Emi2 Is Essential for Mouse Spermatogenesis

Lakshmi Gopinathan, Radoslaw Szmyd, Diana Low, M. Kasim Diril, Heng Yu Chang, Vincenzo Coppola, Kui Liu, Lino Tessarollo, Ernesto Guccione, Ans M.M. van Pelt, Philipp Kaldis

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13 引文 斯高帕斯(Scopus)

摘要

The meiotic functions of Emi2, an inhibitor of the APC/C complex, have been best characterized in oocytes where it mediates metaphase II arrest as a component of the cytostatic factor. We generated knockout mice to determine the in vivo functions of Emi2—in particular, its functions in the testis, where Emi2 is expressed at high levels. Male and female Emi2 knockout mice are viable but sterile, indicating that Emi2 is essential for meiosis but dispensable for embryonic development and mitotic cell divisions. We found that, besides regulating cell-cycle arrest in mouse eggs, Emi2 is essential for meiosis I progression in spermatocytes. In the absence of Emi2, spermatocytes arrest in early diplotene of prophase I. This arrest is associated with decreased Cdk1 activity and was partially rescued by a knockin mouse model of elevated Cdk1 activity. Additionally, we detected expression of Emi2 in spermatids and sperm, suggesting potential post-meiotic functions for Emi2.

原文英語
頁(從 - 到)697-708
頁數12
期刊Cell Reports
20
發行號3
DOIs
出版狀態已發佈 - 七月 18 2017

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Gopinathan, L., Szmyd, R., Low, D., Diril, M. K., Chang, H. Y., Coppola, V., Liu, K., Tessarollo, L., Guccione, E., van Pelt, A. M. M., & Kaldis, P. (2017). Emi2 Is Essential for Mouse Spermatogenesis. Cell Reports, 20(3), 697-708. https://doi.org/10.1016/j.celrep.2017.06.033