Effect of hypervolaemic haemodilution on cerebral glutamate, glycerol, lactate and free radicals in heatstroke rats

Chen Kuei Chang, Wen Ta Chiu, Ching Ping Chang, Mao Tsun Lin

研究成果: 雜誌貢獻文章

22 引文 (Scopus)

摘要

In the present study, we attempted to assess the mechanisms underlying the neuroprotective effect of hypervolaemic haemodilution in rat heatstroke. In anaesthetized rats treated with normal saline (NS) immediately after the onset of heatstroke induced by Ta (ambient temperature) of 42°C for 88 min, followed by Ta of 24°C for 12 min, the values for MAP (mean arterial pressure), ICP (intracranial pressure), CPP (cerebral perfusion pressure), CBF (cerebral blood blow), brain PO2 (partial pressure of O2) and striatal glutamate, glycerol, lactate/pyruvate ratio, hydroxyl radicals and neuronal damage score were 42 ± 3 mmHg, 33 ± 3 mmHg, 9 ± 3 mmHg, 109 ± 20 BPU (blood perfusion units), 6 ± 1 mmHg, 51 ± 7 μmol/l, 24 ± 3 μmol/l, 124 ± 32, 694 ± 22% of baseline and 2.25 ± 0.05 respectively. In animals treated with 10% albumin immediately after the onset of heatstroke (T a of 42°C for 88 min), the values for MAP, ICP, CPP, CBF, brain PO2 and striatal glutamate, glycerol, lactate/pyruvate ratio, hydroxyl radicals and neuronal damage score were 64 ± 6 mmHg, 10 ± 2 mmHg, 54 ± 5 mmHg, 452 ± 75 BPU, 15 ± 2 mmHg, 3 ± 2 μmol/l, 4 ± 2 μmol/l, 7 ± 3, 119 ± 7% of baseline and 0.38 ± 0.05 respectively. Apparently, the heatstroke-induced arterial hypotension, intracranial hypertension, cerebral hypoperfusion, cerebral ischaemia, brain hypoxia, increased levels of striatal glutamate, glycerol, lactate/pyruvate ratio and hydroxyl radicals, and increased striatal neuronal damage score values were all attenuated significantly by the induction of hypervolaemic haemodilution in rats immediately at the onset of heatstroke. These results demonstrate that the neuroprotective effect of hypervolaemic haemodilution is associated with a decrease in the elevation of glutamate, glycerol, lactate and free radicals in brain exposed to experimental heatstroke-induced cerebral ischaemia/hypoxia injury.
原文英語
頁(從 - 到)501-509
頁數9
期刊Clinical Science
106
發行號5
DOIs
出版狀態已發佈 - 五月 2004

指紋

Heat Stroke
Hemodilution
Glycerol
Free Radicals
Corpus Striatum
Glutamic Acid
Lactic Acid
Cerebrovascular Circulation
Pyruvic Acid
Hydroxyl Radical
Brain Hypoxia-Ischemia
Partial Pressure
Intracranial Pressure
Neuroprotective Agents
Arterial Pressure
Brain
Perfusion
Controlled Hypotension
Brain Hypoxia
Temperature

ASJC Scopus subject areas

  • Medicine(all)

引用此文

Effect of hypervolaemic haemodilution on cerebral glutamate, glycerol, lactate and free radicals in heatstroke rats. / Chang, Chen Kuei; Chiu, Wen Ta; Chang, Ching Ping; Lin, Mao Tsun.

於: Clinical Science, 卷 106, 編號 5, 05.2004, p. 501-509.

