Effects of low-intensity pulsed ultrasound, dexamethasone/TGF-β1 and/or BMP-2 on the transcriptional expression of genes in human mesenchymal stem cells: Chondrogenic vs. osteogenic differentiation

Chien Hung Lai, Shih Ching Chen, Li Hsuan Chiu, Charng Bin Yang, Yu Hui Tsai, Chun S. Zuo, Walter Hong Shong Chang, Wen-FuThomas Lai

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44 引文 斯高帕斯(Scopus)

摘要

The effects of low-intensity pulsed ultrasound (LIPUS) on the differentiation of human mesenchymal stem cells (hMSCs) were investigated in this study. hMSCs were subjected to LIPUS with or without dexamethasone/transforming growth factor-β1 (TD) or bone morphogenetic protein-2 (BMP-2) and the effects of this treatment were assessed. TD-treated hMSCs exhibited characteristic chondrogenic morphology and increased messenger RNA (mRNA) expression of chondrogenic markers and LIPUS enhanced the chondrogenic differentiation of hMSCs treated with TD. The expression of Runx2, an osteogenic transcription factor was not altered in either TD treatment group; however, a significant increase was detected in the LIPUS only group. The osteogenic appearance exhibited 3 days after LIPUS and/or BMP-2 treatment. Increases in the mRNA expression levels of osteogenic markers, Runx2 and ALP were also detected. There was no additive or altered effect with combined LIPUS and BMP-2 treatment. LIPUS alone can increase osteogenic differentiation of hMSCs and LIPUS enhances TD-mediated chondrogenic differentiation of hMSCs. Clinically, LIPUS may differentially influence bone vs. cartilage repair.
原文英語
頁(從 - 到)1022-1033
頁數12
期刊Ultrasound in Medicine and Biology
36
發行號6
DOIs
出版狀態已發佈 - 2010

ASJC Scopus subject areas

  • 放射學、核子醫學和影像學
  • 放射與超音波技術
  • 生物物理學
  • 醫藥 (全部)

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