Effect of incubation with different concentrations and durations of FSH for in-vitro maturation of murine oocytes

Yu Hung Lin, Jiann-Loung Hwang, Kok Min Seow, Lee Wen Huang, Bih Chwen Hsieh, Hen Ju Chen, Chii Ruey Tzeng, Chyi Huey Bai

研究成果: 雜誌貢獻文章

2 引文 (Scopus)

摘要

The purpose of the study was to evaluate whether a lower concentration of FSH or 2-h incubation with FSH would improve the outcome of in-vitro maturation of oocytes. The immature oocytes were obtained from FVB mice, and were allocated to four groups and incubated in the maturation media for 24 h. The maturation media were supplemented with 10 mIU/ml FSH for 24 h (group 1), 10 mIU/ml FSH for 2 h (group 2), 75 mIU/ml FSH for 24 h (group 3) or 75 mIU/ml FSH for 2 h (group 4). In each group, half of the in-vitro-matured oocytes were fertilized and cultured to blastocysts and the remaining matured oocytes were analysed for growth differentiation factor (GDF)-9 and bone morphogenetic protein (BMP)-15 mRNA to assess the oocyte quality. The maturation rates and oocyte BMP-15 mRNA concentrations were similar among the four groups. The GDF-9 mRNA concentrations were similar in group 2 and group 4. The fertilization and blastocyst rates were higher in groups 2 and 4 than in groups 1 and 3. It is concluded that 2-h incubation with FSH is better than 24-h incubation in terms of the fertilization rate and blastocyst development. Immature oocyte retrieval followed by in-vitro maturation (IVM) is a feasible alternative to IVF. However, the implantation rate of IVM remains lower than that of traditional IVF. The purpose of the study was to evaluate whether a lower concentration of FSH or 2-h incubation with FSH would improve the outcome of IVM. The Immature oocytes were obtained from mice, and were allocated to four groups and incubated in the maturation media for 24 h. The maturation media were supplemented with 10 mIU/ml FSH for 24 h (group 1), 10 mIU/ml FSH for 2 h (group 2), 75 mIU/ml FSH for 24 h (group 3) or 75 mIU/ml FSH for 2 h (group 4). In each group, half of the in-vitro-matured oocytes were fertilized and cultured to blastocysts and the remaining matured oocytes were analysed for growth differentiation factor (GDF)-9 and bone morphogenetic protein (BMP)-15 mRNA to assess the oocyte quality. The maturation rates and oocyte BMP-15 mRNA concentrations were similar between the four groups. The GDF-9 mRNA concentrations were similar in group 2 and group 4. The fertilization and blastocyst rates were higher in groups 2 and 4 than in groups 1 and 3. We conclude that 2-h incubation with FSH is better than 24-h incubation in terms of the fertilization rate and blastocyst development.
原文英語
頁(從 - 到)111-117
頁數7
期刊Reproductive BioMedicine Online
23
發行號1
DOIs
出版狀態已發佈 - 七月 2011

指紋

In Vitro Oocyte Maturation Techniques
Oocytes
Bone Morphogenetic Protein 15
Growth Differentiation Factor 9
Blastocyst
Fertilization
Messenger RNA
Oocyte Retrieval
In Vitro Techniques

ASJC Scopus subject areas

  • Reproductive Medicine
  • Developmental Biology

引用此文

Effect of incubation with different concentrations and durations of FSH for in-vitro maturation of murine oocytes. / Lin, Yu Hung; Hwang, Jiann-Loung; Seow, Kok Min; Huang, Lee Wen; Hsieh, Bih Chwen; Chen, Hen Ju; Tzeng, Chii Ruey; Bai, Chyi Huey.

於: Reproductive BioMedicine Online, 卷 23, 編號 1, 07.2011, p. 111-117.

