Effects of crude adlay hull acetone extract on corticosterone release from rat zona fasciculata-reticularis cells

Ling Ling Chang, Alfred Wan Song Wun, Chien Te Hung, Shih Min Hsia, Wenchang Chiang, Paulus S. Wang

研究成果: 雜誌貢獻文章

6 引文 (Scopus)

摘要

Adlay is a grass crop which has been used in traditional Chinese medicine and also as a nourishing food. It has been shown to posses anti-allergic, antimutagenic and hypolipemic effects. However, the effects and action mechanisms of crude adlay hull acetone extract (AHA) on adrenal zona fasciculata-reticularis (ZFR) cells are still unclear. This study explored the effects of AHA on corticosterone release. ZFR cells were incubated with AHA in the presence or absence of adrenocorticotropin (ACTH), 8-bromo-cyclic 3′: 5′- adenosine monophosphate (8-Br-cAMP), forskolin (FSK), 25-hydroxy cholesterol (25-OH-cholesterol), pregnenolone, progesterone or deoxycorticosterone. The concentrations of corticosterone or pregnenolone in the media were measured by radioimmunoassay (RIA). The cells were used to measure the expression of steroidogenic acute regulatory (StAR) protein by Western blot. The present data demonstrated that: (1) AHA inhibited ACTH-, 8-Br-cAMP-, forskolin-, 25-OH-cholesterol-, pregnenolone-, progesterone- or deoxycorticosterone-stimulated corticosterone release; (2) AHA (800 μg/ml) caused more pregnenolone release in control group, but not in 25-OH-cholesterol, trilostane or 25-OH-cholesterol+trilostane group; (3) kinetic study showed an uncompetitive inhibition model of AHA to P450 side chain cleavage enzyme (P450scc); (4) kinetic study showed a noncompetitive inhibition model of AHA to 11β-hydroxylase; and (5) AHA inhibited the expression of StAR protein. These results suggest that AHA acts directly upon rat ZFR cells to diminish corticosterone release. These results indicate the inhibitory mechanism of AHA mediates through an inhibition of the activities of the post-cAMP corticosterone synthesis enzymes, i.e. 3β-HSD, 21-hydroxylase, 11β-hydroxylase, and inhibition of StAR protein expression.

原文英語
頁(從 - 到)141-152
頁數12
期刊Naunyn-Schmiedeberg's Archives of Pharmacology
374
發行號2
DOIs
出版狀態已發佈 - 十一月 2006
對外發佈Yes

指紋

Zona Reticularis
Zona Fasciculata
Corticosterone
Acetone
Pregnenolone
Cholesterol
Adrenocorticotropic Hormone
Desoxycorticosterone
Colforsin
Adenosine Monophosphate
Mixed Function Oxygenases
Progesterone
Antimutagenic Agents
Steroid 21-Hydroxylase
Anti-Allergic Agents
Chinese Traditional Medicine
Enzymes
Poaceae
Radioimmunoassay
Western Blotting

ASJC Scopus subject areas

  • Pharmacology

引用此文

Effects of crude adlay hull acetone extract on corticosterone release from rat zona fasciculata-reticularis cells. / Chang, Ling Ling; Wun, Alfred Wan Song; Hung, Chien Te; Hsia, Shih Min; Chiang, Wenchang; Wang, Paulus S.

於: Naunyn-Schmiedeberg's Archives of Pharmacology, 卷 374, 編號 2, 11.2006, p. 141-152.

研究成果: 雜誌貢獻文章

Chang, Ling Ling ; Wun, Alfred Wan Song ; Hung, Chien Te ; Hsia, Shih Min ; Chiang, Wenchang ; Wang, Paulus S. / Effects of crude adlay hull acetone extract on corticosterone release from rat zona fasciculata-reticularis cells. 於: Naunyn-Schmiedeberg's Archives of Pharmacology. 2006 ; 卷 374, 編號 2. 頁 141-152.
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abstract = "Adlay is a grass crop which has been used in traditional Chinese medicine and also as a nourishing food. It has been shown to posses anti-allergic, antimutagenic and hypolipemic effects. However, the effects and action mechanisms of crude adlay hull acetone extract (AHA) on adrenal zona fasciculata-reticularis (ZFR) cells are still unclear. This study explored the effects of AHA on corticosterone release. ZFR cells were incubated with AHA in the presence or absence of adrenocorticotropin (ACTH), 8-bromo-cyclic 3′: 5′- adenosine monophosphate (8-Br-cAMP), forskolin (FSK), 25-hydroxy cholesterol (25-OH-cholesterol), pregnenolone, progesterone or deoxycorticosterone. The concentrations of corticosterone or pregnenolone in the media were measured by radioimmunoassay (RIA). The cells were used to measure the expression of steroidogenic acute regulatory (StAR) protein by Western blot. The present data demonstrated that: (1) AHA inhibited ACTH-, 8-Br-cAMP-, forskolin-, 25-OH-cholesterol-, pregnenolone-, progesterone- or deoxycorticosterone-stimulated corticosterone release; (2) AHA (800 μg/ml) caused more pregnenolone release in control group, but not in 25-OH-cholesterol, trilostane or 25-OH-cholesterol+trilostane group; (3) kinetic study showed an uncompetitive inhibition model of AHA to P450 side chain cleavage enzyme (P450scc); (4) kinetic study showed a noncompetitive inhibition model of AHA to 11β-hydroxylase; and (5) AHA inhibited the expression of StAR protein. These results suggest that AHA acts directly upon rat ZFR cells to diminish corticosterone release. These results indicate the inhibitory mechanism of AHA mediates through an inhibition of the activities of the post-cAMP corticosterone synthesis enzymes, i.e. 3β-HSD, 21-hydroxylase, 11β-hydroxylase, and inhibition of StAR protein expression.",
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AU - Chiang, Wenchang

AU - Wang, Paulus S.

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