Elevated galanin receptor type 2 primarily contributes to mechanical hypersensitivity after median nerve injury

Seu Hwa Chen, June Horng Lue, Yung Jung Hsiao, Shu Mei Lai, Hsin Ying Wang, Chi Te Lin, Ya Chin Chen, Yi Ju Tsai

研究成果: 雜誌貢獻文章

1 引文 (Scopus)

摘要

In this study, we investigated temporal changes in galanin receptor type 2 (GalR2) expression in NF200-, galanin-, neuropeptide Y (NPY)-, and neuronal nitric oxide synthase (nNOS)-like immunoreactive (LI) dorsal root ganglion (DRG) neurons after median nerve chronic constriction injury (CCI), and the effects of GalR2 on c-Fos expression in the cuneate nucleus (CN). Double immunofluorescence labeling methods were used to appraise changes in GalR2 expression in NF200-LI, galanin-LI, NPY-LI, and nNOS-LI DRG neurons after CCI. The von Frey assay was used to assess the efficiency of intraplantar administration of saline, M871 (a GalR2 antagonist), or AR-M1896 (a GalR2 agonist) on neuropathic signs of rats with CCI. The effects of alterations in c-Fos expression were assessed in all treatments. The percentage of GalR2-LI neurons in lesioned DRGs increased and peaked at 1 week after CCI. We further detected that percentages of GalR2-LI neurons labeled for NF200, galanin, NPY, and nNOS significantly increased following CCI. Furthermore, M871 remarkably attenuated tactile allodynia, but the sensation was slightly aggravated by AR-M1896 after CCI. Consequentially, after electrical stimulation of the CCI-treated median nerve, the number of c-Fos-LI neurons in the cuneate nucleus (CN) was significantly reduced in the M871 group, whereas it increased in the AR-M1896 group. These results suggest that activation of GalR2, probably through NPY or nitric oxide, induces c-Fos expression in the CN and transmits mechanical allodynia sensations to the thalamus.
原文英語
文章編號e0199512
期刊PLoS One
13
發行號6
DOIs
出版狀態已發佈 - 六月 1 2018

指紋

Receptor, Galanin, Type 2
Median Nerve
Constriction
hypersensitivity
Hypersensitivity
nerve tissue
neuropeptide Y
Neurons
Neuropeptide Y
receptors
Galanin
Wounds and Injuries
neurons
Nitric Oxide Synthase Type I
Hyperalgesia
Spinal Ganglia
thalamus
Diagnosis-Related Groups
Thalamus
Labeling

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

引用此文

Elevated galanin receptor type 2 primarily contributes to mechanical hypersensitivity after median nerve injury. / Chen, Seu Hwa; Lue, June Horng; Hsiao, Yung Jung; Lai, Shu Mei; Wang, Hsin Ying; Lin, Chi Te; Chen, Ya Chin; Tsai, Yi Ju.

於: PLoS One, 卷 13, 編號 6, e0199512, 01.06.2018.

研究成果: 雜誌貢獻文章

Chen, Seu Hwa ; Lue, June Horng ; Hsiao, Yung Jung ; Lai, Shu Mei ; Wang, Hsin Ying ; Lin, Chi Te ; Chen, Ya Chin ; Tsai, Yi Ju. / Elevated galanin receptor type 2 primarily contributes to mechanical hypersensitivity after median nerve injury. 於: PLoS One. 2018 ; 卷 13, 編號 6.
@article{e8d0da4224f149759ba37d3d0cd6f9fa,
title = "Elevated galanin receptor type 2 primarily contributes to mechanical hypersensitivity after median nerve injury",
abstract = "In this study, we investigated temporal changes in galanin receptor type 2 (GalR2) expression in NF200-, galanin-, neuropeptide Y (NPY)-, and neuronal nitric oxide synthase (nNOS)-like immunoreactive (LI) dorsal root ganglion (DRG) neurons after median nerve chronic constriction injury (CCI), and the effects of GalR2 on c-Fos expression in the cuneate nucleus (CN). Double immunofluorescence labeling methods were used to appraise changes in GalR2 expression in NF200-LI, galanin-LI, NPY-LI, and nNOS-LI DRG neurons after CCI. The von Frey assay was used to assess the efficiency of intraplantar administration of saline, M871 (a GalR2 antagonist), or AR-M1896 (a GalR2 agonist) on neuropathic signs of rats with CCI. The effects of alterations in c-Fos expression were assessed in all treatments. The percentage of GalR2-LI neurons in lesioned DRGs increased and peaked at 1 week after CCI. We further detected that percentages of GalR2-LI neurons labeled for NF200, galanin, NPY, and nNOS significantly increased following CCI. Furthermore, M871 remarkably attenuated tactile allodynia, but the sensation was slightly aggravated by AR-M1896 after CCI. Consequentially, after electrical stimulation of the CCI-treated median nerve, the number of c-Fos-LI neurons in the cuneate nucleus (CN) was significantly reduced in the M871 group, whereas it increased in the AR-M1896 group. These results suggest that activation of GalR2, probably through NPY or nitric oxide, induces c-Fos expression in the CN and transmits mechanical allodynia sensations to the thalamus.",
author = "Chen, {Seu Hwa} and Lue, {June Horng} and Hsiao, {Yung Jung} and Lai, {Shu Mei} and Wang, {Hsin Ying} and Lin, {Chi Te} and Chen, {Ya Chin} and Tsai, {Yi Ju}",
year = "2018",
month = "6",
day = "1",
doi = "10.1371/journal.pone.0199512",
language = "English",
volume = "13",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "6",

