Effects of dietary glutamine on adhesion molecule expression and oxidative stress in mice with streptozotocin-induced type 1 diabetes

Pei Hsuan Tsai, Jun-Jen Liu, Wan-Chun Chiu, Man-Hui Pai, Sung-Ling Yeh

研究成果: 雜誌貢獻文章

18 引文 (Scopus)

摘要

Background & aims: Glutamine (Gln) is known to have immunomodulatory effects. Previous studies reported that Gln promotes insulin secretion in type 2 diabetes. However, the effects of Gln on insulin-deficient type 1 diabetes are not clear. This study investigated the effects of dietary Gln supplementation on adhesion molecule expression and oxidative damage in type 1 diabetic mice. Methods: There were 1 normal control (NC) group and 2 diabetic groups in this study. Mice in the NC group were fed a regular chow diet. One diabetic group (DM) was fed a common semipurified diet while the other diabetic group received a diet in which part of the casein was replaced by Gln (DM-Gln), which provided 25% of the total amino acid nitrogen for 6 wk. Diabetes was induced by an intraperitoneal injection of streptozotocin at a dose of 150 mg/kg body weight. Blood samples and the liver and kidneys of the animals were collected at the end of the study for further analysis. Results: Plasma glucose, fructosamine contents and adhesion molecule expressions were significantly higher in the diabetic groups than in the NC group. The DM group had higher leukocyte CD11a/CD18 expression. In diabetic mice, nitrotyrosine concentrations and myeloperoxidase activities were higher and the reduced to oxidized glutathione ratio was lower in liver and/or kidney. These alterations were not found in diabetic mice supplemented with Gln. Conclusions: These results suggest that supplemental dietary Gln increased the antioxidant potential and consequently decreased leukocyte adhesion molecule expression and oxidative stress in organs of mice with type 1 diabetes.
原文英語
頁(從 - 到)124-129
頁數6
期刊Clinical Nutrition
30
發行號1
DOIs
出版狀態已發佈 - 二月 2011

指紋

Streptozocin
Glutamine
Type 1 Diabetes Mellitus
Oxidative Stress
Diet
Control Groups
Insulin
Fructosamine
Kidney
Glutathione Disulfide
Liver
Cell Adhesion Molecules
Dietary Supplements
Caseins
Intraperitoneal Injections
Type 2 Diabetes Mellitus
Peroxidase
Leukocytes
Nitrogen
Antioxidants

Keywords

  • Adhesion molecule
  • Glutamine
  • Glutathione
  • Nitrotyrosine
  • Type 1 diabetes

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Nutrition and Dietetics

引用此文

@article{797f3e07a48a443a9cb3605be6db22e4,
title = "Effects of dietary glutamine on adhesion molecule expression and oxidative stress in mice with streptozotocin-induced type 1 diabetes",
abstract = "Background & aims: Glutamine (Gln) is known to have immunomodulatory effects. Previous studies reported that Gln promotes insulin secretion in type 2 diabetes. However, the effects of Gln on insulin-deficient type 1 diabetes are not clear. This study investigated the effects of dietary Gln supplementation on adhesion molecule expression and oxidative damage in type 1 diabetic mice. Methods: There were 1 normal control (NC) group and 2 diabetic groups in this study. Mice in the NC group were fed a regular chow diet. One diabetic group (DM) was fed a common semipurified diet while the other diabetic group received a diet in which part of the casein was replaced by Gln (DM-Gln), which provided 25{\%} of the total amino acid nitrogen for 6 wk. Diabetes was induced by an intraperitoneal injection of streptozotocin at a dose of 150 mg/kg body weight. Blood samples and the liver and kidneys of the animals were collected at the end of the study for further analysis. Results: Plasma glucose, fructosamine contents and adhesion molecule expressions were significantly higher in the diabetic groups than in the NC group. The DM group had higher leukocyte CD11a/CD18 expression. In diabetic mice, nitrotyrosine concentrations and myeloperoxidase activities were higher and the reduced to oxidized glutathione ratio was lower in liver and/or kidney. These alterations were not found in diabetic mice supplemented with Gln. Conclusions: These results suggest that supplemental dietary Gln increased the antioxidant potential and consequently decreased leukocyte adhesion molecule expression and oxidative stress in organs of mice with type 1 diabetes.",
keywords = "Adhesion molecule, Glutamine, Glutathione, Nitrotyrosine, Type 1 diabetes, Adhesion molecule, Glutamine, Glutathione, Nitrotyrosine, Type 1 diabetes",
author = "Tsai, {Pei Hsuan} and Jun-Jen Liu and Wan-Chun Chiu and Man-Hui Pai and Sung-Ling Yeh",
year = "2011",
month = "2",
doi = "10.1016/j.clnu.2010.07.005",
language = "English",
volume = "30",
pages = "124--129",
journal = "Clinical Nutrition",
issn = "0261-5614",
publisher = "Churchill Livingstone",
number = "1",

