Effects of low molecular weight chitosans on aristolochic acid-induced renal lesions in mice

Ya Min Chang, Chen Tien Chang, Tzu Chuan Huang, Shih Ming Chen, Jen Ai Lee, Yun Chin Chung

研究成果: 雜誌貢獻文章

5 引文 (Scopus)

摘要

This study investigated the effects of low molecular weight chitosan (LMWC) on the renal functions for aristolochic acid (AA)-induced renal lesions in C3H/He mice. Commercial crab shell chitosan (molecular mass of ∼1100 kDa) was hydrolysed using bamboo chitosanase at pH 3.5 and 50 °C for 18 h. LMWC (with molecular mass of ∼29 kDa) was isolated from the hydrolysate through 50% methanol fractionation. The levels of urinary microalbumin, urinary-N-acetyl-beta-d-glucosaminidase, serum creatinine, and blood urea nitrogen were higher in the AA induced mice than those in the control group, while the rate for creatinine clearance was lower in the AA induced mice. Oral administration of LMWC could suppress the elevation of urinary and blood nitrogen concentrations induced by AA treatment. Renal histopathological examination revealed that AA-induced renal alterations were reverted effectively by the treatment of LMWC at three tested doses, especially at the moderate dose of 500 mg/kg BW.

原文英語
頁(從 - 到)1751-1758
頁數8
期刊Food Chemistry
129
發行號4
DOIs
出版狀態已發佈 - 十二月 15 2011

指紋

Chitosan
chitosan
lesions (animal)
Molecular Weight
Molecular weight
kidneys
molecular weight
Kidney
acids
mice
chitosanase
Molecular mass
Creatinine
Blood
Nitrogen
creatinine
Hexosaminidases
Bamboo
Inbred C3H Mouse
Blood Urea Nitrogen

ASJC Scopus subject areas

  • Food Science
  • Analytical Chemistry

引用此文

Effects of low molecular weight chitosans on aristolochic acid-induced renal lesions in mice. / Chang, Ya Min; Chang, Chen Tien; Huang, Tzu Chuan; Chen, Shih Ming; Lee, Jen Ai; Chung, Yun Chin.

於: Food Chemistry, 卷 129, 編號 4, 15.12.2011, p. 1751-1758.

研究成果: 雜誌貢獻文章

Chang, Ya Min ; Chang, Chen Tien ; Huang, Tzu Chuan ; Chen, Shih Ming ; Lee, Jen Ai ; Chung, Yun Chin. / Effects of low molecular weight chitosans on aristolochic acid-induced renal lesions in mice. 於: Food Chemistry. 2011 ; 卷 129, 編號 4. 頁 1751-1758.
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abstract = "This study investigated the effects of low molecular weight chitosan (LMWC) on the renal functions for aristolochic acid (AA)-induced renal lesions in C3H/He mice. Commercial crab shell chitosan (molecular mass of ∼1100 kDa) was hydrolysed using bamboo chitosanase at pH 3.5 and 50 °C for 18 h. LMWC (with molecular mass of ∼29 kDa) was isolated from the hydrolysate through 50{\%} methanol fractionation. The levels of urinary microalbumin, urinary-N-acetyl-beta-d-glucosaminidase, serum creatinine, and blood urea nitrogen were higher in the AA induced mice than those in the control group, while the rate for creatinine clearance was lower in the AA induced mice. Oral administration of LMWC could suppress the elevation of urinary and blood nitrogen concentrations induced by AA treatment. Renal histopathological examination revealed that AA-induced renal alterations were reverted effectively by the treatment of LMWC at three tested doses, especially at the moderate dose of 500 mg/kg BW.",
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AU - Chang, Chen Tien

AU - Huang, Tzu Chuan

AU - Chen, Shih Ming

AU - Lee, Jen Ai

AU - Chung, Yun Chin

PY - 2011/12/15

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AB - This study investigated the effects of low molecular weight chitosan (LMWC) on the renal functions for aristolochic acid (AA)-induced renal lesions in C3H/He mice. Commercial crab shell chitosan (molecular mass of ∼1100 kDa) was hydrolysed using bamboo chitosanase at pH 3.5 and 50 °C for 18 h. LMWC (with molecular mass of ∼29 kDa) was isolated from the hydrolysate through 50% methanol fractionation. The levels of urinary microalbumin, urinary-N-acetyl-beta-d-glucosaminidase, serum creatinine, and blood urea nitrogen were higher in the AA induced mice than those in the control group, while the rate for creatinine clearance was lower in the AA induced mice. Oral administration of LMWC could suppress the elevation of urinary and blood nitrogen concentrations induced by AA treatment. Renal histopathological examination revealed that AA-induced renal alterations were reverted effectively by the treatment of LMWC at three tested doses, especially at the moderate dose of 500 mg/kg BW.

KW - Aristolochic acid

KW - Bamboo chitosanase

KW - Low molecular weight chitosans

KW - Renal histopathology

KW - Renal lesions

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