Endothelin receptors in human and guinea-pig gallbladder muscle

Shih Che Huang, Ming Che Lee, Chang Kao Wei, Shih Ming Huang

研究成果: 雜誌貢獻文章同行評審

24 引文 斯高帕斯(Scopus)


We measured contraction of muscle strips caused by endothelin (ET) isopeptides and binding of 125I-ET-1 to muscle cell membranes prepared from human and guinea-pig gallbladders. Visualization of 125I-ET-1 binding sites in tissue was performed by autoradiography. Results in human were similar to those in guinea-pig. ET-1 caused tetrodotoxin and atropine-insensitive contraction. The relative potencies for ET isopeptides to cause contraction were ET-1 = ET-2 > ET-3. ET-1 caused contraction was only slightly inhibited by BQ-123 (potent ETA receptor antagonist) and not by BQ-788 (potent ETB receptor antagonist). It was inhibited by the combination of both. Autoradiography localized 125I-ET-1 binding to the smooth muscle layer. Binding of 125I-ET-1 to muscle cell membranes was saturable and specific. Analysis of dose-inhibition curves demonstrated the presence of two classes of receptors. One class (ETA receptor) had a high affinity for ET-1 and ET-2 but a low affinity for ET-3, and the other (ETB receptor) a high affinity for ET-1, ET-2 and ET-3. These results demonstrate that similar to guinea-pig, human gallbladder possesses both ETA and ETB receptors cooperating to mediate muscle contraction.

頁(從 - 到)145-153
期刊Regulatory Peptides
出版狀態已發佈 - 4月 20 2001

ASJC Scopus subject areas

  • 生物化學
  • 生理學
  • 內分泌
  • 臨床生物化學
  • 細胞與分子神經科學


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