Effects of mineral trioxide aggregate and bioceramics on macrophage differentiation and polarization in vitro

Ming Gene Tu, Kuo Ting Sun, Tong Hong Wang, Yun Zhen He, Shih Min Hsia, Bi He Tsai, Yin Hwa Shih, Tzong Ming Shieh

研究成果: 雜誌貢獻文章

1 引文 (Scopus)

摘要

Background/Purpose: Mineral trioxide aggregate (Pro-Root MTA, PR-MTA) and bioceramics (iRoot® SP Injectable Root Canal Sealer, iR-BC) are used for making apical plugs used in apexification, repairing root perforations during root canal therapy, and treating internal root resorption. The purpose of the present in vitro study was to compare the biological effects of PR-MTA- and iR-BC-based dental sealers in the mouse macrophage cell line RAW 264.7. Methods: Cytotoxicity and cell proliferation were analyzed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and cell hemocytometer, respectively. Protein expression of biomarkers of cell proliferation, autophagy, and osteoclast differentiation was determined by western blotting. Pro-inflammatory gene expression was examined using quantitative reverse transcription-PCR. Results: PR-MTA induced cytotoxicity in RAW 264.7 cells in a dose-dependent manner, and iR-BC was more cytotoxic than PR-MTA. Low-dose and short-term treatments of both PR-MTA and iR-BC induced RAW 264.7 cell proliferation. PR-MTA induced autophagy, whereas iR-BC did not. Neither PR-MTA nor iR-BC induced osteoclastogenesis. Pro-inflammatory genes were activated by both materials. However, the expression of inducible nitric oxide synthase (iNOS) mRNA was upregulated by iR-BC treatment, but not by PR-MTA treatment. Conclusion: Overall, dental PR-MTA and iR-BC induced pro-inflammatory genes but did not induce osteoclastogenesis in macrophages. PR-MTA and iR-BC induced M2 and M1 polarization, respectively, of RAW 264.7 cells.

原文英語
期刊Journal of the Formosan Medical Association
DOIs
出版狀態已發佈 - 一月 1 2019

指紋

Pemetrexed
Macrophages
Autophagy
Cell Proliferation
Osteogenesis
Apexification
Root Canal Therapy
mineral trioxide aggregate
In Vitro Techniques
Root Resorption
Tooth Root
Dental Pulp Cavity
Nitric Oxide Synthase Type II
Osteoclasts
Genes
Reverse Transcription

ASJC Scopus subject areas

  • Medicine(all)

引用此文

Effects of mineral trioxide aggregate and bioceramics on macrophage differentiation and polarization in vitro. / Tu, Ming Gene; Sun, Kuo Ting; Wang, Tong Hong; He, Yun Zhen; Hsia, Shih Min; Tsai, Bi He; Shih, Yin Hwa; Shieh, Tzong Ming.

於: Journal of the Formosan Medical Association, 01.01.2019.

研究成果: 雜誌貢獻文章

Tu, Ming Gene ; Sun, Kuo Ting ; Wang, Tong Hong ; He, Yun Zhen ; Hsia, Shih Min ; Tsai, Bi He ; Shih, Yin Hwa ; Shieh, Tzong Ming. / Effects of mineral trioxide aggregate and bioceramics on macrophage differentiation and polarization in vitro. 於: Journal of the Formosan Medical Association. 2019.
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title = "Effects of mineral trioxide aggregate and bioceramics on macrophage differentiation and polarization in vitro",
abstract = "Background/Purpose: Mineral trioxide aggregate (Pro-Root MTA, PR-MTA) and bioceramics (iRoot{\circledR} SP Injectable Root Canal Sealer, iR-BC) are used for making apical plugs used in apexification, repairing root perforations during root canal therapy, and treating internal root resorption. The purpose of the present in vitro study was to compare the biological effects of PR-MTA- and iR-BC-based dental sealers in the mouse macrophage cell line RAW 264.7. Methods: Cytotoxicity and cell proliferation were analyzed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and cell hemocytometer, respectively. Protein expression of biomarkers of cell proliferation, autophagy, and osteoclast differentiation was determined by western blotting. Pro-inflammatory gene expression was examined using quantitative reverse transcription-PCR. Results: PR-MTA induced cytotoxicity in RAW 264.7 cells in a dose-dependent manner, and iR-BC was more cytotoxic than PR-MTA. Low-dose and short-term treatments of both PR-MTA and iR-BC induced RAW 264.7 cell proliferation. PR-MTA induced autophagy, whereas iR-BC did not. Neither PR-MTA nor iR-BC induced osteoclastogenesis. Pro-inflammatory genes were activated by both materials. However, the expression of inducible nitric oxide synthase (iNOS) mRNA was upregulated by iR-BC treatment, but not by PR-MTA treatment. Conclusion: Overall, dental PR-MTA and iR-BC induced pro-inflammatory genes but did not induce osteoclastogenesis in macrophages. PR-MTA and iR-BC induced M2 and M1 polarization, respectively, of RAW 264.7 cells.",
keywords = "Bioceramics (BC), Macrophage, Mineral trioxide aggregate (MTA), Osteoclastogenesis, Polarization",
author = "Tu, {Ming Gene} and Sun, {Kuo Ting} and Wang, {Tong Hong} and He, {Yun Zhen} and Hsia, {Shih Min} and Tsai, {Bi He} and Shih, {Yin Hwa} and Shieh, {Tzong Ming}",
year = "2019",
month = "1",
day = "1",
doi = "10.1016/j.jfma.2019.07.010",
language = "English",
journal = "Journal of the Formosan Medical Association",
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TY - JOUR

