Electroelution of DNA and protein from polyacrylamide and agarose gels.

T. Chang, I. H. Tsai, W. C. Chang

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3 引文 斯高帕斯(Scopus)

摘要

An electroelution method is described for the recovery of DNA and protein from agarose or polyacrylamide gels. The samples to be electroeluted are compartmentalized in a modified microcentrifuge tube fitted with dialysis membranes. This procedure is simple, rapid, inexpensive and efficient. Within 30 min to 2 hrs, the recovery of the sample is nearly quantitative. DNA fragments recovered can be directly subjected to DNA sequence analysis or enzymatic reactions after ethanol precipitation. Proteins can also be recovered after separation by acrylamide gel in the presence or absence of detergents and be ready for further analysis.
原文英語
頁(從 - 到)687-691
頁數5
期刊Biochemistry International
15
發行號4
出版狀態已發佈 - 十月 1 1987
對外發佈Yes

ASJC Scopus subject areas

  • Biochemistry

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