Endotoxin contamination contributes to the in vitro cytokine-inducing activity of osteopontin preparations

Satoshi Konno, Takuya Hoshi, Toshio Taira, Beverly Plunkett, Shau Ku Huang

研究成果: 雜誌貢獻文章同行評審

13 引文 斯高帕斯(Scopus)

摘要

Recently, both native and recombinant preparations of human osteopontin (OPN) have been shown to be able to induce the production of several proinflammatory cytokines in human peripheral blood mononuclear cells (PBMCs) or purified monocytes. In the present study, we found that commercially available native and recombinant OPNs contain variable amounts of endotoxin (LPS) and that removal of endotoxin by polymyxin B-agarose column abrogated their cytokine-inducing activity. These results suggest the questionable evidence of the ability of OPN to induce several cytokines in human PBMCs and draw attention to the exquisite sensitivity of PBMCs/monocytes to endotoxin contaminants.

原文英語
頁(從 - 到)277-282
頁數6
期刊Journal of Interferon and Cytokine Research
25
發行號5
DOIs
出版狀態已發佈 - 5月 1 2005
對外發佈

ASJC Scopus subject areas

  • 免疫學
  • 細胞生物學
  • 病毒學

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