研究成果: 雜誌貢獻文章

Chang, Chen Kuei ; Chiu, Wen Ta ; Chang, Ching Ping ; Lin, Mao Tsun. / Effect of hypervolaemic haemodilution on cerebral glutamate, glycerol, lactate and free radicals in heatstroke rats. 於: Clinical Science. 2004 ; 卷 106, 編號 5. 頁 501-509.
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abstract = "In the present study, we attempted to assess the mechanisms underlying the neuroprotective effect of hypervolaemic haemodilution in rat heatstroke. In anaesthetized rats treated with normal saline (NS) immediately after the onset of heatstroke induced by Ta (ambient temperature) of 42°C for 88 min, followed by Ta of 24°C for 12 min, the values for MAP (mean arterial pressure), ICP (intracranial pressure), CPP (cerebral perfusion pressure), CBF (cerebral blood blow), brain PO2 (partial pressure of O2) and striatal glutamate, glycerol, lactate/pyruvate ratio, hydroxyl radicals and neuronal damage score were 42 ± 3 mmHg, 33 ± 3 mmHg, 9 ± 3 mmHg, 109 ± 20 BPU (blood perfusion units), 6 ± 1 mmHg, 51 ± 7 μmol/l, 24 ± 3 μmol/l, 124 ± 32, 694 ± 22{\%} of baseline and 2.25 ± 0.05 respectively. In animals treated with 10{\%} albumin immediately after the onset of heatstroke (T a of 42°C for 88 min), the values for MAP, ICP, CPP, CBF, brain PO2 and striatal glutamate, glycerol, lactate/pyruvate ratio, hydroxyl radicals and neuronal damage score were 64 ± 6 mmHg, 10 ± 2 mmHg, 54 ± 5 mmHg, 452 ± 75 BPU, 15 ± 2 mmHg, 3 ± 2 μmol/l, 4 ± 2 μmol/l, 7 ± 3, 119 ± 7{\%} of baseline and 0.38 ± 0.05 respectively. Apparently, the heatstroke-induced arterial hypotension, intracranial hypertension, cerebral hypoperfusion, cerebral ischaemia, brain hypoxia, increased levels of striatal glutamate, glycerol, lactate/pyruvate ratio and hydroxyl radicals, and increased striatal neuronal damage score values were all attenuated significantly by the induction of hypervolaemic haemodilution in rats immediately at the onset of heatstroke. These results demonstrate that the neuroprotective effect of hypervolaemic haemodilution is associated with a decrease in the elevation of glutamate, glycerol, lactate and free radicals in brain exposed to experimental heatstroke-induced cerebral ischaemia/hypoxia injury.",
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N2 - In the present study, we attempted to assess the mechanisms underlying the neuroprotective effect of hypervolaemic haemodilution in rat heatstroke. In anaesthetized rats treated with normal saline (NS) immediately after the onset of heatstroke induced by Ta (ambient temperature) of 42°C for 88 min, followed by Ta of 24°C for 12 min, the values for MAP (mean arterial pressure), ICP (intracranial pressure), CPP (cerebral perfusion pressure), CBF (cerebral blood blow), brain PO2 (partial pressure of O2) and striatal glutamate, glycerol, lactate/pyruvate ratio, hydroxyl radicals and neuronal damage score were 42 ± 3 mmHg, 33 ± 3 mmHg, 9 ± 3 mmHg, 109 ± 20 BPU (blood perfusion units), 6 ± 1 mmHg, 51 ± 7 μmol/l, 24 ± 3 μmol/l, 124 ± 32, 694 ± 22% of baseline and 2.25 ± 0.05 respectively. In animals treated with 10% albumin immediately after the onset of heatstroke (T a of 42°C for 88 min), the values for MAP, ICP, CPP, CBF, brain PO2 and striatal glutamate, glycerol, lactate/pyruvate ratio, hydroxyl radicals and neuronal damage score were 64 ± 6 mmHg, 10 ± 2 mmHg, 54 ± 5 mmHg, 452 ± 75 BPU, 15 ± 2 mmHg, 3 ± 2 μmol/l, 4 ± 2 μmol/l, 7 ± 3, 119 ± 7% of baseline and 0.38 ± 0.05 respectively. Apparently, the heatstroke-induced arterial hypotension, intracranial hypertension, cerebral hypoperfusion, cerebral ischaemia, brain hypoxia, increased levels of striatal glutamate, glycerol, lactate/pyruvate ratio and hydroxyl radicals, and increased striatal neuronal damage score values were all attenuated significantly by the induction of hypervolaemic haemodilution in rats immediately at the onset of heatstroke. These results demonstrate that the neuroprotective effect of hypervolaemic haemodilution is associated with a decrease in the elevation of glutamate, glycerol, lactate and free radicals in brain exposed to experimental heatstroke-induced cerebral ischaemia/hypoxia injury.

AB - In the present study, we attempted to assess the mechanisms underlying the neuroprotective effect of hypervolaemic haemodilution in rat heatstroke. In anaesthetized rats treated with normal saline (NS) immediately after the onset of heatstroke induced by Ta (ambient temperature) of 42°C for 88 min, followed by Ta of 24°C for 12 min, the values for MAP (mean arterial pressure), ICP (intracranial pressure), CPP (cerebral perfusion pressure), CBF (cerebral blood blow), brain PO2 (partial pressure of O2) and striatal glutamate, glycerol, lactate/pyruvate ratio, hydroxyl radicals and neuronal damage score were 42 ± 3 mmHg, 33 ± 3 mmHg, 9 ± 3 mmHg, 109 ± 20 BPU (blood perfusion units), 6 ± 1 mmHg, 51 ± 7 μmol/l, 24 ± 3 μmol/l, 124 ± 32, 694 ± 22% of baseline and 2.25 ± 0.05 respectively. In animals treated with 10% albumin immediately after the onset of heatstroke (T a of 42°C for 88 min), the values for MAP, ICP, CPP, CBF, brain PO2 and striatal glutamate, glycerol, lactate/pyruvate ratio, hydroxyl radicals and neuronal damage score were 64 ± 6 mmHg, 10 ± 2 mmHg, 54 ± 5 mmHg, 452 ± 75 BPU, 15 ± 2 mmHg, 3 ± 2 μmol/l, 4 ± 2 μmol/l, 7 ± 3, 119 ± 7% of baseline and 0.38 ± 0.05 respectively. Apparently, the heatstroke-induced arterial hypotension, intracranial hypertension, cerebral hypoperfusion, cerebral ischaemia, brain hypoxia, increased levels of striatal glutamate, glycerol, lactate/pyruvate ratio and hydroxyl radicals, and increased striatal neuronal damage score values were all attenuated significantly by the induction of hypervolaemic haemodilution in rats immediately at the onset of heatstroke. These results demonstrate that the neuroprotective effect of hypervolaemic haemodilution is associated with a decrease in the elevation of glutamate, glycerol, lactate and free radicals in brain exposed to experimental heatstroke-induced cerebral ischaemia/hypoxia injury.

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