Ellagitannins from Terminalia calamansanai induced apoptosis in HL-60 cells

Lih Geeng Chen, Wen Tsung Huang, Lain Tze Lee, Ching Chiung Wang

研究成果: 雜誌貢獻文章同行評審

31 引文 斯高帕斯(Scopus)

摘要

Terminalia calamansanai (Blanco) Rolf. (Combretaceae) is used medicinally as lithontriptic in Philippines. The 70% acetone extracts of T. calamansanai leaves inhibited the viability of human promyelocytic leukemia HL-60 cells. 1-α-O-Galloylpunicalagin, punicalagin, 2-O-galloylpunicalin, sanguiin H-4, and methyl gallate were the main components isolated from T. calamansanai with the IC 50 values of 65.2, 74.8, 42.2, 38.0 and >100 μM, respectively, for HL-60 cells. Apoptosis of HL-60 cells treated with 1-α-O-galloylpunicalagin, punicalagin, 2-O-galloylpunicalin, and sanguiin H-4 was noted by the appearance of a sub-G 1 peak in flow cytometric analysis and DNA fragmentation by gel electrophoresis. 2-O-Galloylpunicalin and sanguiin H-4 induced a decrease of the human poly(ADP-ribose)polymerase (PARP) cleavage-related procaspase-3 and elevated activity of caspase-3 in HL-60 cells, but not normal human peripheral blood mononuclear cells (PBMCs), suggesting that both compounds may be new candidates for drug development in the prevention and treatment of cancer.

原文英語
頁(從 - 到)603-609
頁數7
期刊Toxicology in Vitro
23
發行號4
DOIs
出版狀態已發佈 - 六月 2009

ASJC Scopus subject areas

  • 毒理學

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