}

TY - JOUR

T1 - Elevated galanin receptor type 2 primarily contributes to mechanical hypersensitivity after median nerve injury

AU - Chen, Seu Hwa

AU - Lue, June Horng

AU - Hsiao, Yung Jung

AU - Lai, Shu Mei

AU - Wang, Hsin Ying

AU - Lin, Chi Te

AU - Chen, Ya Chin

AU - Tsai, Yi Ju

PY - 2018/6/1

Y1 - 2018/6/1

N2 - In this study, we investigated temporal changes in galanin receptor type 2 (GalR2) expression in NF200-, galanin-, neuropeptide Y (NPY)-, and neuronal nitric oxide synthase (nNOS)-like immunoreactive (LI) dorsal root ganglion (DRG) neurons after median nerve chronic constriction injury (CCI), and the effects of GalR2 on c-Fos expression in the cuneate nucleus (CN). Double immunofluorescence labeling methods were used to appraise changes in GalR2 expression in NF200-LI, galanin-LI, NPY-LI, and nNOS-LI DRG neurons after CCI. The von Frey assay was used to assess the efficiency of intraplantar administration of saline, M871 (a GalR2 antagonist), or AR-M1896 (a GalR2 agonist) on neuropathic signs of rats with CCI. The effects of alterations in c-Fos expression were assessed in all treatments. The percentage of GalR2-LI neurons in lesioned DRGs increased and peaked at 1 week after CCI. We further detected that percentages of GalR2-LI neurons labeled for NF200, galanin, NPY, and nNOS significantly increased following CCI. Furthermore, M871 remarkably attenuated tactile allodynia, but the sensation was slightly aggravated by AR-M1896 after CCI. Consequentially, after electrical stimulation of the CCI-treated median nerve, the number of c-Fos-LI neurons in the cuneate nucleus (CN) was significantly reduced in the M871 group, whereas it increased in the AR-M1896 group. These results suggest that activation of GalR2, probably through NPY or nitric oxide, induces c-Fos expression in the CN and transmits mechanical allodynia sensations to the thalamus.

AB - In this study, we investigated temporal changes in galanin receptor type 2 (GalR2) expression in NF200-, galanin-, neuropeptide Y (NPY)-, and neuronal nitric oxide synthase (nNOS)-like immunoreactive (LI) dorsal root ganglion (DRG) neurons after median nerve chronic constriction injury (CCI), and the effects of GalR2 on c-Fos expression in the cuneate nucleus (CN). Double immunofluorescence labeling methods were used to appraise changes in GalR2 expression in NF200-LI, galanin-LI, NPY-LI, and nNOS-LI DRG neurons after CCI. The von Frey assay was used to assess the efficiency of intraplantar administration of saline, M871 (a GalR2 antagonist), or AR-M1896 (a GalR2 agonist) on neuropathic signs of rats with CCI. The effects of alterations in c-Fos expression were assessed in all treatments. The percentage of GalR2-LI neurons in lesioned DRGs increased and peaked at 1 week after CCI. We further detected that percentages of GalR2-LI neurons labeled for NF200, galanin, NPY, and nNOS significantly increased following CCI. Furthermore, M871 remarkably attenuated tactile allodynia, but the sensation was slightly aggravated by AR-M1896 after CCI. Consequentially, after electrical stimulation of the CCI-treated median nerve, the number of c-Fos-LI neurons in the cuneate nucleus (CN) was significantly reduced in the M871 group, whereas it increased in the AR-M1896 group. These results suggest that activation of GalR2, probably through NPY or nitric oxide, induces c-Fos expression in the CN and transmits mechanical allodynia sensations to the thalamus.

UR - http://www.scopus.com/inward/record.url?scp=85048854489&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85048854489&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0199512

DO - 10.1371/journal.pone.0199512

M3 - Article

VL - 13

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 6

M1 - e0199512

ER -