Effects of resveratrol, a grape polyphenol, on uterine contraction and Ca2+ mobilization in rats in vivo and in vitro

Shih Min Hsia, Kai Lee Wang, Paulus S. Wang

研究成果: 雜誌貢獻文章

24 引文 斯高帕斯(Scopus)

摘要

Dysmenorrhea is directly related to elevate prostaglandin F (PGF) levels. In Western medicine, this condition is treated using nonsteroidal antiinflammatory drugs. Because nonsteroidal antiinflammatory drugs produce many side effects, Chinese medicinal therapy is considered as a feasible alternative for treating dysmenorrhea. Many special physiological components used in Chinese medicine, such as resveratrol, have been isolated and identified. Resveratrol has many physiological functions, such as antioxidation and anticarcinogenic effects. However, the relationship between uterine smooth muscle contraction and resveratrol remains unknown. Here, we studied the in vitro and in vivo effects of resveratrol on uterine smooth muscle contraction. The uterus was separated from a female Sprague Dawley rat, and uterine smooth muscle contraction activity was measured and recorded. The results demonstrated that 1) resveratrol treatment inhibited PGF-, oxytocin-, acetylcholine-, and carbacholinduced uterine contractions in rats; 2) resveratrol inhibited uterine contractions stimulated by the Ca2+ channel activator (Bay K 8644) and depolarization in response to high K + (KCl); 3) resveratrol inhibited PGF-induced increases in the [Ca2+]i in human uterine smooth muscle cells; 4) resveratrol could mimicCa2+ channel blockers to block Ca2+ influx through voltage-operated Ca2+ channels inthe plasma membrane; and 5) resveratrol inhibited PGF-induced uterine contractions in rats in vivo. Resveratrol inhibited uterine contractions induced by PGF and high K+ in a concentration-dependent manner in vitro; furthermore, it inhibited Ca2+-dependent uterine contractions. Thus, resveratrol consistently suppressed the increases in intracellular Ca2+ concentrations ([Ca2+]i) induced by PGF and high K+ concentrations. It can be assumed that resveratrol probably inhibited uterine contraction by blocking external Ca2+ influx, leading to decreased [Ca2+]i. Therefore, resveratrol can be considered as a feasible alternative therapeutic agent for dysmenorrhea.

原文英語
頁(從 - 到)2090-2099
頁數10
期刊Endocrinology
152
發行號5
DOIs
出版狀態已發佈 - 五月 2011
對外發佈Yes

ASJC Scopus subject areas

  • Endocrinology

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