研究成果: 雜誌貢獻文章

Lin, Yu Hung ; Hwang, Jiann-Loung ; Seow, Kok Min ; Huang, Lee Wen ; Hsieh, Bih Chwen ; Chen, Hen Ju ; Tzeng, Chii Ruey ; Bai, Chyi Huey. / Effect of incubation with different concentrations and durations of FSH for in-vitro maturation of murine oocytes. 於: Reproductive BioMedicine Online. 2011 ; 卷 23, 編號 1. 頁 111-117.
@article{48f7bac2821c471f98d83838213f8c7a,
title = "Effect of incubation with different concentrations and durations of FSH for in-vitro maturation of murine oocytes",
abstract = "The purpose of the study was to evaluate whether a lower concentration of FSH or 2-h incubation with FSH would improve the outcome of in-vitro maturation of oocytes. The immature oocytes were obtained from FVB mice, and were allocated to four groups and incubated in the maturation media for 24 h. The maturation media were supplemented with 10 mIU/ml FSH for 24 h (group 1), 10 mIU/ml FSH for 2 h (group 2), 75 mIU/ml FSH for 24 h (group 3) or 75 mIU/ml FSH for 2 h (group 4). In each group, half of the in-vitro-matured oocytes were fertilized and cultured to blastocysts and the remaining matured oocytes were analysed for growth differentiation factor (GDF)-9 and bone morphogenetic protein (BMP)-15 mRNA to assess the oocyte quality. The maturation rates and oocyte BMP-15 mRNA concentrations were similar among the four groups. The GDF-9 mRNA concentrations were similar in group 2 and group 4. The fertilization and blastocyst rates were higher in groups 2 and 4 than in groups 1 and 3. It is concluded that 2-h incubation with FSH is better than 24-h incubation in terms of the fertilization rate and blastocyst development. Immature oocyte retrieval followed by in-vitro maturation (IVM) is a feasible alternative to IVF. However, the implantation rate of IVM remains lower than that of traditional IVF. The purpose of the study was to evaluate whether a lower concentration of FSH or 2-h incubation with FSH would improve the outcome of IVM. The Immature oocytes were obtained from mice, and were allocated to four groups and incubated in the maturation media for 24 h. The maturation media were supplemented with 10 mIU/ml FSH for 24 h (group 1), 10 mIU/ml FSH for 2 h (group 2), 75 mIU/ml FSH for 24 h (group 3) or 75 mIU/ml FSH for 2 h (group 4). In each group, half of the in-vitro-matured oocytes were fertilized and cultured to blastocysts and the remaining matured oocytes were analysed for growth differentiation factor (GDF)-9 and bone morphogenetic protein (BMP)-15 mRNA to assess the oocyte quality. The maturation rates and oocyte BMP-15 mRNA concentrations were similar between the four groups. The GDF-9 mRNA concentrations were similar in group 2 and group 4. The fertilization and blastocyst rates were higher in groups 2 and 4 than in groups 1 and 3. We conclude that 2-h incubation with FSH is better than 24-h incubation in terms of the fertilization rate and blastocyst development.",
keywords = "BMP-15, c-AMP, FSH, GDF-9, in-vitro maturation",
author = "Lin, {Yu Hung} and Jiann-Loung Hwang and Seow, {Kok Min} and Huang, {Lee Wen} and Hsieh, {Bih Chwen} and Chen, {Hen Ju} and Tzeng, {Chii Ruey} and Bai, {Chyi Huey}",
year = "2011",
month = "7",
doi = "10.1016/j.rbmo.2011.03.019",
language = "English",
volume = "23",
pages = "111--117",
journal = "Reproductive BioMedicine Online",
issn = "1472-6483",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Effect of incubation with different concentrations and durations of FSH for in-vitro maturation of murine oocytes