T1 - Effects of mineral trioxide aggregate and bioceramics on macrophage differentiation and polarization in vitro

AU - Tu, Ming Gene

AU - Sun, Kuo Ting

AU - Wang, Tong Hong

AU - He, Yun Zhen

AU - Hsia, Shih Min

AU - Tsai, Bi He

AU - Shih, Yin Hwa

AU - Shieh, Tzong Ming

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Background/Purpose: Mineral trioxide aggregate (Pro-Root MTA, PR-MTA) and bioceramics (iRoot® SP Injectable Root Canal Sealer, iR-BC) are used for making apical plugs used in apexification, repairing root perforations during root canal therapy, and treating internal root resorption. The purpose of the present in vitro study was to compare the biological effects of PR-MTA- and iR-BC-based dental sealers in the mouse macrophage cell line RAW 264.7. Methods: Cytotoxicity and cell proliferation were analyzed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and cell hemocytometer, respectively. Protein expression of biomarkers of cell proliferation, autophagy, and osteoclast differentiation was determined by western blotting. Pro-inflammatory gene expression was examined using quantitative reverse transcription-PCR. Results: PR-MTA induced cytotoxicity in RAW 264.7 cells in a dose-dependent manner, and iR-BC was more cytotoxic than PR-MTA. Low-dose and short-term treatments of both PR-MTA and iR-BC induced RAW 264.7 cell proliferation. PR-MTA induced autophagy, whereas iR-BC did not. Neither PR-MTA nor iR-BC induced osteoclastogenesis. Pro-inflammatory genes were activated by both materials. However, the expression of inducible nitric oxide synthase (iNOS) mRNA was upregulated by iR-BC treatment, but not by PR-MTA treatment. Conclusion: Overall, dental PR-MTA and iR-BC induced pro-inflammatory genes but did not induce osteoclastogenesis in macrophages. PR-MTA and iR-BC induced M2 and M1 polarization, respectively, of RAW 264.7 cells.

AB - Background/Purpose: Mineral trioxide aggregate (Pro-Root MTA, PR-MTA) and bioceramics (iRoot® SP Injectable Root Canal Sealer, iR-BC) are used for making apical plugs used in apexification, repairing root perforations during root canal therapy, and treating internal root resorption. The purpose of the present in vitro study was to compare the biological effects of PR-MTA- and iR-BC-based dental sealers in the mouse macrophage cell line RAW 264.7. Methods: Cytotoxicity and cell proliferation were analyzed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and cell hemocytometer, respectively. Protein expression of biomarkers of cell proliferation, autophagy, and osteoclast differentiation was determined by western blotting. Pro-inflammatory gene expression was examined using quantitative reverse transcription-PCR. Results: PR-MTA induced cytotoxicity in RAW 264.7 cells in a dose-dependent manner, and iR-BC was more cytotoxic than PR-MTA. Low-dose and short-term treatments of both PR-MTA and iR-BC induced RAW 264.7 cell proliferation. PR-MTA induced autophagy, whereas iR-BC did not. Neither PR-MTA nor iR-BC induced osteoclastogenesis. Pro-inflammatory genes were activated by both materials. However, the expression of inducible nitric oxide synthase (iNOS) mRNA was upregulated by iR-BC treatment, but not by PR-MTA treatment. Conclusion: Overall, dental PR-MTA and iR-BC induced pro-inflammatory genes but did not induce osteoclastogenesis in macrophages. PR-MTA and iR-BC induced M2 and M1 polarization, respectively, of RAW 264.7 cells.

KW - Bioceramics (BC)

KW - Macrophage

KW - Mineral trioxide aggregate (MTA)

KW - Osteoclastogenesis

KW - Polarization

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U2 - 10.1016/j.jfma.2019.07.010

DO - 10.1016/j.jfma.2019.07.010

M3 - Article

JO - Journal of the Formosan Medical Association

JF - Journal of the Formosan Medical Association

SN - 0929-6646

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