摘要
原文 | 英語 |
---|---|
頁(從 - 到) | 949-957 |
頁數 | 9 |
期刊 | Drug Design, Development and Therapy |
卷 | 10 |
DOIs | |
出版狀態 | 已發佈 - 三月 2 2016 |
指紋
ASJC Scopus subject areas
- Pharmaceutical Science
- Pharmacology
- Drug Discovery
引用此文
Effects of Dang-Gui-Bu-Xue-Tang, an herbal decoction, on iron uptake in iron-deficient anemia. / Huang, Guan Cheng; Chen, Shih Yu; Tsai, Po Wei; Ganzon, Jerome G.; Lee, Chia Jung; Shiah, Her Shyong; Wang, Ching Chiung.
於: Drug Design, Development and Therapy, 卷 10, 02.03.2016, p. 949-957.研究成果: 雜誌貢獻 › 文章
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TY - JOUR
T1 - Effects of Dang-Gui-Bu-Xue-Tang, an herbal decoction, on iron uptake in iron-deficient anemia
AU - Huang, Guan Cheng
AU - Chen, Shih Yu
AU - Tsai, Po Wei
AU - Ganzon, Jerome G.
AU - Lee, Chia Jung
AU - Shiah, Her Shyong
AU - Wang, Ching Chiung
PY - 2016/3/2
Y1 - 2016/3/2
N2 - Dang-Gui-Bu-Xue-Tang (DBT), a combination of Angelicae Sinensis Radix and Astragali Radix, is a widely used herbal decoction in traditional Chinese medicine primarily to promote or invigorate the “blood”. In this study, we explored this ancient formulation and provide evidence of its blood-toning properties. We used the improvement iron uptake as promote or invigorate the “blood” indicator. Ferritin formation of Caco-2 cells in vitro assay and diet-induced anemia (DIA) in rat model were used to prove its improvement iron uptake and ameliorating effects. Finally, the iron–DBT interactions were measured by iron-binding assay. We first demonstrated DBT increased uptake of ferrous iron through the biosynthesis of ferritin by Caco-2 cells and determined which complementary treatment would provide optimum results. Thereafter, effects of the treatment on improving the bioavailability of absorbed iron in the form of hemoglobin (Hb) were established using a DIA-animal model. The results showed that DBT slightly improved Hb levels compared with the baseline Hb and pretreatment with DBT for 2 hours prior to supplementation with ferrous sulfate provided the greatest gain in Hb levels in DIA rats. However, DBT and ferrous sulfate were co-treated with Caco-2 cell or DIA rats, the ferritin formation and Hb levels both were decreased. In iron-binding assay, the DBT extract influenced the free Fe(II) type in the FeSO4 solution. Therefore, we suggest that complementary treatment with DBT and iron supplementation can have a strong ameliorating effect on iron-deficiency anemia in clinical settings, but needs a 2-hour interval of DBT administration prior to ferrous sulfate treatment.
AB - Dang-Gui-Bu-Xue-Tang (DBT), a combination of Angelicae Sinensis Radix and Astragali Radix, is a widely used herbal decoction in traditional Chinese medicine primarily to promote or invigorate the “blood”. In this study, we explored this ancient formulation and provide evidence of its blood-toning properties. We used the improvement iron uptake as promote or invigorate the “blood” indicator. Ferritin formation of Caco-2 cells in vitro assay and diet-induced anemia (DIA) in rat model were used to prove its improvement iron uptake and ameliorating effects. Finally, the iron–DBT interactions were measured by iron-binding assay. We first demonstrated DBT increased uptake of ferrous iron through the biosynthesis of ferritin by Caco-2 cells and determined which complementary treatment would provide optimum results. Thereafter, effects of the treatment on improving the bioavailability of absorbed iron in the form of hemoglobin (Hb) were established using a DIA-animal model. The results showed that DBT slightly improved Hb levels compared with the baseline Hb and pretreatment with DBT for 2 hours prior to supplementation with ferrous sulfate provided the greatest gain in Hb levels in DIA rats. However, DBT and ferrous sulfate were co-treated with Caco-2 cell or DIA rats, the ferritin formation and Hb levels both were decreased. In iron-binding assay, the DBT extract influenced the free Fe(II) type in the FeSO4 solution. Therefore, we suggest that complementary treatment with DBT and iron supplementation can have a strong ameliorating effect on iron-deficiency anemia in clinical settings, but needs a 2-hour interval of DBT administration prior to ferrous sulfate treatment.
KW - Dang-Gui-Bu-Xue-Tang
KW - Ferritin
KW - Ferulic acid
KW - Herb–drug interaction
KW - Iron uptake
KW - Iron-deficiency anemia
UR - http://www.scopus.com/inward/record.url?scp=84959462812&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84959462812&partnerID=8YFLogxK
U2 - 10.2147/DDDT.S94309
DO - 10.2147/DDDT.S94309
M3 - Article
C2 - 27041997
AN - SCOPUS:84959462812
VL - 10
SP - 949
EP - 957
JO - Drug Design, Development and Therapy
JF - Drug Design, Development and Therapy
SN - 1177-8881
ER -