Effects of metallothionein-1 genetic polymorphism and cigarette smoking on the development of hepatocellular carcinoma

Ruey Hong Wong, Chun Huang Huang, Chao Bin Yeh, Hong Shen Lee, Ming Hsien Chien, Shun Fa Yang

研究成果: 雜誌貢獻文章

16 引文 (Scopus)

摘要

Background: A low expression of metallothionein (MT) has been observed in liver cancer. Such a phenomenon might be influenced by oxidative stress, thus resulting in the cells being more susceptible to DNA damage and apoptotic death. In particular, oxidative stress induced by cigarette smoking might affect MT-1 expression. We designed a hospital-based case-control study to evaluate the effects of MT-1 genotypes and smoking on hepatocellular carcinoma (HCC) occurrence. Methods: A total of 102 HCC patients and 191 matched healthy control subjects were recruited, and epidemiological information was collected. Six genotypes of MT-1 were determined with TaqMan single-nucleotide polymorphism genotyping assays. Results: Individuals possessing MT-1 rs8052394 A, rs964372 G, and rs8052334 T alleles as well as engaging in cigarette smoking had increased risks of HCC; these alleles also had higher linkage disequilibrium. Carriers with MT-1 rs8052394, rs964372, and rs8052334 A-G-T haplotype had a 2.25-fold (95 % confidence interval [CI] 1.46-3.26) risk for HCC development than the control group (A-C-T, the most common haplotype). Compared to nonsmokers with other haplotypes (A-C-T, G-G-C, A-G-C, G-G-T, G-C-T, and G-C-C), nonsmokers with A-G-T haplotype had a 1.93-fold (95 % CI 1.01-3.71) increased risk, and smokers with other haplotypes had a 3.66-fold (95 % CI 1.78-7.54) increased risk, whereas smokers carrying the A-G-T haplotype had the highest risk (matched relative risk 6.72; 95 % CI 2.86-15.79) of developing HCC. Conclusions: The MT-1 A-G-T haplotypes are associated with increased risk of HCC, especially in those who smoke.

原文英語
頁(從 - 到)2088-2095
頁數8
期刊Annals of Surgical Oncology
20
發行號6
DOIs
出版狀態已發佈 - 六月 2013

指紋

Metallothionein
Genetic Polymorphisms
Haplotypes
Hepatocellular Carcinoma
Smoking
Confidence Intervals
Oxidative Stress
Alleles
Genotype
Linkage Disequilibrium
Liver Neoplasms
Smoke
DNA Damage
Single Nucleotide Polymorphism
Case-Control Studies
Healthy Volunteers
Control Groups

ASJC Scopus subject areas

  • Surgery
  • Oncology

引用此文

Effects of metallothionein-1 genetic polymorphism and cigarette smoking on the development of hepatocellular carcinoma. / Wong, Ruey Hong; Huang, Chun Huang; Yeh, Chao Bin; Lee, Hong Shen; Chien, Ming Hsien; Yang, Shun Fa.

於: Annals of Surgical Oncology, 卷 20, 編號 6, 06.2013, p. 2088-2095.

研究成果: 雜誌貢獻文章

Wong, Ruey Hong ; Huang, Chun Huang ; Yeh, Chao Bin ; Lee, Hong Shen ; Chien, Ming Hsien ; Yang, Shun Fa. / Effects of metallothionein-1 genetic polymorphism and cigarette smoking on the development of hepatocellular carcinoma. 於: Annals of Surgical Oncology. 2013 ; 卷 20, 編號 6. 頁 2088-2095.
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title = "Effects of metallothionein-1 genetic polymorphism and cigarette smoking on the development of hepatocellular carcinoma",
abstract = "Background: A low expression of metallothionein (MT) has been observed in liver cancer. Such a phenomenon might be influenced by oxidative stress, thus resulting in the cells being more susceptible to DNA damage and apoptotic death. In particular, oxidative stress induced by cigarette smoking might affect MT-1 expression. We designed a hospital-based case-control study to evaluate the effects of MT-1 genotypes and smoking on hepatocellular carcinoma (HCC) occurrence. Methods: A total of 102 HCC patients and 191 matched healthy control subjects were recruited, and epidemiological information was collected. Six genotypes of MT-1 were determined with TaqMan single-nucleotide polymorphism genotyping assays. Results: Individuals possessing MT-1 rs8052394 A, rs964372 G, and rs8052334 T alleles as well as engaging in cigarette smoking had increased risks of HCC; these alleles also had higher linkage disequilibrium. Carriers with MT-1 rs8052394, rs964372, and rs8052334 A-G-T haplotype had a 2.25-fold (95 {\%} confidence interval [CI] 1.46-3.26) risk for HCC development than the control group (A-C-T, the most common haplotype). Compared to nonsmokers with other haplotypes (A-C-T, G-G-C, A-G-C, G-G-T, G-C-T, and G-C-C), nonsmokers with A-G-T haplotype had a 1.93-fold (95 {\%} CI 1.01-3.71) increased risk, and smokers with other haplotypes had a 3.66-fold (95 {\%} CI 1.78-7.54) increased risk, whereas smokers carrying the A-G-T haplotype had the highest risk (matched relative risk 6.72; 95 {\%} CI 2.86-15.79) of developing HCC. Conclusions: The MT-1 A-G-T haplotypes are associated with increased risk of HCC, especially in those who smoke.",
author = "Wong, {Ruey Hong} and Huang, {Chun Huang} and Yeh, {Chao Bin} and Lee, {Hong Shen} and Chien, {Ming Hsien} and Yang, {Shun Fa}",
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T1 - Effects of metallothionein-1 genetic polymorphism and cigarette smoking on the development of hepatocellular carcinoma

