Employing two distinct photonic crystal resonances to improve fluorescence enhancement

Patrick C. Mathias, Hsin Yu Wu, Brian T. Cunningham

研究成果: 雜誌貢獻文章同行評審

47 引文 斯高帕斯(Scopus)

摘要

Surface-bound fluorescence assays such as microarrays have emerged as a prominent technology in current life sciences research and are currently performed on optically passive substrates such as glass microscope slides. We present an alternative approach using photonic crystal substrates exhibiting resonant reflections. In this work, we design and fabricate a photonic crystal with a TM-polarized resonance at the cyanine-5 excitation wavelength and a TE-polarized resonance spectrally overlapping this fluorophore's emission spectrum. The former resonance increases the excitation of the fluorophore through enhanced electric field intensities, while the latter resonance redirects a proportion of emitted light toward the detection instrumentation. Spots of cyanine-5 conjugated streptavidin on the photonic crystal demonstrate a 60-fold increase in fluorescence intensity and a 42-fold increase in signal-to-noise ratio relative to a glass slide.

原文英語
文章編號021111
期刊Applied Physics Letters
95
發行號2
DOIs
出版狀態已發佈 - 2009

ASJC Scopus subject areas

  • 物理與天文學(雜項)

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