Effects of growth factors and granulosa cell co-culture on in-vitro maturation of oocytes

Yu Hung Lin, Jiann-Loung Hwang, Kok Min Seow, Lee Wen Huang, Heng Ju Chen, Chii Ruey Tzeng

研究成果: 雜誌貢獻文章同行評審

30 引文 斯高帕斯(Scopus)

摘要

The maturation medium for in-vitro oocyte maturation is usually supplemented with serum. However, supplementation with serum from pregnant women adversely affects the outcome of in-vitro maturation. The purpose of the study was to assess if growth factors or granulosa cell co-culture could overcome the adverse effects of pregnant women's serum. The basal maturation medium consisted of TCM199, 75 mIU/ml human menopausal gonadotrophin (HMG), 0.2 mmol/l pyruvate, and 10% serum. The maturation medium for control 1 contained fertile women's serum. The maturation medium for control 2 contained pregnant women's serum. The maturation media for the study groups consisted of medium for control 2, with the addition of EGF, IGF-I, activin, TGFβ or granulosa cell co-culture. Immature oocytes were obtained from FVB mice, and the experiment was repeated six times. After maturation, the oocytes were fertilized and cultured to blastocysts, and the cumulus cells were analysed for apoptosis. The maturation, fertilization and blastocyst rates of the control 2 group were significantly lower than those of control 1 group (P <0.05). Addition of EGF, IGF-1, activin, TGFβ or granulosa cell co-culture could not improve the outcome of in-vitro maturation. Cumulus cell proliferation was inhibited by pregnant women's serum. Apoptosis of cumulus cell was not related to in-vitro oocyte maturation and subsequent embryo development.
原文英語
頁(從 - 到)165-170
頁數6
期刊Reproductive BioMedicine Online
19
發行號2
DOIs
出版狀態已發佈 - 八月 2009

ASJC Scopus subject areas

  • 生殖醫學
  • 婦產科
  • 發展生物學

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