Effects of prophylactic administration of glutamine on CD4+ T cell polarization and kidney injury in mice with polymicrobial sepsis

Yu Chen Hou, Jin Ming Wu, Kuen Yuan Chen, Po Da Chen, Cing Syuan Lei, Sung Ling Yeh, Ming Tsan Lin

研究成果: 雜誌貢獻文章

1 引文 斯高帕斯(Scopus)

摘要

This study investigated the effects of glutamine (GLN) pretreatment on CD4+ T cell polarization and remote kidney injury in mice with gut-derived polymicrobial sepsis. Mice were randomly assigned to 3 groups: normal control fed with AIN-93G diet, and 2 sepsis groups provided with either AIN-93G-based diet or identical components except part of casein was replaced by GLN. Mice were given their respective diets for 2 weeks. Then, mice in the sepsis groups were performed with cecal ligation and puncture and were sacrificed at 72 h after the surgery. Blood, spleens and kidneys were collected for further examination. The results showed that sepsis resulted in decreased circulating and splenic total T lymphocyte and CD4+ T cell percentages, whereas interleukin (IL)-4-, and Foxp3-expressing CD4+ T cells percentages were upregulated. Compared to the sepsis control group, pretreatment with GLN maintained blood T and CD4+ T cells, reduced percentages of IL-4- and Foxp3-expressing CD4+ T cells. Also, a more pronounced activation and increased anti-apoptotic Bcl-2 gene expression of splenic CD4+ T cells were observed. Concomitant with the decreased plasma IL-6, keratinocyte-derived chemokine (KC) levels, the gene expression of KC, macrophage inflammatory protein-2 and renal injury biomarker Kim-1 were down-regulated when GLN was administered. These findings suggest that antecedent of GLN administration elicit a more-balanced blood T helper cell polarization, sustained T cell populations, prevented splenic CD4+ T cell apoptosis and attenuated kidney injury at late phase of polymicrobial sepsis. GLN may have benefits in subjects in risk of abdominal infection.

原文英語
期刊British Journal of Nutrition
DOIs
出版狀態已發佈 - 一月 1 2019

    指紋

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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