摘要
原文 | 英語 |
---|---|
頁(從 - 到) | 3631-3643 |
頁數 | 13 |
期刊 | International Journal of Nanomedicine |
卷 | 9 |
發行號 | 1 |
DOIs | |
出版狀態 | 已發佈 - 八月 2 2014 |
指紋
ASJC Scopus subject areas
- Biophysics
- Bioengineering
- Biomaterials
- Organic Chemistry
- Drug Discovery
- Medicine(all)
引用此文
Effects of size and surface of zinc oxide and aluminum-doped zinc oxide nanoparticles on cell viability inferred by proteomic analyses. / Pan, Chih Hong; Liu, Wen Te; Bien, Mauo Ying; Lin, I. Chan; Hsiao, Ta Chih; Ma, Chih Ming; Lai, Ching Huang; Chen, Mei Chieh; Chuang, Kai Jen; Chuang, Hsiao Chi.
於: International Journal of Nanomedicine, 卷 9, 編號 1, 02.08.2014, p. 3631-3643.研究成果: 雜誌貢獻 › 文章
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TY - JOUR
T1 - Effects of size and surface of zinc oxide and aluminum-doped zinc oxide nanoparticles on cell viability inferred by proteomic analyses
AU - Pan, Chih Hong
AU - Liu, Wen Te
AU - Bien, Mauo Ying
AU - Lin, I. Chan
AU - Hsiao, Ta Chih
AU - Ma, Chih Ming
AU - Lai, Ching Huang
AU - Chen, Mei Chieh
AU - Chuang, Kai Jen
AU - Chuang, Hsiao Chi
PY - 2014/8/2
Y1 - 2014/8/2
N2 - Although the health effects of zinc oxide nanoparticles (ZnONPs) on the respiratory system have been reported, the fate, potential toxicity, and mechanisms in biological cells of these particles, as related to particle size and surface characteristics, have not been well elucidated. To determine the physicochemical properties of ZnONPs that govern cytotoxicity, we investigated the effects of size, electronic properties, zinc concentration, and pH on cell viability using human alveolar-basal epithelial A549 cells as a model. We observed that a 2-hour or longer exposure to ZnONPs induced changes in cell viability. The alteration in cell viability was associated with the zeta potentials and pH values of the ZnONPs. Proteomic profiling of A549 exposed to ZnONPs for 2 and 4 hours was used to determine the biological mechanisms of ZnONP toxicity. p53-pathway activation was the core mechanism regulating cell viability in response to particle size. Activation of the Wnt and TGFβ signaling pathways was also important in the cellular response to ZnONPs of different sizes. The cadherin and Wnt signaling pathways were important cellular mechanisms triggered by surface differences. These results suggested that the size and surface characteristics of ZnONPs might play an important role in their observed cytotoxicity. This approach facilitates the design of more comprehensive systems for the evaluation of nanoparticles.
AB - Although the health effects of zinc oxide nanoparticles (ZnONPs) on the respiratory system have been reported, the fate, potential toxicity, and mechanisms in biological cells of these particles, as related to particle size and surface characteristics, have not been well elucidated. To determine the physicochemical properties of ZnONPs that govern cytotoxicity, we investigated the effects of size, electronic properties, zinc concentration, and pH on cell viability using human alveolar-basal epithelial A549 cells as a model. We observed that a 2-hour or longer exposure to ZnONPs induced changes in cell viability. The alteration in cell viability was associated with the zeta potentials and pH values of the ZnONPs. Proteomic profiling of A549 exposed to ZnONPs for 2 and 4 hours was used to determine the biological mechanisms of ZnONP toxicity. p53-pathway activation was the core mechanism regulating cell viability in response to particle size. Activation of the Wnt and TGFβ signaling pathways was also important in the cellular response to ZnONPs of different sizes. The cadherin and Wnt signaling pathways were important cellular mechanisms triggered by surface differences. These results suggested that the size and surface characteristics of ZnONPs might play an important role in their observed cytotoxicity. This approach facilitates the design of more comprehensive systems for the evaluation of nanoparticles.
KW - Aluminium-doped zinc oxide
KW - Nanoparticle
KW - pH
KW - Toxicology
KW - WNT pathway
KW - Zeta potential
UR - http://www.scopus.com/inward/record.url?scp=84905484430&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84905484430&partnerID=8YFLogxK
U2 - 10.2147/IJN.S66651
DO - 10.2147/IJN.S66651
M3 - Article
C2 - 25120361
AN - SCOPUS:84905484430
VL - 9
SP - 3631
EP - 3643
JO - International Journal of Nanomedicine
JF - International Journal of Nanomedicine
SN - 1176-9114
IS - 1
ER -