Electroporation for three commonly used yeast strains for two-hybrid screening experiments

Hsin Ying Lin, Sey-En Lin, Su Fang Chien, Ming Kai Chern

研究成果: 雜誌貢獻文章

4 引文 (Scopus)

摘要

The efficiency of transformation by electroporation has been known to be compromised by strain dependency. A high efficiency protocol is still lacking for distinct two-hybrid yeast strains of diverse genetic features. Here, we used 0.5 M lithium acetate (LiAc) and 50 mM Tris-HCl with 5 mM EDTA (pH 7.5), i.e., fivefold the standard concentrations, and voltage at 1.0 to develop a protocol which, for the first time, is able to effect an average efficiency of 1.84 × 106 transformants/μg DNA for three commonly used yeast strains committed to two-hybrid screening experiments.

原文英語
頁(從 - 到)117-119
頁數3
期刊Analytical Biochemistry
416
發行號1
DOIs
出版狀態已發佈 - 九月 1 2011

指紋

Electroporation
Yeast
Screening
Yeasts
Edetic Acid
Experiments
DNA
Electric potential
lithium acetate

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology

引用此文

Electroporation for three commonly used yeast strains for two-hybrid screening experiments. / Lin, Hsin Ying; Lin, Sey-En; Chien, Su Fang; Chern, Ming Kai.

於: Analytical Biochemistry, 卷 416, 編號 1, 01.09.2011, p. 117-119.

研究成果: 雜誌貢獻文章

Lin, Hsin Ying ; Lin, Sey-En ; Chien, Su Fang ; Chern, Ming Kai. / Electroporation for three commonly used yeast strains for two-hybrid screening experiments. 於: Analytical Biochemistry. 2011 ; 卷 416, 編號 1. 頁 117-119.
@article{e2a604b3619c4a52bb8b17bfda4bc780,
title = "Electroporation for three commonly used yeast strains for two-hybrid screening experiments",
abstract = "The efficiency of transformation by electroporation has been known to be compromised by strain dependency. A high efficiency protocol is still lacking for distinct two-hybrid yeast strains of diverse genetic features. Here, we used 0.5 M lithium acetate (LiAc) and 50 mM Tris-HCl with 5 mM EDTA (pH 7.5), i.e., fivefold the standard concentrations, and voltage at 1.0 to develop a protocol which, for the first time, is able to effect an average efficiency of 1.84 × 106 transformants/μg DNA for three commonly used yeast strains committed to two-hybrid screening experiments.",
author = "Lin, {Hsin Ying} and Sey-En Lin and Chien, {Su Fang} and Chern, {Ming Kai}",
year = "2011",
month = "9",
day = "1",
doi = "10.1016/j.ab.2011.04.038",
language = "English",
volume = "416",
pages = "117--119",
journal = "Analytical Biochemistry",
issn = "0003-2697",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Electroporation for three commonly used yeast strains for two-hybrid screening experiments

AU - Lin, Hsin Ying

AU - Lin, Sey-En

AU - Chien, Su Fang

AU - Chern, Ming Kai

PY - 2011/9/1

Y1 - 2011/9/1

N2 - The efficiency of transformation by electroporation has been known to be compromised by strain dependency. A high efficiency protocol is still lacking for distinct two-hybrid yeast strains of diverse genetic features. Here, we used 0.5 M lithium acetate (LiAc) and 50 mM Tris-HCl with 5 mM EDTA (pH 7.5), i.e., fivefold the standard concentrations, and voltage at 1.0 to develop a protocol which, for the first time, is able to effect an average efficiency of 1.84 × 106 transformants/μg DNA for three commonly used yeast strains committed to two-hybrid screening experiments.

AB - The efficiency of transformation by electroporation has been known to be compromised by strain dependency. A high efficiency protocol is still lacking for distinct two-hybrid yeast strains of diverse genetic features. Here, we used 0.5 M lithium acetate (LiAc) and 50 mM Tris-HCl with 5 mM EDTA (pH 7.5), i.e., fivefold the standard concentrations, and voltage at 1.0 to develop a protocol which, for the first time, is able to effect an average efficiency of 1.84 × 106 transformants/μg DNA for three commonly used yeast strains committed to two-hybrid screening experiments.

UR - http://www.scopus.com/inward/record.url?scp=79959768607&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79959768607&partnerID=8YFLogxK

U2 - 10.1016/j.ab.2011.04.038

DO - 10.1016/j.ab.2011.04.038

M3 - Article

C2 - 21601557

AN - SCOPUS:79959768607

VL - 416

SP - 117

EP - 119

JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

IS - 1

ER -