Electrochemistry-enabled fabrication of orthogonal nanotopography and surface chemistry gradients for high-throughput screening

Lauren R. Clements, Peng Yuan Wang, Wei Bor Tsai, Helmut Thissen, Nicolas H. Voelcker

研究成果: 雜誌貢獻文章同行評審

35 引文 斯高帕斯(Scopus)

摘要

Gradient surfaces are emerging tools for investigating mammalian cell-surface interactions in high throughput. We demonstrate the electrochemical fabrication of an orthogonal gradient platform combining a porous silicon (pSi) pore size gradient with an orthogonal gradient of peptide ligand density. pSi gradients were fabricated via the anodic etching of a silicon wafer with pore sizes ranging from hundreds to tens of nanometers. A chemical gradient of ethyl-6-bromohexanoate was generated orthogonally to the pSi gradient via electrochemical attachment. Subsequent hydrolysis and activation of the chemical gradient allowed for the generation of a cyclic RGD gradient. Whilst mesenchymal stem cells (MSC) were shown to respond to both the topographical and chemical cues arising from the orthogonal gradient, the MSC's responded more strongly to changes in RGD density than to changes in pore size during short-term culture.
原文英語
頁(從 - 到)1480-1486
頁數7
期刊Lab on a Chip - Miniaturisation for Chemistry and Biology
12
發行號8
DOIs
出版狀態已發佈 - 4月 21 2012
對外發佈

ASJC Scopus subject areas

  • 生物化學
  • 化學 (全部)
  • 生物工程
  • 生物醫學工程

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