Effect of surfactant and budesonide on the pulmonary distribution of fluorescent dye in mice

Liang Ti Huang, Tsu Fu Yeh, Yu Lin Kuo, Pin Chuan Chen, Chung Ming Chen

研究成果: 雜誌貢獻文章同行評審

14 引文 斯高帕斯(Scopus)

摘要

BACKGROUND: Surfactant is a useful vehicle for the intratracheal delivery of medicine to the distal lung. The aim of this study was to analyze the effect of intratracheal surfactant and budesonide instillation on the pulmonary distribution of fluorescent dye in mice.

METHODS: Male athymic nude mice were assigned randomly as controls, fluorescent dye, fluorescent dye + surfactant (50 mg/kg), fluorescent dye + budesonide (0.25 mg/kg), and fluorescent dye + surfactant + budesonide groups. A total volume of 60 μL fluorescent solutions was intratracheally injected and followed by 60 μL of air. We photographed and measured fluorescence in the lungs, from the back, 15 minutes after intratracheal administration using an IVIS Xenogen imaging instrument.

RESULTS: The fluorescent dye (1,1'-dioctadecyltetramethyl indotricarbocyanine iodide) was most strongly detected near the trachea and weakly detected in the lungs in mice administered with fluorescent solutions. Almost no fluorescence was seen in the lung region of control mice. Intratracheal administration of surfactant or budesonide increased fluorescent intensity compared with control mice. Combined administration of surfactant and budesonide further increased fluorescent intensity compared with mice given surfactant or budesonide alone.

CONCLUSION: Surfactant and budesonide enhance the pulmonary distribution of fluorescent dye in mice.

原文英語
頁(從 - 到)19-24
頁數6
期刊Pediatrics and Neonatology
56
發行號1
DOIs
出版狀態已發佈 - 二月 1 2015

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

指紋 深入研究「Effect of surfactant and budesonide on the pulmonary distribution of fluorescent dye in mice」主題。共同形成了獨特的指紋。

引用此