}

TY - JOUR

T1 - Effects of dietary glutamine on adhesion molecule expression and oxidative stress in mice with streptozotocin-induced type 1 diabetes

AU - Tsai, Pei Hsuan

AU - Liu, Jun-Jen

AU - Chiu, Wan-Chun

AU - Pai, Man-Hui

AU - Yeh, Sung-Ling

PY - 2011/2

Y1 - 2011/2

N2 - Background & aims: Glutamine (Gln) is known to have immunomodulatory effects. Previous studies reported that Gln promotes insulin secretion in type 2 diabetes. However, the effects of Gln on insulin-deficient type 1 diabetes are not clear. This study investigated the effects of dietary Gln supplementation on adhesion molecule expression and oxidative damage in type 1 diabetic mice. Methods: There were 1 normal control (NC) group and 2 diabetic groups in this study. Mice in the NC group were fed a regular chow diet. One diabetic group (DM) was fed a common semipurified diet while the other diabetic group received a diet in which part of the casein was replaced by Gln (DM-Gln), which provided 25% of the total amino acid nitrogen for 6 wk. Diabetes was induced by an intraperitoneal injection of streptozotocin at a dose of 150 mg/kg body weight. Blood samples and the liver and kidneys of the animals were collected at the end of the study for further analysis. Results: Plasma glucose, fructosamine contents and adhesion molecule expressions were significantly higher in the diabetic groups than in the NC group. The DM group had higher leukocyte CD11a/CD18 expression. In diabetic mice, nitrotyrosine concentrations and myeloperoxidase activities were higher and the reduced to oxidized glutathione ratio was lower in liver and/or kidney. These alterations were not found in diabetic mice supplemented with Gln. Conclusions: These results suggest that supplemental dietary Gln increased the antioxidant potential and consequently decreased leukocyte adhesion molecule expression and oxidative stress in organs of mice with type 1 diabetes.

AB - Background & aims: Glutamine (Gln) is known to have immunomodulatory effects. Previous studies reported that Gln promotes insulin secretion in type 2 diabetes. However, the effects of Gln on insulin-deficient type 1 diabetes are not clear. This study investigated the effects of dietary Gln supplementation on adhesion molecule expression and oxidative damage in type 1 diabetic mice. Methods: There were 1 normal control (NC) group and 2 diabetic groups in this study. Mice in the NC group were fed a regular chow diet. One diabetic group (DM) was fed a common semipurified diet while the other diabetic group received a diet in which part of the casein was replaced by Gln (DM-Gln), which provided 25% of the total amino acid nitrogen for 6 wk. Diabetes was induced by an intraperitoneal injection of streptozotocin at a dose of 150 mg/kg body weight. Blood samples and the liver and kidneys of the animals were collected at the end of the study for further analysis. Results: Plasma glucose, fructosamine contents and adhesion molecule expressions were significantly higher in the diabetic groups than in the NC group. The DM group had higher leukocyte CD11a/CD18 expression. In diabetic mice, nitrotyrosine concentrations and myeloperoxidase activities were higher and the reduced to oxidized glutathione ratio was lower in liver and/or kidney. These alterations were not found in diabetic mice supplemented with Gln. Conclusions: These results suggest that supplemental dietary Gln increased the antioxidant potential and consequently decreased leukocyte adhesion molecule expression and oxidative stress in organs of mice with type 1 diabetes.

KW - Adhesion molecule

KW - Glutamine

KW - Glutathione

KW - Nitrotyrosine

KW - Type 1 diabetes

KW - Adhesion molecule

KW - Glutamine

KW - Glutathione

KW - Nitrotyrosine

KW - Type 1 diabetes

UR - http://www.scopus.com/inward/record.url?scp=79451470232&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79451470232&partnerID=8YFLogxK

U2 - 10.1016/j.clnu.2010.07.005

DO - 10.1016/j.clnu.2010.07.005

M3 - Article

C2 - 20705374

AN - SCOPUS:79451470232

VL - 30

SP - 124

EP - 129

JO - Clinical Nutrition

JF - Clinical Nutrition

SN - 0261-5614

IS - 1

ER -