摘要
This study investigated the effects of low molecular weight chitosan (LMWC) on the renal functions for aristolochic acid (AA)-induced renal lesions in C3H/He mice. Commercial crab shell chitosan (molecular mass of ∼1100 kDa) was hydrolysed using bamboo chitosanase at pH 3.5 and 50 °C for 18 h. LMWC (with molecular mass of ∼29 kDa) was isolated from the hydrolysate through 50% methanol fractionation. The levels of urinary microalbumin, urinary-N-acetyl-beta-d-glucosaminidase, serum creatinine, and blood urea nitrogen were higher in the AA induced mice than those in the control group, while the rate for creatinine clearance was lower in the AA induced mice. Oral administration of LMWC could suppress the elevation of urinary and blood nitrogen concentrations induced by AA treatment. Renal histopathological examination revealed that AA-induced renal alterations were reverted effectively by the treatment of LMWC at three tested doses, especially at the moderate dose of 500 mg/kg BW.
| 原文 | 英語 |
|---|---|
| 頁（從 - 到） | 1751-1758 |
| 頁數 | 8 |
| 期刊 | Food Chemistry |
| 卷 | 129 |
| 發行號 | 4 |
| DOIs | |
| 出版狀態 | 已發佈 - 十二月 15 2011 |
指紋
ASJC Scopus subject areas
- Food Science
- Analytical Chemistry
引用此文
Effects of low molecular weight chitosans on aristolochic acid-induced renal lesions in mice. / Chang, Ya Min; Chang, Chen Tien; Huang, Tzu Chuan; Chen, Shih Ming; Lee, Jen Ai; Chung, Yun Chin.
於: Food Chemistry, 卷 129, 編號 4, 15.12.2011, p. 1751-1758.研究成果: 雜誌貢獻 › 文章
}
TY - JOUR
T1 - Effects of low molecular weight chitosans on aristolochic acid-induced renal lesions in mice
AU - Chang, Ya Min
AU - Chang, Chen Tien
AU - Huang, Tzu Chuan
AU - Chen, Shih Ming
AU - Lee, Jen Ai
AU - Chung, Yun Chin
PY - 2011/12/15
Y1 - 2011/12/15
N2 - This study investigated the effects of low molecular weight chitosan (LMWC) on the renal functions for aristolochic acid (AA)-induced renal lesions in C3H/He mice. Commercial crab shell chitosan (molecular mass of ∼1100 kDa) was hydrolysed using bamboo chitosanase at pH 3.5 and 50 °C for 18 h. LMWC (with molecular mass of ∼29 kDa) was isolated from the hydrolysate through 50% methanol fractionation. The levels of urinary microalbumin, urinary-N-acetyl-beta-d-glucosaminidase, serum creatinine, and blood urea nitrogen were higher in the AA induced mice than those in the control group, while the rate for creatinine clearance was lower in the AA induced mice. Oral administration of LMWC could suppress the elevation of urinary and blood nitrogen concentrations induced by AA treatment. Renal histopathological examination revealed that AA-induced renal alterations were reverted effectively by the treatment of LMWC at three tested doses, especially at the moderate dose of 500 mg/kg BW.
AB - This study investigated the effects of low molecular weight chitosan (LMWC) on the renal functions for aristolochic acid (AA)-induced renal lesions in C3H/He mice. Commercial crab shell chitosan (molecular mass of ∼1100 kDa) was hydrolysed using bamboo chitosanase at pH 3.5 and 50 °C for 18 h. LMWC (with molecular mass of ∼29 kDa) was isolated from the hydrolysate through 50% methanol fractionation. The levels of urinary microalbumin, urinary-N-acetyl-beta-d-glucosaminidase, serum creatinine, and blood urea nitrogen were higher in the AA induced mice than those in the control group, while the rate for creatinine clearance was lower in the AA induced mice. Oral administration of LMWC could suppress the elevation of urinary and blood nitrogen concentrations induced by AA treatment. Renal histopathological examination revealed that AA-induced renal alterations were reverted effectively by the treatment of LMWC at three tested doses, especially at the moderate dose of 500 mg/kg BW.
KW - Aristolochic acid
KW - Bamboo chitosanase
KW - Low molecular weight chitosans
KW - Renal histopathology
KW - Renal lesions
UR - http://www.scopus.com/inward/record.url?scp=80051791008&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80051791008&partnerID=8YFLogxK
U2 - 10.1016/j.foodchem.2011.06.044
DO - 10.1016/j.foodchem.2011.06.044
M3 - Article
AN - SCOPUS:80051791008
VL - 129
SP - 1751
EP - 1758
JO - Food Chemistry
JF - Food Chemistry
SN - 0308-8146
IS - 4
ER -