摘要
原文 | 英語 |
---|---|
頁(從 - 到) | 111-117 |
頁數 | 7 |
期刊 | Reproductive BioMedicine Online |
卷 | 23 |
發行號 | 1 |
DOIs | |
出版狀態 | 已發佈 - 七月 2011 |
指紋
ASJC Scopus subject areas
- Reproductive Medicine
- Developmental Biology
引用此文
Effect of incubation with different concentrations and durations of FSH for in-vitro maturation of murine oocytes. / Lin, Yu Hung; Hwang, Jiann-Loung; Seow, Kok Min; Huang, Lee Wen; Hsieh, Bih Chwen; Chen, Hen Ju; Tzeng, Chii Ruey; Bai, Chyi Huey.
於: Reproductive BioMedicine Online, 卷 23, 編號 1, 07.2011, p. 111-117.研究成果: 雜誌貢獻 › 文章
}
TY - JOUR
T1 - Effect of incubation with different concentrations and durations of FSH for in-vitro maturation of murine oocytes
AU - Lin, Yu Hung
AU - Hwang, Jiann-Loung
AU - Seow, Kok Min
AU - Huang, Lee Wen
AU - Hsieh, Bih Chwen
AU - Chen, Hen Ju
AU - Tzeng, Chii Ruey
AU - Bai, Chyi Huey
PY - 2011/7
Y1 - 2011/7
N2 - The purpose of the study was to evaluate whether a lower concentration of FSH or 2-h incubation with FSH would improve the outcome of in-vitro maturation of oocytes. The immature oocytes were obtained from FVB mice, and were allocated to four groups and incubated in the maturation media for 24 h. The maturation media were supplemented with 10 mIU/ml FSH for 24 h (group 1), 10 mIU/ml FSH for 2 h (group 2), 75 mIU/ml FSH for 24 h (group 3) or 75 mIU/ml FSH for 2 h (group 4). In each group, half of the in-vitro-matured oocytes were fertilized and cultured to blastocysts and the remaining matured oocytes were analysed for growth differentiation factor (GDF)-9 and bone morphogenetic protein (BMP)-15 mRNA to assess the oocyte quality. The maturation rates and oocyte BMP-15 mRNA concentrations were similar among the four groups. The GDF-9 mRNA concentrations were similar in group 2 and group 4. The fertilization and blastocyst rates were higher in groups 2 and 4 than in groups 1 and 3. It is concluded that 2-h incubation with FSH is better than 24-h incubation in terms of the fertilization rate and blastocyst development. Immature oocyte retrieval followed by in-vitro maturation (IVM) is a feasible alternative to IVF. However, the implantation rate of IVM remains lower than that of traditional IVF. The purpose of the study was to evaluate whether a lower concentration of FSH or 2-h incubation with FSH would improve the outcome of IVM. The Immature oocytes were obtained from mice, and were allocated to four groups and incubated in the maturation media for 24 h. The maturation media were supplemented with 10 mIU/ml FSH for 24 h (group 1), 10 mIU/ml FSH for 2 h (group 2), 75 mIU/ml FSH for 24 h (group 3) or 75 mIU/ml FSH for 2 h (group 4). In each group, half of the in-vitro-matured oocytes were fertilized and cultured to blastocysts and the remaining matured oocytes were analysed for growth differentiation factor (GDF)-9 and bone morphogenetic protein (BMP)-15 mRNA to assess the oocyte quality. The maturation rates and oocyte BMP-15 mRNA concentrations were similar between the four groups. The GDF-9 mRNA concentrations were similar in group 2 and group 4. The fertilization and blastocyst rates were higher in groups 2 and 4 than in groups 1 and 3. We conclude that 2-h incubation with FSH is better than 24-h incubation in terms of the fertilization rate and blastocyst development.
AB - The purpose of the study was to evaluate whether a lower concentration of FSH or 2-h incubation with FSH would improve the outcome of in-vitro maturation of oocytes. The immature oocytes were obtained from FVB mice, and were allocated to four groups and incubated in the maturation media for 24 h. The maturation media were supplemented with 10 mIU/ml FSH for 24 h (group 1), 10 mIU/ml FSH for 2 h (group 2), 75 mIU/ml FSH for 24 h (group 3) or 75 mIU/ml FSH for 2 h (group 4). In each group, half of the in-vitro-matured oocytes were fertilized and cultured to blastocysts and the remaining matured oocytes were analysed for growth differentiation factor (GDF)-9 and bone morphogenetic protein (BMP)-15 mRNA to assess the oocyte quality. The maturation rates and oocyte BMP-15 mRNA concentrations were similar among the four groups. The GDF-9 mRNA concentrations were similar in group 2 and group 4. The fertilization and blastocyst rates were higher in groups 2 and 4 than in groups 1 and 3. It is concluded that 2-h incubation with FSH is better than 24-h incubation in terms of the fertilization rate and blastocyst development. Immature oocyte retrieval followed by in-vitro maturation (IVM) is a feasible alternative to IVF. However, the implantation rate of IVM remains lower than that of traditional IVF. The purpose of the study was to evaluate whether a lower concentration of FSH or 2-h incubation with FSH would improve the outcome of IVM. The Immature oocytes were obtained from mice, and were allocated to four groups and incubated in the maturation media for 24 h. The maturation media were supplemented with 10 mIU/ml FSH for 24 h (group 1), 10 mIU/ml FSH for 2 h (group 2), 75 mIU/ml FSH for 24 h (group 3) or 75 mIU/ml FSH for 2 h (group 4). In each group, half of the in-vitro-matured oocytes were fertilized and cultured to blastocysts and the remaining matured oocytes were analysed for growth differentiation factor (GDF)-9 and bone morphogenetic protein (BMP)-15 mRNA to assess the oocyte quality. The maturation rates and oocyte BMP-15 mRNA concentrations were similar between the four groups. The GDF-9 mRNA concentrations were similar in group 2 and group 4. The fertilization and blastocyst rates were higher in groups 2 and 4 than in groups 1 and 3. We conclude that 2-h incubation with FSH is better than 24-h incubation in terms of the fertilization rate and blastocyst development.
KW - BMP-15
KW - c-AMP
KW - FSH
KW - GDF-9
KW - in-vitro maturation
UR - http://www.scopus.com/inward/record.url?scp=79960380763&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79960380763&partnerID=8YFLogxK
U2 - 10.1016/j.rbmo.2011.03.019
DO - 10.1016/j.rbmo.2011.03.019
M3 - Article
C2 - 21757130
AN - SCOPUS:79960380763
VL - 23
SP - 111
EP - 117
JO - Reproductive BioMedicine Online
JF - Reproductive BioMedicine Online
SN - 1472-6483
IS - 1
ER -