摘要
| 原文 | 英語 |
|---|---|
| 文章編號 | 617085 |
| 期刊 | Evidence-based Complementary and Alternative Medicine |
| 卷 | 2012 |
| DOIs | |
| 出版狀態 | 已發佈 - 2012 |
指紋
ASJC Scopus subject areas
- Complementary and alternative medicine
引用此文
Elimination of cancer stem-like side population cells in hepatoma cell lines by Chinese herbal mixture Tien-Hsien Liquid. / Yao, Chih Jung; Yeh, Chi-Tai; Lee, Liang Ming; Chuang, Shuang En; Yeh, Chuan Feng; Chao, Wan Ju; Lai, Tung-Iuan; Lai, Gi Ming.
於: Evidence-based Complementary and Alternative Medicine, 卷 2012, 617085, 2012.研究成果: 雜誌貢獻 › 文章
}
TY - JOUR
T1 - Elimination of cancer stem-like side population cells in hepatoma cell lines by Chinese herbal mixture Tien-Hsien Liquid
AU - Yao, Chih Jung
AU - Yeh, Chi-Tai
AU - Lee, Liang Ming
AU - Chuang, Shuang En
AU - Yeh, Chuan Feng
AU - Chao, Wan Ju
AU - Lai, Tung-Iuan
AU - Lai, Gi Ming
PY - 2012
Y1 - 2012
N2 - There are increasing pieces of evidence suggesting that the recurrence of cancer may result from a small subpopulation of cancer stem cells, which are resistant to the conventional chemotherapy and radiotherapy. We investigated the effects of Chinese herbal mixture Tien-Hsien Liquid (THL) on the cancer stem-like side population (SP) cells isolated from human hepatoma cells. After sorting and subsequent culture, the SP cells from Huh7 hepatoma cells appear to have higher clonogenicity and mRNA expressions of stemness genes such as SMO, ABCG2, CD133, β-catenin, and Oct-4 than those of non-SP cells. At dose of 2 mg/mL, THL reduced the proportion of SP cells in HepG2, Hep3B, and Huh7 cells from 1.33 to 0.49, 1.55 to 0.43, and 1.69 to 0.27, respectively. The viability and colony formation of Huh7 SP cells were effectively suppressed by THL dose-dependently, accompanied with the inhibition of stemness genes, e.g., ABCG2, CD133, and SMO. The tumorigenicity of THL-treated Huh7 SP cells in NOD/SCID mice was also diminished. Moreover, combination with THL could synergize the effect of doxorubicin against Huh7 SP cells. Our data indicate that THL may act as a cancer stem cell targeting therapeutics and be regarded as complementary and integrative medicine in the treatment of hepatoma.
AB - There are increasing pieces of evidence suggesting that the recurrence of cancer may result from a small subpopulation of cancer stem cells, which are resistant to the conventional chemotherapy and radiotherapy. We investigated the effects of Chinese herbal mixture Tien-Hsien Liquid (THL) on the cancer stem-like side population (SP) cells isolated from human hepatoma cells. After sorting and subsequent culture, the SP cells from Huh7 hepatoma cells appear to have higher clonogenicity and mRNA expressions of stemness genes such as SMO, ABCG2, CD133, β-catenin, and Oct-4 than those of non-SP cells. At dose of 2 mg/mL, THL reduced the proportion of SP cells in HepG2, Hep3B, and Huh7 cells from 1.33 to 0.49, 1.55 to 0.43, and 1.69 to 0.27, respectively. The viability and colony formation of Huh7 SP cells were effectively suppressed by THL dose-dependently, accompanied with the inhibition of stemness genes, e.g., ABCG2, CD133, and SMO. The tumorigenicity of THL-treated Huh7 SP cells in NOD/SCID mice was also diminished. Moreover, combination with THL could synergize the effect of doxorubicin against Huh7 SP cells. Our data indicate that THL may act as a cancer stem cell targeting therapeutics and be regarded as complementary and integrative medicine in the treatment of hepatoma.
UR - http://www.scopus.com/inward/record.url?scp=84868687454&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84868687454&partnerID=8YFLogxK
U2 - 10.1155/2012/617085
DO - 10.1155/2012/617085
M3 - Article
C2 - 23097677
AN - SCOPUS:84868687454
VL - 2012
JO - Evidence-based Complementary and Alternative Medicine
JF - Evidence-based Complementary and Alternative Medicine
SN - 1741-427X
M1 - 617085
ER -