Effect of phytocompounds from the heartwood of Acacia confusa on inflammatory mediator production

Jyh Horng Wu, Yu Tang Tung, Shih Chang Chien, Sheng Yang Wang, Yueh Hsiung Kuo, Lie Fen Shyur, Shang Tzen Chang

研究成果: 雜誌貢獻文章同行評審

41 引文 斯高帕斯(Scopus)

摘要

Acacia confusa Merr. (Leguminosae) is traditionally used as a medicinal plant in Taiwan. In the present study, anti-inflammatory activity of extracts from the heartwood of A. confusa were investigated for the first time. Results demonstrated that ethanolic extracts of A. confusa heartwood strongly suppressed NO production in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages. Among all fractions derived from ethanolic extracts, the EtOAc fraction exhibited the best inhibitory activity. Following column chromatography and reverse-phase high-performance liquid chromatography, 13 specific phytocompounds including 5 new flavonoids (i.e., 7,8,3′,4′-tetrahydroxy-4- methoxyflavan-3-ol, 7,8,3′,4′-tetrahydroxyflavone, 7,8,3′-trihydroxy-3,4′-dimethoxyflavone, 7,3′,4′- trihydroxyflavone, and 7,3′,4′-trihydroxy-3-methoxyflavone) were isolated and identified from the EtOAc fraction. In addition, melanoxetin (3,7,8,3′,4′-pentahydroxyflavone), a major compound in the EtOAc fraction, markedly suppressed LPS-induced NO and prostaglandin E 2 (PGE 2) production. Moreover, melanoxetin completely suppressed gene expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) at 50 and 100 μM, respectively. This is the first report to identify the inhibitory bioactivities of melanoxetin on iNOS and COX-2.
原文英語
頁(從 - 到)1567-1573
頁數7
期刊Journal of Agricultural and Food Chemistry
56
發行號5
DOIs
出版狀態已發佈 - 三月 12 2008
對外發佈Yes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

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