AU - Wong, Ruey Hong

AU - Huang, Chun Huang

AU - Yeh, Chao Bin

AU - Lee, Hong Shen

AU - Chien, Ming Hsien

AU - Yang, Shun Fa

PY - 2013/6

Y1 - 2013/6

N2 - Background: A low expression of metallothionein (MT) has been observed in liver cancer. Such a phenomenon might be influenced by oxidative stress, thus resulting in the cells being more susceptible to DNA damage and apoptotic death. In particular, oxidative stress induced by cigarette smoking might affect MT-1 expression. We designed a hospital-based case-control study to evaluate the effects of MT-1 genotypes and smoking on hepatocellular carcinoma (HCC) occurrence. Methods: A total of 102 HCC patients and 191 matched healthy control subjects were recruited, and epidemiological information was collected. Six genotypes of MT-1 were determined with TaqMan single-nucleotide polymorphism genotyping assays. Results: Individuals possessing MT-1 rs8052394 A, rs964372 G, and rs8052334 T alleles as well as engaging in cigarette smoking had increased risks of HCC; these alleles also had higher linkage disequilibrium. Carriers with MT-1 rs8052394, rs964372, and rs8052334 A-G-T haplotype had a 2.25-fold (95 % confidence interval [CI] 1.46-3.26) risk for HCC development than the control group (A-C-T, the most common haplotype). Compared to nonsmokers with other haplotypes (A-C-T, G-G-C, A-G-C, G-G-T, G-C-T, and G-C-C), nonsmokers with A-G-T haplotype had a 1.93-fold (95 % CI 1.01-3.71) increased risk, and smokers with other haplotypes had a 3.66-fold (95 % CI 1.78-7.54) increased risk, whereas smokers carrying the A-G-T haplotype had the highest risk (matched relative risk 6.72; 95 % CI 2.86-15.79) of developing HCC. Conclusions: The MT-1 A-G-T haplotypes are associated with increased risk of HCC, especially in those who smoke.

AB - Background: A low expression of metallothionein (MT) has been observed in liver cancer. Such a phenomenon might be influenced by oxidative stress, thus resulting in the cells being more susceptible to DNA damage and apoptotic death. In particular, oxidative stress induced by cigarette smoking might affect MT-1 expression. We designed a hospital-based case-control study to evaluate the effects of MT-1 genotypes and smoking on hepatocellular carcinoma (HCC) occurrence. Methods: A total of 102 HCC patients and 191 matched healthy control subjects were recruited, and epidemiological information was collected. Six genotypes of MT-1 were determined with TaqMan single-nucleotide polymorphism genotyping assays. Results: Individuals possessing MT-1 rs8052394 A, rs964372 G, and rs8052334 T alleles as well as engaging in cigarette smoking had increased risks of HCC; these alleles also had higher linkage disequilibrium. Carriers with MT-1 rs8052394, rs964372, and rs8052334 A-G-T haplotype had a 2.25-fold (95 % confidence interval [CI] 1.46-3.26) risk for HCC development than the control group (A-C-T, the most common haplotype). Compared to nonsmokers with other haplotypes (A-C-T, G-G-C, A-G-C, G-G-T, G-C-T, and G-C-C), nonsmokers with A-G-T haplotype had a 1.93-fold (95 % CI 1.01-3.71) increased risk, and smokers with other haplotypes had a 3.66-fold (95 % CI 1.78-7.54) increased risk, whereas smokers carrying the A-G-T haplotype had the highest risk (matched relative risk 6.72; 95 % CI 2.86-15.79) of developing HCC. Conclusions: The MT-1 A-G-T haplotypes are associated with increased risk of HCC, especially in those who smoke.

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