Enhanced expression of glycine N-methyltransferase by adenovirus-mediated gene transfer in CNS culture is neuroprotective

May Jywan Tsai, Yi Ming Arthur Chen, Ching Feng Weng, Dann Ying Liou, Hsin Chun Yang, Chien Hung Chen, Roanna I.Hsin Liao, Fu Shan Kuo, Chiuan Wen Chiu, Huai Sheng Kuo, Ming Chao Huang, Yi Lo Lin, Meng Jen Lee, Wen Chun Kuo, Wen Cheng Huang, Henrich Cheng

研究成果: 書貢獻/報告類型會議貢獻

8 引文 斯高帕斯(Scopus)

摘要

Glycine N-methyltransferase (GNMT) is the most abundant hepatic methyltransferase and plays important roles in regulating methyl group metabolism. In the central nervous system, GNMT expression is low and its function has not been revealed. The present study examines the effect of GNMT overexpression by adenovirus-mediated transfer in cortical mixed neuron-glial cultures. Infection of adenovirus encoding green fluorescence protein to cultures demonstrates high preference for non-neuronal cells. Optimal GNMT overexpression in cultures by adenoviral GNMT (Ad-GNMT) infection not only induces protein kinase C phosphorylation, but also increases neuronaloligodendroglial survival. Furthermore, these Ad-GNMT-infected cultures are significantly resistant to H2O2 toxicity and lipopolysaccharide stimulation. Conditioned media from Ad-GNMT-infected microglia also significantly enhance neuronal survival. Taken together, enhanced GNMT expression in mixed neuronal-glial cultures is neuroprotective, most likely mediated through non-neuronal cells.
原文英語
主出版物標題Neuroprotective Agents
主出版物子標題Ninth International Conference
發行者Blackwell Publishing Inc.
頁面194-203
頁數10
ISBN(列印)9781573317771
DOIs
出版狀態已發佈 - 一月 1 2010
對外發佈Yes

出版系列

名字Annals of the New York Academy of Sciences
1199
ISSN(列印)0077-8923
ISSN(電子)1749-6632

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

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    Tsai, M. J., Chen, Y. M. A., Weng, C. F., Liou, D. Y., Yang, H. C., Chen, C. H., Liao, R. I. H., Kuo, F. S., Chiu, C. W., Kuo, H. S., Huang, M. C., Lin, Y. L., Lee, M. J., Kuo, W. C., Huang, W. C., & Cheng, H. (2010). Enhanced expression of glycine N-methyltransferase by adenovirus-mediated gene transfer in CNS culture is neuroprotective. 於 Neuroprotective Agents: Ninth International Conference (頁 194-203). (Annals of the New York Academy of Sciences; 卷 1199). Blackwell Publishing Inc.. https://doi.org/10.1111/j.1749-6632.2009.05169.x