摘要
Aging is considered as a primary risk factor in the development of osteoarthritis (OA) which associated with mitochondrial dysfunction and oxidative stress. CDGSH iron sulfur domain 2 (Cisd2) deficiency causes mitochondrial dysfunction and drive premature aging. In the present study, thermosensitive chitosan-gelatin based hydrogel containing glutathione was developed as injectable drug delivery system for administration by minimal invasive surgery for the treatment of OA. Cisd2 deficiency (Cisd2−/−) mouse induced pluripotent stem cells-derived chondrocytes were established and characterized. The results suggested that 100 μM of glutathione may be an optimal concentration to treat Cisd2−/− chondrocytes without cytotoxicity. The developed hydrogel showed sustained release profile of the glutathione and could decrease the reactive oxygen species level. Post-treatment of glutathione-loaded hydrogel could rescue Cisd2−/− chondrocytes from oxidative damage via increasing catalase activity, down-regulation of inflammation, and decreasing apoptosis. These results suggest that thermosensitive glutathione-loaded hydrogel may be a potential antioxidant therapeutic strategy for treating Cisd2−/− chondrocytes in the near future.
原文 | 英語 |
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頁(從 - 到) | 17-27 |
頁數 | 11 |
期刊 | Carbohydrate Polymers |
卷 | 173 |
DOIs | |
出版狀態 | 已發佈 - 10月 1 2017 |
ASJC Scopus subject areas
- 有機化學
- 聚合物和塑料
- 材料化學