Elimination of cancer stem-like side population cells in hepatoma cell lines by Chinese herbal mixture Tien-Hsien Liquid

Chih Jung Yao, Chi-Tai Yeh, Liang Ming Lee, Shuang En Chuang, Chuan Feng Yeh, Wan Ju Chao, Tung-Iuan Lai, Gi Ming Lai

研究成果: 雜誌貢獻文章

16 引文 斯高帕斯(Scopus)

摘要

There are increasing pieces of evidence suggesting that the recurrence of cancer may result from a small subpopulation of cancer stem cells, which are resistant to the conventional chemotherapy and radiotherapy. We investigated the effects of Chinese herbal mixture Tien-Hsien Liquid (THL) on the cancer stem-like side population (SP) cells isolated from human hepatoma cells. After sorting and subsequent culture, the SP cells from Huh7 hepatoma cells appear to have higher clonogenicity and mRNA expressions of stemness genes such as SMO, ABCG2, CD133, β-catenin, and Oct-4 than those of non-SP cells. At dose of 2 mg/mL, THL reduced the proportion of SP cells in HepG2, Hep3B, and Huh7 cells from 1.33 to 0.49, 1.55 to 0.43, and 1.69 to 0.27, respectively. The viability and colony formation of Huh7 SP cells were effectively suppressed by THL dose-dependently, accompanied with the inhibition of stemness genes, e.g., ABCG2, CD133, and SMO. The tumorigenicity of THL-treated Huh7 SP cells in NOD/SCID mice was also diminished. Moreover, combination with THL could synergize the effect of doxorubicin against Huh7 SP cells. Our data indicate that THL may act as a cancer stem cell targeting therapeutics and be regarded as complementary and integrative medicine in the treatment of hepatoma.
原文英語
文章編號617085
期刊Evidence-based Complementary and Alternative Medicine
2012
DOIs
出版狀態已發佈 - 2012

ASJC Scopus subject areas

  • Complementary and alternative medicine

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