AU - Lin, Yu Hung

AU - Hwang, Jiann-Loung

AU - Seow, Kok Min

AU - Huang, Lee Wen

AU - Hsieh, Bih Chwen

AU - Chen, Hen Ju

AU - Tzeng, Chii Ruey

AU - Bai, Chyi Huey

PY - 2011/7

Y1 - 2011/7

N2 - The purpose of the study was to evaluate whether a lower concentration of FSH or 2-h incubation with FSH would improve the outcome of in-vitro maturation of oocytes. The immature oocytes were obtained from FVB mice, and were allocated to four groups and incubated in the maturation media for 24 h. The maturation media were supplemented with 10 mIU/ml FSH for 24 h (group 1), 10 mIU/ml FSH for 2 h (group 2), 75 mIU/ml FSH for 24 h (group 3) or 75 mIU/ml FSH for 2 h (group 4). In each group, half of the in-vitro-matured oocytes were fertilized and cultured to blastocysts and the remaining matured oocytes were analysed for growth differentiation factor (GDF)-9 and bone morphogenetic protein (BMP)-15 mRNA to assess the oocyte quality. The maturation rates and oocyte BMP-15 mRNA concentrations were similar among the four groups. The GDF-9 mRNA concentrations were similar in group 2 and group 4. The fertilization and blastocyst rates were higher in groups 2 and 4 than in groups 1 and 3. It is concluded that 2-h incubation with FSH is better than 24-h incubation in terms of the fertilization rate and blastocyst development. Immature oocyte retrieval followed by in-vitro maturation (IVM) is a feasible alternative to IVF. However, the implantation rate of IVM remains lower than that of traditional IVF. The purpose of the study was to evaluate whether a lower concentration of FSH or 2-h incubation with FSH would improve the outcome of IVM. The Immature oocytes were obtained from mice, and were allocated to four groups and incubated in the maturation media for 24 h. The maturation media were supplemented with 10 mIU/ml FSH for 24 h (group 1), 10 mIU/ml FSH for 2 h (group 2), 75 mIU/ml FSH for 24 h (group 3) or 75 mIU/ml FSH for 2 h (group 4). In each group, half of the in-vitro-matured oocytes were fertilized and cultured to blastocysts and the remaining matured oocytes were analysed for growth differentiation factor (GDF)-9 and bone morphogenetic protein (BMP)-15 mRNA to assess the oocyte quality. The maturation rates and oocyte BMP-15 mRNA concentrations were similar between the four groups. The GDF-9 mRNA concentrations were similar in group 2 and group 4. The fertilization and blastocyst rates were higher in groups 2 and 4 than in groups 1 and 3. We conclude that 2-h incubation with FSH is better than 24-h incubation in terms of the fertilization rate and blastocyst development.

AB - The purpose of the study was to evaluate whether a lower concentration of FSH or 2-h incubation with FSH would improve the outcome of in-vitro maturation of oocytes. The immature oocytes were obtained from FVB mice, and were allocated to four groups and incubated in the maturation media for 24 h. The maturation media were supplemented with 10 mIU/ml FSH for 24 h (group 1), 10 mIU/ml FSH for 2 h (group 2), 75 mIU/ml FSH for 24 h (group 3) or 75 mIU/ml FSH for 2 h (group 4). In each group, half of the in-vitro-matured oocytes were fertilized and cultured to blastocysts and the remaining matured oocytes were analysed for growth differentiation factor (GDF)-9 and bone morphogenetic protein (BMP)-15 mRNA to assess the oocyte quality. The maturation rates and oocyte BMP-15 mRNA concentrations were similar among the four groups. The GDF-9 mRNA concentrations were similar in group 2 and group 4. The fertilization and blastocyst rates were higher in groups 2 and 4 than in groups 1 and 3. It is concluded that 2-h incubation with FSH is better than 24-h incubation in terms of the fertilization rate and blastocyst development. Immature oocyte retrieval followed by in-vitro maturation (IVM) is a feasible alternative to IVF. However, the implantation rate of IVM remains lower than that of traditional IVF. The purpose of the study was to evaluate whether a lower concentration of FSH or 2-h incubation with FSH would improve the outcome of IVM. The Immature oocytes were obtained from mice, and were allocated to four groups and incubated in the maturation media for 24 h. The maturation media were supplemented with 10 mIU/ml FSH for 24 h (group 1), 10 mIU/ml FSH for 2 h (group 2), 75 mIU/ml FSH for 24 h (group 3) or 75 mIU/ml FSH for 2 h (group 4). In each group, half of the in-vitro-matured oocytes were fertilized and cultured to blastocysts and the remaining matured oocytes were analysed for growth differentiation factor (GDF)-9 and bone morphogenetic protein (BMP)-15 mRNA to assess the oocyte quality. The maturation rates and oocyte BMP-15 mRNA concentrations were similar between the four groups. The GDF-9 mRNA concentrations were similar in group 2 and group 4. The fertilization and blastocyst rates were higher in groups 2 and 4 than in groups 1 and 3. We conclude that 2-h incubation with FSH is better than 24-h incubation in terms of the fertilization rate and blastocyst development.

KW - BMP-15

KW - c-AMP

KW - FSH

KW - GDF-9

KW - in-vitro maturation

UR - http://www.scopus.com/inward/record.url?scp=79960380763&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79960380763&partnerID=8YFLogxK

U2 - 10.1016/j.rbmo.2011.03.019

DO - 10.1016/j.rbmo.2011.03.019

M3 - Article

C2 - 21757130

AN - SCOPUS:79960380763

VL - 23

SP - 111

EP - 117

JO - Reproductive BioMedicine Online

JF - Reproductive BioMedicine Online

SN - 1472-6483

IS - 1

ER -