Effects of Arginine Supplementation on Exogenous Advanced Glycation End Product-induced Renal Inflammatory Mediator Expression in Rats

Ya Mei Hu, Chiu Li Yeh, Man Hui Pai, Cheng Chung Li, Jun Jen Liu, Sung Ling Yeh

研究成果: 雜誌貢獻文章

摘要

Background: This study investigated the effects of dietary Arg supplementation on renal inflammatory mediator expression and oxidative damage in rats with exogenous advanced glycation end product (AGE) administration. Methods: There were two groups of rats: the CA group was fed a common diet and given a tail vein injection of AGE-bovine serum albumin (BSA); and the AA group was fed the Arg-supplemented diet and injected with AGE-BSA. Arg provided 2% of the total energy. The tail vein injection and diets for the respective groups were given for 10 days. After that, all rats were sacrificed, and blood and kidneys were harvested for further analysis. Results: Blood adhesion molecule expression and NO levels were higher in the AA group. Also, the kidney nitrotyrosine concentration, phospho-nuclear factor-κB p65 and inducible NO synthase protein expression was higher in the AA group than in the CA group. The finding of immunohistochemical staining was consistent with the results that the AA group had higher receptor of AGE expression in the kidneys. Conclusion: Supplemental dietary Arg may have adverse effect in AGE-induced kidney inflammatory response and oxidative damage in rats.
原文英語
頁(從 - 到)24-29
頁數6
期刊Journal of Experimental and Clinical Medicine(Taiwan)
4
發行號1
DOIs
出版狀態已發佈 - 二月 2012

指紋

Advanced Glycosylation End Products
Arginine
Kidney
Diet
Tail
Veins
Injections
Dietary Supplements
Nitric Oxide Synthase
Staining and Labeling
Proteins

Keywords

  • Advanced glycation end products
  • Arginine
  • Inducible NO synthase
  • Nitrotyrosine

ASJC Scopus subject areas

  • Medicine(all)

引用此文

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title = "Effects of Arginine Supplementation on Exogenous Advanced Glycation End Product-induced Renal Inflammatory Mediator Expression in Rats",
abstract = "Background: This study investigated the effects of dietary Arg supplementation on renal inflammatory mediator expression and oxidative damage in rats with exogenous advanced glycation end product (AGE) administration. Methods: There were two groups of rats: the CA group was fed a common diet and given a tail vein injection of AGE-bovine serum albumin (BSA); and the AA group was fed the Arg-supplemented diet and injected with AGE-BSA. Arg provided 2{\%} of the total energy. The tail vein injection and diets for the respective groups were given for 10 days. After that, all rats were sacrificed, and blood and kidneys were harvested for further analysis. Results: Blood adhesion molecule expression and NO levels were higher in the AA group. Also, the kidney nitrotyrosine concentration, phospho-nuclear factor-κB p65 and inducible NO synthase protein expression was higher in the AA group than in the CA group. The finding of immunohistochemical staining was consistent with the results that the AA group had higher receptor of AGE expression in the kidneys. Conclusion: Supplemental dietary Arg may have adverse effect in AGE-induced kidney inflammatory response and oxidative damage in rats.",
keywords = "Advanced glycation end products, Arginine, Inducible NO synthase, Nitrotyrosine, Advanced glycation end products, Arginine, Inducible NO synthase, Nitrotyrosine",
author = "Hu, {Ya Mei} and Yeh, {Chiu Li} and Pai, {Man Hui} and Li, {Cheng Chung} and Liu, {Jun Jen} and Yeh, {Sung Ling}",
year = "2012",
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T1 - Effects of Arginine Supplementation on Exogenous Advanced Glycation End Product-induced Renal Inflammatory Mediator Expression in Rats

AU - Hu, Ya Mei

AU - Yeh, Chiu Li

AU - Pai, Man Hui

AU - Li, Cheng Chung

AU - Liu, Jun Jen

AU - Yeh, Sung Ling

PY - 2012/2

Y1 - 2012/2

N2 - Background: This study investigated the effects of dietary Arg supplementation on renal inflammatory mediator expression and oxidative damage in rats with exogenous advanced glycation end product (AGE) administration. Methods: There were two groups of rats: the CA group was fed a common diet and given a tail vein injection of AGE-bovine serum albumin (BSA); and the AA group was fed the Arg-supplemented diet and injected with AGE-BSA. Arg provided 2% of the total energy. The tail vein injection and diets for the respective groups were given for 10 days. After that, all rats were sacrificed, and blood and kidneys were harvested for further analysis. Results: Blood adhesion molecule expression and NO levels were higher in the AA group. Also, the kidney nitrotyrosine concentration, phospho-nuclear factor-κB p65 and inducible NO synthase protein expression was higher in the AA group than in the CA group. The finding of immunohistochemical staining was consistent with the results that the AA group had higher receptor of AGE expression in the kidneys. Conclusion: Supplemental dietary Arg may have adverse effect in AGE-induced kidney inflammatory response and oxidative damage in rats.

AB - Background: This study investigated the effects of dietary Arg supplementation on renal inflammatory mediator expression and oxidative damage in rats with exogenous advanced glycation end product (AGE) administration. Methods: There were two groups of rats: the CA group was fed a common diet and given a tail vein injection of AGE-bovine serum albumin (BSA); and the AA group was fed the Arg-supplemented diet and injected with AGE-BSA. Arg provided 2% of the total energy. The tail vein injection and diets for the respective groups were given for 10 days. After that, all rats were sacrificed, and blood and kidneys were harvested for further analysis. Results: Blood adhesion molecule expression and NO levels were higher in the AA group. Also, the kidney nitrotyrosine concentration, phospho-nuclear factor-κB p65 and inducible NO synthase protein expression was higher in the AA group than in the CA group. The finding of immunohistochemical staining was consistent with the results that the AA group had higher receptor of AGE expression in the kidneys. Conclusion: Supplemental dietary Arg may have adverse effect in AGE-induced kidney inflammatory response and oxidative damage in rats.

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KW - Inducible NO synthase

KW - Nitrotyrosine

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