium surfaces. The analytical results reveal that plasma-cleaned titanium surfaces provide a clean and reproducible starting condition for further plasma treatments to create well-controlled surface layers. Allylamine was ionized by plasma treatment, and acted as a medium to link albumin. Cells demonstrated a good spread, and a wide attachment was attained on the Albu-Ti plate. Cell attachment and growth were shown to be influenced by the surface properties. The plasma treatment process plays an important role in facilitating tissue healing. This process not only provides a clean titanium surface, but also leads to surface amination on plasma-treated titanium surfaces. Surface cleaning by ion bombardment and surface modification by plasma polymerization are believed to remove contamination on titanium surfaces and thus promote tissue healing.

KW - Albumin

KW - Allyamine

KW - Biocompatibility

KW - Plasma polymerization

KW - Tissue healing

KW - Titanium

KW - Adsorption

KW - Atomic force microscopy

KW - Biomaterials

KW - Bromine compounds

KW - Cell immobilization

KW - Cytology

KW - Impurities

KW - Plasma applications

KW - Proteins

KW - Scanning electron microscopy

KW - Tissue

KW - Toxicity

KW - Transmission electron microscopy

KW - X ray photoelectron spectroscopy

KW - Plasma treatment

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JO - Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes

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