Effects of ozone on DNA single-strand breaks and 8-oxoguanine formation in A549 cells

Tsun Jen Cheng, Hsuan Peng Kao, Chang Chuan Chan, Wushou P. Chang

研究成果: 雜誌貢獻文章

43 引文 (Scopus)

摘要

Animal studies have demonstrated that ozone exposure can induce lung tumors. Recent epidemiological studies have also shown that increased ozone exposure is associated with a greater risk of lung cancer. This study used single-cell gel electrophoresis (the Comet assay) and flow cytometry to investigate DNA damage in A549 cells exposed to ozone levels below the current ambient standard. Cells were exposed to ozone at levels of 0, 60, 80, and 120ppb, and then DNA single-strand breaks and 8-oxoguanine levels were measured. Additionally, the formamidopyrimidine glycosylase (Fpg) repair enzyme was added to the Comet assay to enhance detection of oxidative damage. Vitamins C and E were also added to determine their inhibitory effects on ozone-induced 8-oxoguanine. Measurements of tail length, tail intensity, and tail moment of the Comet assay were shown to correlate with each other. However, tail moment appeared to be more sensitive than the other two indicators in detecting DNA single-strand breaks. Tail moments of cells exposed to 80 and 120ppb of ozone were significantly higher than those exposed to 0ppb (P

原文英語
頁(從 - 到)279-284
頁數6
期刊Environmental Research
93
發行號3
DOIs
出版狀態已發佈 - 十一月 2003
對外發佈Yes

指紋

Single-Stranded DNA Breaks
Ozone
Comet Assay
ozone
DNA
comet
Assays
assay
damage
Flow cytometry
flow cytometry
ascorbic acid
Electrophoresis
Vitamin E
tumor
Ascorbic Acid
DNA Damage
repair
8-hydroxyguanine
A549 Cells

ASJC Scopus subject areas

  • Environmental Science(all)

引用此文

Effects of ozone on DNA single-strand breaks and 8-oxoguanine formation in A549 cells. / Cheng, Tsun Jen; Kao, Hsuan Peng; Chan, Chang Chuan; Chang, Wushou P.

於: Environmental Research, 卷 93, 編號 3, 11.2003, p. 279-284.

研究成果: 雜誌貢獻文章

Cheng, Tsun Jen ; Kao, Hsuan Peng ; Chan, Chang Chuan ; Chang, Wushou P. / Effects of ozone on DNA single-strand breaks and 8-oxoguanine formation in A549 cells. 於: Environmental Research. 2003 ; 卷 93, 編號 3. 頁 279-284.
@article{61da0a7a042a4e7d8b5acafdd8206c32,
title = "Effects of ozone on DNA single-strand breaks and 8-oxoguanine formation in A549 cells",
abstract = "Animal studies have demonstrated that ozone exposure can induce lung tumors. Recent epidemiological studies have also shown that increased ozone exposure is associated with a greater risk of lung cancer. This study used single-cell gel electrophoresis (the Comet assay) and flow cytometry to investigate DNA damage in A549 cells exposed to ozone levels below the current ambient standard. Cells were exposed to ozone at levels of 0, 60, 80, and 120ppb, and then DNA single-strand breaks and 8-oxoguanine levels were measured. Additionally, the formamidopyrimidine glycosylase (Fpg) repair enzyme was added to the Comet assay to enhance detection of oxidative damage. Vitamins C and E were also added to determine their inhibitory effects on ozone-induced 8-oxoguanine. Measurements of tail length, tail intensity, and tail moment of the Comet assay were shown to correlate with each other. However, tail moment appeared to be more sensitive than the other two indicators in detecting DNA single-strand breaks. Tail moments of cells exposed to 80 and 120ppb of ozone were significantly higher than those exposed to 0ppb (P",
keywords = "8-oxoguanine, Comet assay, Formamidopyrimidine glycosylase, Ozone, Vitamins C and E",
author = "Cheng, {Tsun Jen} and Kao, {Hsuan Peng} and Chan, {Chang Chuan} and Chang, {Wushou P.}",
year = "2003",
month = "11",
doi = "10.1016/S0013-9351(03)00041-0",
language = "English",
volume = "93",
pages = "279--284",
journal = "Environmental Research",
issn = "0013-9351",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Effects of ozone on DNA single-strand breaks and 8-oxoguanine formation in A549 cells

AU - Cheng, Tsun Jen

AU - Kao, Hsuan Peng

AU - Chan, Chang Chuan

AU - Chang, Wushou P.

PY - 2003/11

Y1 - 2003/11

N2 - Animal studies have demonstrated that ozone exposure can induce lung tumors. Recent epidemiological studies have also shown that increased ozone exposure is associated with a greater risk of lung cancer. This study used single-cell gel electrophoresis (the Comet assay) and flow cytometry to investigate DNA damage in A549 cells exposed to ozone levels below the current ambient standard. Cells were exposed to ozone at levels of 0, 60, 80, and 120ppb, and then DNA single-strand breaks and 8-oxoguanine levels were measured. Additionally, the formamidopyrimidine glycosylase (Fpg) repair enzyme was added to the Comet assay to enhance detection of oxidative damage. Vitamins C and E were also added to determine their inhibitory effects on ozone-induced 8-oxoguanine. Measurements of tail length, tail intensity, and tail moment of the Comet assay were shown to correlate with each other. However, tail moment appeared to be more sensitive than the other two indicators in detecting DNA single-strand breaks. Tail moments of cells exposed to 80 and 120ppb of ozone were significantly higher than those exposed to 0ppb (P

AB - Animal studies have demonstrated that ozone exposure can induce lung tumors. Recent epidemiological studies have also shown that increased ozone exposure is associated with a greater risk of lung cancer. This study used single-cell gel electrophoresis (the Comet assay) and flow cytometry to investigate DNA damage in A549 cells exposed to ozone levels below the current ambient standard. Cells were exposed to ozone at levels of 0, 60, 80, and 120ppb, and then DNA single-strand breaks and 8-oxoguanine levels were measured. Additionally, the formamidopyrimidine glycosylase (Fpg) repair enzyme was added to the Comet assay to enhance detection of oxidative damage. Vitamins C and E were also added to determine their inhibitory effects on ozone-induced 8-oxoguanine. Measurements of tail length, tail intensity, and tail moment of the Comet assay were shown to correlate with each other. However, tail moment appeared to be more sensitive than the other two indicators in detecting DNA single-strand breaks. Tail moments of cells exposed to 80 and 120ppb of ozone were significantly higher than those exposed to 0ppb (P

KW - 8-oxoguanine

KW - Comet assay

KW - Formamidopyrimidine glycosylase

KW - Ozone

KW - Vitamins C and E

UR - http://www.scopus.com/inward/record.url?scp=0242522256&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0242522256&partnerID=8YFLogxK

U2 - 10.1016/S0013-9351(03)00041-0

DO - 10.1016/S0013-9351(03)00041-0

M3 - Article

C2 - 14615238

AN - SCOPUS:0242522256

VL - 93

SP - 279

EP - 284

JO - Environmental Research

JF - Environmental Research

SN - 0013-9351

IS - 3

ER -