Endothelial Cell Proliferation Assays

Wen Sen Lee

研究成果: 書貢獻/報告類型章節

4 引文 斯高帕斯(Scopus)


Angiogenesis is the generation of new blood vessels which occurs when a vascular bed is extended, such as in tissue growth, wound healing and tumour formation. In experimental studies, a useful, indirect estimation of angiogenesis activity is obtained by assessing the intensity of proliferation of the endothelial cell component of vascular tissue. Such endothelial cells are often isolated and maintained using common cell culture procedures, so that the effects of various mitogens (growth promoters) on cellular proliferation may be observed in vitro. A number of techniques for assessing cell proliferation are now in common use. In this chapter, we describe the basic methods and relevant principles. The most frequently used in vitro model for studying endothelial cell proliferation employs cultured human umbilical vein endothelial cells (HUVEC), which can be easily obtained. Each of the methods entails some advantages and some intrinsic weaknesses. To achieve the most reliable results, the combined use of two or more methods is to be recommended.

主出版物標題Angiogenesis Assays: A Critical Appraisal of Current Techniques
發行者John Wiley & Sons, Ltd
ISBN(列印)0470016000, 9780470016008
出版狀態已發佈 - 9月 27 2007

ASJC Scopus subject areas

  • 生物化學、遺傳與分子生物學 (全部)


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